A Critical Assessment of the Congruency between Environmental DNA and Palaeoecology for the Biodiversity Monitoring and Palaeoenvironmental Reconstruction

Abstract

The present study suggests that standardized methodology, careful site selection, and stratigraphy are essential for investigating ancient ecosystems in order to evaluate biodiversity and DNA-based time series. Based on specific keywords, this investigation reviewed 146 publications using the SCOPUS, Web of Science (WoS), PUBMED, and Google Scholar databases. Results indicate that environmental deoxyribose nucleic acid (eDNA) can be pivotal for assessing and conserving ecosystems. Our review revealed that in the last 12 years (January 2008–July 2021), 63% of the studies based on eDNA have been reported from aquatic ecosystems, 25% from marine habitats, and 12% from terrestrial environments. Out of studies conducted in aquatic systems using the environmental DNA (eDNA) technique, 63% of the investigations have been reported from freshwater ecosystems, with an utmost focus on fish diversity (40%). Further analysis of the literature reveals that during the same period, 24% of the investigations using the environmental DNA technique were carried out on invertebrates, 8% on mammals, 7% on plants, 6% on reptiles, and 5% on birds. The results obtained clearly indicate that the environmental DNA technique has a clear-cut edge over other biodiversity monitoring methods. Furthermore, we also found that eDNA, in conjunction with different dating techniques, can provide better insight into deciphering eco-evolutionary feedback. Therefore, an attempt has been made to offer extensive information on the application of dating methods for different taxa present in diverse ecosystems. Last, we provide suggestions and elucidations on how to overcome the caveats and delineate some of the research avenues that will likely shape this field in the near future. This paper aims to identify the gaps in environmental DNA (eDNA) investigations to help researchers, ecologists, and decision-makers to develop a holistic understanding of environmental DNA (eDNA) and its utility as a palaeoenvironmental contrivance.

Estimation of Nuclear DNA Content in Some Aegilops Species: Best Analyzed Using Flow Cytometry

“…Despite the large variation at the interspecific level, great stability could be observed at the intraspecific level and all species with the same genomic constitution had similar DNA content. The results thus support conclusions of others researchers [ 42 , 50 , 51 , 52 , 53 ]. Our results were also consistent with Lee et al [ 54 ], which used flow cytometry and the chromosome imaging technique in hexaploid wheat, hexaploid triticale, tetraploid wheat and AA, BB, DD genome donors.…”

Environmental DNA Metabarcoding: A Novel Contrivance for Documenting Terrestrial Biodiversity

“…However, to achieve the desirable outcomes, it is necessary to design repeatable and reliable biodiversity surveys based on eDNA. It is important to understand the constraints and caveats that affect its implementation [ 29 ]. After thoroughly understanding its strengths and limitations, it can be possible to generate correct inferences from the data.…”

Client influence in property valuation: a scoping literature review

“…The use of Web of Science, Scopus and other approaches to searching for articles is to balance the unique strengths and weakness of each database, as some databases do not cover some fields or journals (see Adriaanse and Rensleigh, 2013; Murgado-Armenteros et al. , 2015; Hassan et al. , 2022).…”

“…Other relevant articles were included through citation searching within the obtained articles and search on Google Scholar. The use of Web of Science, Scopus and other approaches to searching for articles is to balance the unique strengths and weakness of each database, as some databases do not cover some fields or journals (see Adriaanse and Rensleigh, 2013;Murgado-Armenteros et al, 2015;Hassan et al, 2022). Considering the literature found within the domain and the choice of the scoping PM 41,5 review, every piece of literature focusing on client influence or pressure on property valuation was considered.…”