A continuous cell line from the cultured marine fish gilt-head seabream (Sparus aurata L.)

Béjar, Julia; Borrego, Juan J.; Álvarez, M. Carmen

doi:10.1016/s0044-8486(96)01469-x

Cited by 117 publications

(84 citation statements)

References 13 publications

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“…The established cell line SAF1 (Bejar et al, 1997) was routinely propagated and maintained at 25˚C in Leibovitz's L-15 with GlutaMAX™-l supplemented with kanamycin (100 µg/ml), penicillin G (50 U/ml), streptomycin (50 µg/ml) and 10% of fetal bovine serum (FBS). All components were purchased from Gibco®/Invitrogen, UK.…”

Section: Saf1 Cell Culturementioning

confidence: 99%

“…In the present study we report the transcriptional responses to sub-lethal Cu exposure in a fibroblast cell line from Sparus aurata fibroblast (SAF1) (Bejar et al, 1997), in order to assess its suitability as an in vitro system for studying Cu homeostasis and to gain insight into the mechanisms specifically involved in Cu-response in sea bream. We compared the responses of specific Cu-homeostasis and oxidative stress genes after exposure to different metals by quantitative RT-PCR and also carried out an analysis of broader effects by use of a medium density cDNA microarray from a closely related fish species, the striped sea bream (Lithognathus marmoratus).…”

Section: Introductionmentioning

confidence: 99%

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Copper induces Cu-ATPase ATP7A mRNA in a fish cell line, SAF1

Minghetti

Leaver

Taggart

et al. 2011

Comparative Biochemistry and Physiology Part C: Toxicology &

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Section: Saf1 Cell Culturementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Copper induces Cu-ATPase ATP7A mRNA in a fish cell line, SAF1

Minghetti

Leaver

Taggart

et al. 2011

Comparative Biochemistry and Physiology Part C: Toxicology &

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“…Initially, fibroblastic and epithelial cells were found in both the cell lines, but after several subcultures, fibroblastlike cells predominated in both the cell lines. Usually, a predomination of fibroblastic cells over epithelioid cells in cell cultures from fish has been reported (Bejar et al 1997;Lai et al 2003;Lakra et al 2006a). Ye et al (2006) developed a fibroblast-like cell line (LJH-2) from Lateolabrax japonicus.…”

Section: Resultsmentioning

confidence: 99%

“…The cell doubling time reported in the continuous cell line SAF-1 from Sparus aurata, RTG-2 cell line, PSM-1cell line from a hybrid between Xiphophorus maculatus and Xiphophorus hellen was 48 h (Plumb and Wolf 1971;Wakamatsu 1981;Bejar et . 1997).…”

Section: Resultsmentioning

confidence: 99%

Development and characterization of two cell lines PDF and PDH from Puntius denisonii (Day 1865)

Lakra

Goswami

Yadav

et al. 2010

In Vitro Cell.Dev.Biol.-Animal

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The Puntius denisonii colloquially and more popularly referred to as Miss Kerala is a subtropical fish belonging to the genus Puntius (Barb) and family Cyprinidae. Two cell lines PDF and PDH were developed from the caudal fin and heart of P. denisonii, respectively. The cell lines were optimally maintained at 26°C in Leibovitz-15 medium supplemented with 10% fetal bovine serum. A diploid count of 50 chromosomes at passage 50 was observed in both the cell lines. The high growth potential of the cell lines was reflected from the cell doubling time of 28 and 30 h of PDF and PDH cell lines, respectively. The viability of the PDF and PDH cell lines was 70% and 76%, respectively, after 4 mo of storage in liquid nitrogen (−196°C). The origin of the cell lines was confirmed by the amplification of 653 bp fragments of cytochrome oxidase subunit I of mitochondrial DNA genes.

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“…For these cells, a direct estimation on the length of the cell-cycle is not reported in the literature. Direct and indirect estimations on cell-cycle dynamics of different cell types, both from early studies on in vivo proliferating systems [44] and from explanted cell cultures [45,46] indicate an average cell-cycle length of about 3 days. Based on radioisotope labelling experiments, an average lifespan of about 150 days has been estimated for red blood cells in Tinca tinca [47].…”

Section: Introductionmentioning

confidence: 99%

Modulating effects of humic acids on genotoxicity induced by water disinfectants in Cyprinus carpio

Gustavino

Buschini

Monfrinotti

et al. 2005

Mutation Research/Genetic Toxicology and Environmental Mutagene

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The use of chlorinated disinfectants during drinking-water production has been shown to generate halogenated compounds as a result of interactions of humic acids with chlorine. Such chlorinated by-products have been shown to induce genotoxic effects and consumption of chlorinated drinking-water has been correlated with increased risk for cancer induction in human populations. The aim of this work was to test the potential genotoxic effects on circulating erythrocytes of the fish Cyprinus carpio exposed in vivo to well-waters disinfected with sodium hypochlorite (NaClO), chlorine dioxide (ClO 2 ) or peracetic acid (CH 3 COO 2 H, PAA), in the absence or presence of standard humic acids (HA). The effects were measured by use of the micronucleus (MN) and the single-cell gel electrophoresis (Comet) assays at different sampling times after a 3-day exposure period. The exposure to chlorine disinfectants without the addition of HA produced a clear toxic effect. Significant cytogenetic damage (i.e. MN induction) was detected in fish populations exposed to both NaClO and ClO 2 with humic acids. In the Comet assay, a significant decrease of DNA migration was observed in erythrocytes of specimens after exposure to NaClO-disinfected water without HA. No effects were observed in any other experimental condition.

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A continuous cell line from the cultured marine fish gilt-head seabream (Sparus aurata L.)

Cited by 117 publications

References 13 publications

Copper induces Cu-ATPase ATP7A mRNA in a fish cell line, SAF1

Copper induces Cu-ATPase ATP7A mRNA in a fish cell line, SAF1

Development and characterization of two cell lines PDF and PDH from Puntius denisonii (Day 1865)

Modulating effects of humic acids on genotoxicity induced by water disinfectants in Cyprinus carpio

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