A competitive enzyme-linked immunoassay for domoic acid determination in human body fluids

Enzyme Immunoassay for the Determination of Domoic Acid in Mussel Extracts

“…Finally, although the anti-OVA serum does not crossreact with kainic acid (Smith and Kitts, 1994), the…”

“…presence of other uncharacterized cross-reacting analogues in some mussel samples remains a possibility, albeit a remote one. Saxitoxin also does not cross-react with the anti-OVA-DA serum (Smith and Kitts, 1994); therefore, it is highly unlikely that paralytic shellfish toxins interfered with the domoic acid ELISA.…”

Enzyme Immunoassay for the Determination of Domoic Acid in Mussel Extracts

“…Finally, although the anti-OVA serum does not crossreact with kainic acid (Smith and Kitts, 1994), the…”

“…presence of other uncharacterized cross-reacting analogues in some mussel samples remains a possibility, albeit a remote one. Saxitoxin also does not cross-react with the anti-OVA-DA serum (Smith and Kitts, 1994); therefore, it is highly unlikely that paralytic shellfish toxins interfered with the domoic acid ELISA.…”

Development of a Sensitive Enzyme-Linked Immunosorbent Assay for the Determination of Domoic Acid in Shellfish

“…To render it immunogenic, it is necessary to conjugate domoic acid to a protein carrier. Several approaches have been used to conjugate domoic acid to protein carriers for immunizing animals (12,14,18). Smith and Kitts (12) first reported the generation of polyclonal antibodies against domoic acid in mice; domoic acid was conjugated to either OVA or KLH via the carbodiimide method by linking the carboxyl groups of domoic acid to the amino groups of carrier proteins.…”

“…Several approaches have been used to conjugate domoic acid to protein carriers for immunizing animals (12,14,18). Smith and Kitts (12) first reported the generation of polyclonal antibodies against domoic acid in mice; domoic acid was conjugated to either OVA or KLH via the carbodiimide method by linking the carboxyl groups of domoic acid to the amino groups of carrier proteins. Their ELISA method based on mouse polyclonal antibodies had a low sensitivity for domoic acid with a detection limit at 500 ppb (13).…”

Current Trends and Challenges for Rapid SMART Diagnostics at Point-of-Site Testing for Marine Toxins

“…As the simplest of the EU regulated toxin groups in relation to the single congener domoic acid to be reported there have been numerous ELISA kits developed for ASP detection [81,[102][103][104][105][106][107][108][109][110][111]. Biosense currently has the only commercial ELISA kit for ASP detection that has been validated in single [112] and multi-laboratory [113] studies to AOAC standards.…”

Enzyme Immunoassay for the Determination of Domoic Acid in Mussel Extracts

“…Finally, although the anti-OVA serum does not crossreact with kainic acid (Smith and Kitts, 1994), the…”

“…presence of other uncharacterized cross-reacting analogues in some mussel samples remains a possibility, albeit a remote one. Saxitoxin also does not cross-react with the anti-OVA-DA serum (Smith and Kitts, 1994); therefore, it is highly unlikely that paralytic shellfish toxins interfered with the domoic acid ELISA.…”

Development of a Sensitive Enzyme-Linked Immunosorbent Assay for the Determination of Domoic Acid in Shellfish

“…To render it immunogenic, it is necessary to conjugate domoic acid to a protein carrier. Several approaches have been used to conjugate domoic acid to protein carriers for immunizing animals (12,14,18). Smith and Kitts (12) first reported the generation of polyclonal antibodies against domoic acid in mice; domoic acid was conjugated to either OVA or KLH via the carbodiimide method by linking the carboxyl groups of domoic acid to the amino groups of carrier proteins.…”

“…Several approaches have been used to conjugate domoic acid to protein carriers for immunizing animals (12,14,18). Smith and Kitts (12) first reported the generation of polyclonal antibodies against domoic acid in mice; domoic acid was conjugated to either OVA or KLH via the carbodiimide method by linking the carboxyl groups of domoic acid to the amino groups of carrier proteins. Their ELISA method based on mouse polyclonal antibodies had a low sensitivity for domoic acid with a detection limit at 500 ppb (13).…”

Current Trends and Challenges for Rapid SMART Diagnostics at Point-of-Site Testing for Marine Toxins

“…As the simplest of the EU regulated toxin groups in relation to the single congener domoic acid to be reported there have been numerous ELISA kits developed for ASP detection [81,[102][103][104][105][106][107][108][109][110][111]. Biosense currently has the only commercial ELISA kit for ASP detection that has been validated in single [112] and multi-laboratory [113] studies to AOAC standards.…”

Enzyme Immunoassay for the Determination of Domoic Acid in Mussel Extracts

“…Finally, although the anti-OVA serum does not crossreact with kainic acid (Smith and Kitts, 1994), the…”

“…presence of other uncharacterized cross-reacting analogues in some mussel samples remains a possibility, albeit a remote one. Saxitoxin also does not cross-react with the anti-OVA-DA serum (Smith and Kitts, 1994); therefore, it is highly unlikely that paralytic shellfish toxins interfered with the domoic acid ELISA.…”

Development of a Sensitive Enzyme-Linked Immunosorbent Assay for the Determination of Domoic Acid in Shellfish

“…To render it immunogenic, it is necessary to conjugate domoic acid to a protein carrier. Several approaches have been used to conjugate domoic acid to protein carriers for immunizing animals (12,14,18). Smith and Kitts (12) first reported the generation of polyclonal antibodies against domoic acid in mice; domoic acid was conjugated to either OVA or KLH via the carbodiimide method by linking the carboxyl groups of domoic acid to the amino groups of carrier proteins.…”

“…Several approaches have been used to conjugate domoic acid to protein carriers for immunizing animals (12,14,18). Smith and Kitts (12) first reported the generation of polyclonal antibodies against domoic acid in mice; domoic acid was conjugated to either OVA or KLH via the carbodiimide method by linking the carboxyl groups of domoic acid to the amino groups of carrier proteins. Their ELISA method based on mouse polyclonal antibodies had a low sensitivity for domoic acid with a detection limit at 500 ppb (13).…”

Current Trends and Challenges for Rapid SMART Diagnostics at Point-of-Site Testing for Marine Toxins

“…As the simplest of the EU regulated toxin groups in relation to the single congener domoic acid to be reported there have been numerous ELISA kits developed for ASP detection [81,[102][103][104][105][106][107][108][109][110][111]. Biosense currently has the only commercial ELISA kit for ASP detection that has been validated in single [112] and multi-laboratory [113] studies to AOAC standards.…”