A continuous-flow high-throughput microfluidic device for airborne bacteria PCR detection

Jiang, Xiran; Jing, Wenwen; Zheng, Lulu; Liu, Sixiu; Wu, Wenjuan; Sui, Guodong

doi:10.1039/c3lc50977j

Cited by 55 publications

(34 citation statements)

References 22 publications

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“…The state of each valve can be precisely controlled (Fig. 1C), and the entire operation was programmed with LabView in semi-automatic and fully automatic modes [21]. The system could be used by technicians with minimum training, because minimal human intervention is required for the operation.…”

Section: General Setupmentioning

confidence: 99%

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Microfluidic system for high-throughput immunoglobulin-E analysis from clinical serum samples

Zheng

Jiang

et al. 2015

Talanta

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Section: General Setupmentioning

confidence: 99%

“…Various immunoassays [18] that have been miniaturized into microfluidic chips have the advantages of minimal reagent consumption, high-throughput operation, low cost, easy automation, and portability [19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Microfluidic system for high-throughput immunoglobulin-E analysis from clinical serum samples

Zheng

Jiang

et al. 2015

Talanta

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“…Jiang et al created a nearly fully automated continuous flowthrough microfluidic apparatus that was capable of bioaerosol capture and detection of six separate aerosolized bacterial targets. 162 Including sampling, lysis, and on-chip nucleic acid amplification, the total assay time was 1 h 10 min, with a detection limit <10 3 CFUs per mL. Separate gel electrophoresis was required for detection, but existing methods can be implemented into this protocol for (relatively) rapid detection.…”

Section: Pcr and Nucleic Acid Detectionmentioning

confidence: 99%

Biosensors for Monitoring Airborne Pathogens

Fronczek

Yoon

2015

SLAS Technology

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Airborne pathogens affect both humans and animals and are often highly and rapidly transmittable. Many problematic airborne pathogens, both viral (influenza A/H1N1, Rubella, and avian influenza/H5N1) and bacterial (Mycobacterium tuberculosis, Streptococcus pneumoniae, and Bacillus anthracis), have huge impacts on health care and agricultural applications, and can potentially be used as bioterrorism agents. Many different laboratory-based methods have been introduced and are currently being used. However, such detection is generally limited by sample collection, including nasal swabs and blood analysis. Direct identification from air (specifically, aerosol samples) would be ideal, but such detection has not been very successful due to the difficulty in sample collection and the extremely low pathogen concentration found in aerosol samples. In this review, we will discuss the portable biosensors and/or micro total analysis systems (µTAS) that can be used for monitoring such airborne pathogens, similar to smoke detectors. Current laboratory-based methods will be reviewed, and possible solutions to convert these lab-based methods into µTAS biosensors will be discussed.

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“…In designing a system, concentration rate should be optimized by increasing the air flow rate and collection efficiency, or decreasing the volume of capture liquid preferably to microfluidic volumes, because this improves the aerosol detection sensitivity. Past studies reconciling microfluidics with aerosol collection (by deposition of aerosol in channels/half-channels) have reported concentration rates of 10 4 -10 6 min ¡1 , and have delineated the limitations with these approaches (Han and Mainelis 2008;Han et al 2010Han et al , 2011Jing et al 2013Jing et al , 2014Pardon et al 2015;Han et al 2015a,b;Foat et al 2016;Ma et al 2016). These studies have shown successes, but require further work in integration with assay platforms, clearly demonstrating end-to-end bioaerosol collection and identification, estimating limits of detection, and detailed field testing.…”

Section: Introductionmentioning

confidence: 99%

Droplet-based microfluidics detector for bioaerosol detection

Damit

2017

Aerosol Science and Technology

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Detection of bioaerosols is important in fields ranging from environmental health monitoring to biosurveillance, and current detector weaknesses have motivated the development of new technologies. In this work, a detector was built, which applies the principles of droplet microfluidics to bioaerosol detection. Droplet microfluidics is a subfield of microfluidics based on the creation of monodisperse microdroplets with compartmentalized reagents and supports enhanced assays and fluidic manipulations. The bioaerosol detector operates by aerodynamically focusing aerosols directly into these droplets to harness the benefits of the microreactor environment. A breadboard detector system, which consisted of an aerodynamic focusing lens, aerosol-focusing capillary, microfluidic droplet chip, and optical microscope, was constructed. Computational fluid dynamic simulations and Lagrangian particle tracking modeling were conducted to identify the optimal conditions for focusing. Preliminary experiments, where aerosols were deposited onto a solid substrate, demonstrated sub 200-mm spot diameters for aerodynamic diameters of 2-5 mm. Test aerosols were then generated, and collected into the microfluidic liquid interface on the chip as verified by microscopy. Recovery efficiency of the aerosols was dependent on aerosol size and ranged from about 27% to nearly 100%. Finally, to prove bioaerosol collection and detection, a droplet propidium iodide (PI) assay was performed: the system distinguished between E. coli and non-biological aerosols within 20 s. Overall, this work established the technique of direct collection of bioaerosols into a convenient droplet microfluidic platform for detection. EDITORTiina Reponen

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A continuous-flow high-throughput microfluidic device for airborne bacteria PCR detection

Cited by 55 publications

References 22 publications

Microfluidic system for high-throughput immunoglobulin-E analysis from clinical serum samples

Microfluidic system for high-throughput immunoglobulin-E analysis from clinical serum samples

Biosensors for Monitoring Airborne Pathogens

Droplet-based microfluidics detector for bioaerosol detection

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