A consonant model of the tRNA-ribosome complex during the elongation cycle of translation

Mapping the central fold of tRNA2fMet in the P site of the Escherichia coli ribosome

“…As nucleosides 17 or 17.1 and 47 are located on opposite sides of the tRNA molecule, the cross-linking pattern indicates that the P site tRNA is sandwiched between the two ribosomal subunits, with the D loop side facing the SOS particle and the T loop side facing the 30S particle (Figure 11). This interpretation is in agreement with our earlier models of the tRNA-ribosome complex (Wower et al, 1989(Wower et al, , 1991(Wower et al, , 1993 and is, of course, based on the assumption that the tRNA retains a conformation close to or identical with that determined by X-ray diffraction (Kim et al, 1974). All but five of the 77 bases composing E. coli tRNA™'* are engaged in stacking interactions (Woo et al, 1980).…”

Mapping the central fold of tRNA2fMet in the P site of the Escherichia coli ribosome

“…As nucleosides 17 or 17.1 and 47 are located on opposite sides of the tRNA molecule, the cross-linking pattern indicates that the P site tRNA is sandwiched between the two ribosomal subunits, with the D loop side facing the SOS particle and the T loop side facing the 30S particle (Figure 11). This interpretation is in agreement with our earlier models of the tRNA-ribosome complex (Wower et al, 1989(Wower et al, , 1991(Wower et al, , 1993 and is, of course, based on the assumption that the tRNA retains a conformation close to or identical with that determined by X-ray diffraction (Kim et al, 1974). All but five of the 77 bases composing E. coli tRNA™'* are engaged in stacking interactions (Woo et al, 1980).…”

Topography of the E site on the Escherichia coli ribosome.

“…Because azidonucleotides yield very short cross-links, on the order of 2-4 A, upon irradiation, they provide precise information on the molecular environment of the photoreactive base. We have already used azidoadenosine-containing tRNAs to study the topography of the A and P sites on the E. coli ribosome and, on the basis of these data, we have advanced a model of the tRNA -ribosome complex during polypeptide chain elongation (Wower and Zimmermann, 1991). The relative arrangement of A and P site-bound tRNA proposed in the model is consistent with the orientation of mRNA on the 30S subunit and the location of the binding sites for elongation factors on the 5OS subunit.…”

How are tRNAs and mRNA arranged in the ribosome? An attempt to correlate the stereochemistry of the tRNA-mRNA interaction with constraints imposed by the ribosomal topography

“…In the alternative proposal (Figure 24 of the publication) the CC-A-termini reach the 50S subunit by leading around the solvent side of the 30S subunit in a manner similar to that proposed by Spirin (17) (see above). The more recent publications (8,19,78) propose essentially the arrangement of Figure 4b, with the elbows of the tRNAs pointing up and to the left (towards the 30S subunit).…”

Mapping the central fold of tRNA2fMet in the P site of the Escherichia coli ribosome

“…As nucleosides 17 or 17.1 and 47 are located on opposite sides of the tRNA molecule, the cross-linking pattern indicates that the P site tRNA is sandwiched between the two ribosomal subunits, with the D loop side facing the SOS particle and the T loop side facing the 30S particle (Figure 11). This interpretation is in agreement with our earlier models of the tRNA-ribosome complex (Wower et al, 1989(Wower et al, , 1991(Wower et al, , 1993 and is, of course, based on the assumption that the tRNA retains a conformation close to or identical with that determined by X-ray diffraction (Kim et al, 1974). All but five of the 77 bases composing E. coli tRNA™'* are engaged in stacking interactions (Woo et al, 1980).…”

Topography of the E site on the Escherichia coli ribosome.

“…Because azidonucleotides yield very short cross-links, on the order of 2-4 A, upon irradiation, they provide precise information on the molecular environment of the photoreactive base. We have already used azidoadenosine-containing tRNAs to study the topography of the A and P sites on the E. coli ribosome and, on the basis of these data, we have advanced a model of the tRNA -ribosome complex during polypeptide chain elongation (Wower and Zimmermann, 1991). The relative arrangement of A and P site-bound tRNA proposed in the model is consistent with the orientation of mRNA on the 30S subunit and the location of the binding sites for elongation factors on the 5OS subunit.…”

How are tRNAs and mRNA arranged in the ribosome? An attempt to correlate the stereochemistry of the tRNA-mRNA interaction with constraints imposed by the ribosomal topography

“…In the alternative proposal (Figure 24 of the publication) the CC-A-termini reach the 50S subunit by leading around the solvent side of the 30S subunit in a manner similar to that proposed by Spirin (17) (see above). The more recent publications (8,19,78) propose essentially the arrangement of Figure 4b, with the elbows of the tRNAs pointing up and to the left (towards the 30S subunit).…”

Mapping the central fold of tRNA2fMet in the P site of the Escherichia coli ribosome

“…As nucleosides 17 or 17.1 and 47 are located on opposite sides of the tRNA molecule, the cross-linking pattern indicates that the P site tRNA is sandwiched between the two ribosomal subunits, with the D loop side facing the SOS particle and the T loop side facing the 30S particle (Figure 11). This interpretation is in agreement with our earlier models of the tRNA-ribosome complex (Wower et al, 1989(Wower et al, , 1991(Wower et al, , 1993 and is, of course, based on the assumption that the tRNA retains a conformation close to or identical with that determined by X-ray diffraction (Kim et al, 1974). All but five of the 77 bases composing E. coli tRNA™'* are engaged in stacking interactions (Woo et al, 1980).…”

Topography of the E site on the Escherichia coli ribosome.

“…Because azidonucleotides yield very short cross-links, on the order of 2-4 A, upon irradiation, they provide precise information on the molecular environment of the photoreactive base. We have already used azidoadenosine-containing tRNAs to study the topography of the A and P sites on the E. coli ribosome and, on the basis of these data, we have advanced a model of the tRNA -ribosome complex during polypeptide chain elongation (Wower and Zimmermann, 1991). The relative arrangement of A and P site-bound tRNA proposed in the model is consistent with the orientation of mRNA on the 30S subunit and the location of the binding sites for elongation factors on the 5OS subunit.…”

How are tRNAs and mRNA arranged in the ribosome? An attempt to correlate the stereochemistry of the tRNA-mRNA interaction with constraints imposed by the ribosomal topography

“…In the alternative proposal (Figure 24 of the publication) the CC-A-termini reach the 50S subunit by leading around the solvent side of the 30S subunit in a manner similar to that proposed by Spirin (17) (see above). The more recent publications (8,19,78) propose essentially the arrangement of Figure 4b, with the elbows of the tRNAs pointing up and to the left (towards the 30S subunit).…”