A Comprehensive Study on Antioxidant Properties of Crude Extracts from Fruits of Berberis vulgaris L., Cornus mas L. and Mahonia aquifolimum Nutt.

Abstract

The antioxidant capacity of methanolic crude extracts of Berberis vulgaris L., Cornus mas L. and Mahonia aquifolium Nutt. was tested with the thiobarbituric acid reactive substances formation assay, the ferric reducing power (FRAP) and 2,2-diphenyl-2-picrylhydrazyl (DPPH • ) radical scavenging assay. The content of antioxidant components in the extracts, their partition coeffi cient on 1-octanol:water and affi nity to liposome membranes were determined as well. The results show that the IC 50 parameter connected with the antioxidant activity on phosphatidylcholine liposome membrane decreased as follows: B. vulgaris (0.14±0.01 mg/mL) > M. aquifolium (0.34±0.03 mg/mL) > C. mas (1.13±0.01 mg/mL) for AAPH-induced oxidation and M. aquifolium (0.29±0.03 mg/mL) > C. mas (1.24±0.07 mg/mL) > B. vulgaris (1.50±0.05 mg/mL) for Fe(II)/ascorbic acid-induced oxidation, and M. aquifolium (2.35±0.10 mg/mL) > B. vulgaris (2.69±0.04 mg/mL) > C. mas (6.17±0.06 mg/mL) for UVC irradiation. All the extracts exhibited the ability to quench DPPH• and to reduce Fe(III) ions to Fe(II) via redox reaction. The content of active components in the extracts, the partition coeffi cient and extracts affi nity to membranes correlated well with their antioxidant activities.This study has shown that fruits of B. vulgaris, M. aquifolium and C. mas, from which the extracts were obtained, are attractive for consumption and can potentially be used in production of new processed fruit.

Phenol composition, DPPH radical scavenging and antimicrobial activity of cornelian cherry (Cornus mas) fruit and leaf extracts

“…Few data exist about phenol acid, flavonol and anthocyanin composition [6,14,15] and there are no studies about flavan-3-ol composition in Cornelian cherry fruits except in previously described study for the acidified methanol extracts [9]. The (+)-catechin was predominant falavan-3-ol in fruit extracts, followed by (-)-epicatechin and procyanidin B2, while in leaf extracts (-)-epicatechin was predominant and procyanidin B2 was not found.…”

“…Ellagic acid was predominant phenol acid in all extracts, followed by chlorogenic and gallic acids. Others also reported the presence of gallic and ellagic acids in cornelian cherry fruit [6].…”

“…Flavonol compounds found in tested extracts were quercetin-3-glucoside, rutin, quercetin-3-galactoside, luteolin-3-glucoside and kaempferol-3-glucoside. Some authors reported the presence of quercetin and kaempferol derivates in Cornelian cherry fruit extracts [6,14]. The kaempferol-3--glucoside and rutin were predominant flavonols in fruit extracts while quercetin-3-glucoside was less abundant and luteolin-3-glucoside was not detected.…”

“…There have been many studies on antioxidant [4--17], anti-cancer, anti-inflammatory [18], antimicrobial activities [1,4,5,9] of berry fruit extracts which are rich in phenol content [1][2][3][4][5][6][7][8][9][10][11][12][13]. However, there is less research on antioxidant activity and phenol content of Cornelian cherry fruit [6,9,14,15] and leaf extracts [16,17] . Antimicrobial activity of Cornelian cherry fruit was partially described [9,18,19] and there are no research papers on Cornelian cherry leaf extracts.…”

Cornus mas and Cornus Officinalis—Analogies and Differences of Two Medicinal Plants Traditionally Used

“…Thus, both antioxidants content and antioxidant capacity of dogwoods fruits are factors determining the quality of cultivars and basic physicochemical properties. In vitro assays such as free radical scavenging with DPPH (2,2-diphenyl-1-picrylhydrazyl radical), ferric ion reducing antioxidant power (FRAP), total antioxidant capacity with ABTS + (2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation) and oxygen radical absorbance capacity (ORAC) were the most frequently used methods in a wide range of studies on C. mas (Dragović-Uzelac et al, 2007; Pantelidis et al, 2007; Hamid et al, 2011; Celep et al, 2012; Popović et al, 2012; Gunduz et al, 2013; Pyrkosz-Biardzka et al, 2014; Stankovic et al, 2014; Milenković-Andjelković et al, 2015; Hosu et al, 2016; Moldovan et al, 2016) and C. officinalis (Lee et al, 2006; West et al, 2012a; Hwang et al, 2016). Moreover, the hydromethanolic extracts of some cornelian cherry fruit cultivars from Turkey demonstrated significant H 2 O 2 scavenging activity (79.1%; Ersoy et al, 2011).…”