A cyclic‐di‐GMP receptor required for bacterial exopolysaccharide production

Lee, Vincent T.; Matewish, Jody M.; Kessler, Jennifer; Hyodo, Mamoru; Hayakawa, Yoshihiro; Lory, Stephen

doi:10.1111/j.1365-2958.2007.05879.x

Cited by 395 publications

(442 citation statements)

References 57 publications

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“…While activating flagellum gene expression, FleQ represses pel transcription at low c-di-GMP levels (under planktonic, free-swimming growth conditions), with its inhibitory effect on pel transcription negated when intracellular c-di-GMP levels are high (60). Pel polysaccharide biosynthesis is furthermore regulated in P. aeruginosa by the c-di-GMP receptor protein PelD (91). Recently, a novel c-di-GMP protein that triggers attachment by inducing polysaccharide production was identified in V. cholerae (83).…”

Section: Modulation Of Cyclic Di-gmpmentioning

confidence: 99%

Sticky Situations: Key Components That Control Bacterial Surface Attachment

Petrova

Sauer

2012

Journal of Bacteriology

319

251

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Section: Modulation Of Cyclic Di-gmpmentioning

confidence: 99%

Sticky Situations: Key Components That Control Bacterial Surface Attachment

Petrova

Sauer

2012

Journal of Bacteriology

319

251

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“…A modified Congo red binding assay, described previously by Lee et al (2007), was used to assess the effect of CFS on pathogenic exopolysaccharide. The bacterial cells were collected after centrifugation, and resuspended in 1 mL LB broth containing Congo red (40 μg/mL) and 100 μL CFS derived from lactobacilli.…”

Section: Effect Of Cfs Derived From Lactobacilli On Pathogenic Extracmentioning

confidence: 99%

Lactic acid bacteria protect human intestinal epithelial cells from Staphylococcus aureus and Pseudomonas aeruginosa infections

Affhan¹,

Dachang²,

Xin³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Staphylococcus aureus and Pseudomonas aeruginosa are opportunistic pathogens that cause nosocomial and food-borne infections. They promote intestinal diseases. Gastrointestinal colonization by S. aureus and P. aeruginosa has rarely been researched. These organisms spread to extra gastrointestinal niches, resulting in increasingly progressive infections. Lactic acid bacteria are Gram-positive bacteria that produce lactic acid as the major end-product of carbohydrate fermentation. These bacteria inhibit pathogen colonization and modulate the host immune response. This study aimed to investigate the effects of Lactobacillus acidophilus and Lactobacillus rhamnosus on enteric infections caused by the paradigmatic human pathogens S. aureus ATCC25923 and P. aeruginosa ATCC27853. The effect of whole cells and neutralized cell-free supernatant (CFS) of the lactobacilli on LoVo human carcinoma enterocyte (ATCC CCL-229) infection was analyzed by co-exposure, pre-exposure, and post-exposure studies. Simultaneous application of whole cells and CFS of the lactobacilli significantly eradicated enterocyte infection (P < 0.05); however, this effect was not seen when the whole cells and CFS 17044-17058 (2015) were added after or prior to the infection (P > 0.05). This result could be attributed to interference by extracellular polymeric substances and cell surface hydrophobicity, which resulted in the development of a pathogen that did not form colonies. Furthermore, results of the plate count and LIVE/ DEAD BacLight bacterial viability staining attributed this inhibition to a nonbacteriocin-like substance, which acted independently of organic acid and H 2 O 2 production. Based on these results, the cell-free supernatant derived from lactobacilli was concluded to restrain the development of S. aureus and P. aeruginosa enteric infections.

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“…Another well-documented activity of cyclic di-GMP in P. aeruginosa is the induction of biofilm formation (Hickman et al, 2005;Lee et al, 2007). Elevated cellular levels of cyclic di-GMP increase the production of biofilm matrix components in P. aeruginosa including the Pel and Psl polysaccharides and a biofilm-associated adhesin (Hickman et al, 2005;Hengge, 2009;Borlee et al, 2010).…”

Section: Ml1419c Tpbb Ml1419cmentioning

confidence: 99%

“…To overcome these limitations, we chose to express ml1419c in an alternative heterologous host, P. aeruginosa PAO1, in which the effects of increasing and decreasing levels of cyclic di-GMP are well documented and control a range of phenotypes (Borlee et al, 2010;Hickman & Harwood, 2008;Hickman et al, 2005;Irie et al, 2012;Kulasakara et al, 2006;Merritt et al, 2007;Lee et al, 2007;Mills et al, 2011). Previously published studies have specifically shown that overexpression of the DGC encoded by tpbB (PA1120) increases cyclic di-GMP concentrations and corresponding biofilm formation in P. aeruginosa (Hickman & Harwood, 2008;Kulasakara et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Diguanylate cyclase activity of the Mycobacterium leprae T cell antigen ML1419c

et al. 2016

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The second messenger, bis-(3¢,5¢)-cyclic dimeric guanosine monophosphate (cyclic di-GMP), is involved in the control of multiple bacterial phenotypes, including those that impact host-pathogen interactions. Bioinformatics analyses predicted that Mycobacterium leprae, an obligate intracellular bacterium and the causative agent of leprosy, encodes three active diguanylate cyclases. In contrast, the related pathogen Mycobacterium tuberculosis encodes only a single diguanylate cyclase. One of the M. leprae unique diguanylate cyclases (ML1419c) was previously shown to be produced early during the course of leprosy. Thus, functional analysis of ML1419c was performed. The gene encoding ML1419c was cloned and expressed in Pseudomonas aeruginosa PAO1 to allow for assessment of cyclic di-GMP production and cyclic di-GMP-mediated phenotypes. Phenotypic studies revealed that ml1419c expression altered colony morphology, motility and biofilm formation of P. aeruginosa PAO1 in a manner consistent with increased cyclic di-GMP production. Direct measurement of cyclic di-GMP levels by liquid chromatography-mass spectrometry confirmed that ml1419c expression increased cyclic di-GMP production in P. aeruginosa PAO1 cultures in comparison to the vector control. The observed phenotypes and increased levels of cyclic di-GMP detected in P. aeruginosa expressing ml1419c could be abrogated by mutation of the active site in ML1419c. These studies demonstrated that ML1419c of M. leprae functions as diguanylate cyclase to synthesize cyclic di-GMP. Thus, this protein was renamed DgcA (Diguanylate cyclase A). These results also demonstrated the ability to use P. aeruginosa as a heterologous host for characterizing the function of proteins involved in the cyclic di-GMP pathway of a pathogen refractory to in vitro growth, M. leprae.

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A cyclic‐di‐GMP receptor required for bacterial exopolysaccharide production

Cited by 395 publications

References 57 publications

Sticky Situations: Key Components That Control Bacterial Surface Attachment

Sticky Situations: Key Components That Control Bacterial Surface Attachment

Lactic acid bacteria protect human intestinal epithelial cells from Staphylococcus aureus and Pseudomonas aeruginosa infections

Diguanylate cyclase activity of the Mycobacterium leprae T cell antigen ML1419c

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