A conditioning lesion enhances sympathetic neurite outgrowth

Shoemaker, S.E.; Sachs, H. Hyatt; Vaccariello, Stacey A.; Zigmond, Richard E.

doi:10.1016/j.expneurol.2005.02.023

Cited by 23 publications

(39 citation statements)

References 39 publications

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“…In neurons from the auditory cortex of mammals, LIF is a more potent promoter of neurite outgrowth than BDNF [77]. LIF has also been implicated in peripheral nerve regeneration [12] and is induced by axotomy [78] and other conditioning injuries in vivo [79]. In spite of ample evidence implicating LIF in axonal growth in mice, little is known about LIF's effects on neurite extension in human neurons or in hESC-derived neurons.…”

Section: Lif Promotes Neurite Extension In Hesc-derived Np Cellsmentioning

confidence: 99%

“…This implies that a certain proportion of cells counted as NP cells could be early neurons. HuC/D is a RNA binding protein expressed in the cell bodies of early postmitotic neurons [66][67][68][69][70][71][72][73][74][75][76][77][78], and MAP2 marks dendrites of differentiated neurons [69] (Fig. 4).…”

Section: Lif Increased Proliferation Stat3 Signaling and Yield Of Dmentioning

confidence: 99%

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Neurotrophic Effects of Leukemia Inhibitory Factor on Neural Cells Derived from Human Embryonic Stem Cells

et al. 2012

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Various growth factor cocktails have been used to proliferate and then differentiate human neural progenitor (NP) cells derived from embryonic stem cells (ESC) for in vitro and in vivo studies. However, the cytokine leukemia inhibitory factor (LIF) has been largely overlooked. Here, we demonstrate that LIF significantly enhanced in vitro survival and promoted differentiation of human ESC-derived NP cells. In NP cells, as well as NP-derived neurons, LIF reduced caspase-mediated apoptosis and reduced both spontaneous and H 2 O 2 -induced reactive oxygen species in culture. In vitro, NP cell proliferation and the yield of differentiated neurons were significantly higher in the presence of LIF. In NP cells, LIF enhanced cMyc phosphorylation, commonly associated with self-renewal/proliferation. Also, in differentiating NP cells LIF activated the phosphoinositide 3-kinase and signal transducer and activator of transcription 3 pathways, associated with cell survival and reduced apoptosis. When differentiated in LIF 1 media, neurite outgrowth and ERK1/2 phosphorylation were potentiated together with increased expression of gp130, a component of the LIF receptor complex. NP cells, pretreated in vitro with LIF, were effective in reducing infarct volume in a model of focal ischemic stroke but LIF did not lead to significantly improved initial NP cell survival over nontreated NP cells. Our results show that LIF signaling significantly promotes human NP cell proliferation, survival, and differentiation in vitro. Activated LIF signaling should be considered in cell culture expansion systems for future human NP cell-based therapeutic transplant studies.

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Section: Lif Promotes Neurite Extension In Hesc-derived Np Cellsmentioning

confidence: 99%

Section: Lif Increased Proliferation Stat3 Signaling and Yield Of Dmentioning

confidence: 99%

Neurotrophic Effects of Leukemia Inhibitory Factor on Neural Cells Derived from Human Embryonic Stem Cells

et al. 2012

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“…We recently demonstrated that like sensory and motor neurons, sympathetic neurons exhibit an increase in their rate of regeneration after a conditioning lesion (Shoemaker et al, 2005a). This effect was seen both following a lesion proximal to the neurons' cell bodies and following a distal lesion produced by target organ removal.…”

Section: Introductionmentioning

confidence: 97%

Reduction in nerve growth factor availability leads to a conditioning lesion‐like effect in sympathetic neurons

et al. 2006

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Axotomized peripheral neurons are capable of regeneration, and the rate of regeneration can be enhanced by a conditioning lesion (i.e., a lesion prior to the lesion after which neurite outgrowth is measured). A possible signal that could trigger the conditioning lesion effect is the reduction in availability of a target-derived factor resulting from the disconnection of a neuron from its target tissue. We tested this hypothesis with respect to nerve growth factor (NGF) and sympathetic neurons by administering an antiserum to NGF to adult mice for 7 days prior to explantation or dissociation of the superior cervical ganglion (SCG) and subsequently measuring neurite outgrowth. The antiserum treatment dramatically lowered the concentration of NGF in the SCG and increased the rate of neurite outgrowth in both explants and cell cultures. The increase in neurite outgrowth was similar in magnitude to that seen after a conditioning lesion. To determine if exogenous NGF could block the effect of a conditioning lesion, mice were injected with NGF or cytochrome C immediately prior to unilateral axotomy of the SCG, and for 7 days thereafter. A conditioning lesion effect of similar magnitude was seen in NGF-treated and control animals. While NGF treatment increased NGF levels in the contralateral control ganglion, it did not significantly elevate levels in the axotomized ganglion. The results suggest that the decreased availability of NGF after axotomy is a sufficient stimulus to induce the conditioning lesion effect in sympathetic neurons. While NGF administration did not prevent the conditioning lesion effect, this may be due to the markedly decreased ability of sympathetic neurons to accumulate the growth factor after axotomy.

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“…(Cafferty et al, 2001;Cafferty et al, 2004;Liu and Snider 2001;Qiu et al, 2005). To search the mechanism of conditioning lesion effect, one week after the carotid nerve axotomy superior cervical ganglia were removed and done explant or dissociated cell culture (Shoemaker et al, 2005;Sachs et al, 2010). All these studies need an in vivo step which primarily provide the chance of an in vitro research only after the lesion which is applied on sciatic nerve in vivo.…”

Section: Discussionmentioning

confidence: 99%

Determining of Apoptosis at in vitro Conditioned DRG Neurons with TUNEL Method

Oğuz

2017

Bitlis Eren University Journal of Science and Technology

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There is an increased axonal regeneration after a peripheral nerve is injured if it exposed to an earlier prior injury. The first injury causing the enhanced regeneration is called a conditioning lesion. In this study, Dorsal Root Ganglion primer cell culture was performed then neurons were conditioned in vitro with an effective and new method. It was observed that in vitro conditioned cells were regenerated much faster than control group cells but at the end of 48 th hour, they died. The cell death way of these in vitro conditioned neurons was determined by an in situ Cell Detection Kit Fluorescein and visualized by fluorescent microscopy. Finally, apoptotic cell deaths observed at in vitro conditioned neurons. This study is important for the continuity of enhanced regeneration capacity of conditioning lesion effect and neuronal survival and also it will provide the basis for new studies.

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A conditioning lesion enhances sympathetic neurite outgrowth

Cited by 23 publications

References 39 publications

Neurotrophic Effects of Leukemia Inhibitory Factor on Neural Cells Derived from Human Embryonic Stem Cells

Neurotrophic Effects of Leukemia Inhibitory Factor on Neural Cells Derived from Human Embryonic Stem Cells

Reduction in nerve growth factor availability leads to a conditioning lesion‐like effect in sympathetic neurons

Determining of Apoptosis at in vitro Conditioned DRG Neurons with TUNEL Method

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