A comparison of the visking membrane and batch sephadex methods of equilibrium dialysis

Ryan, Michael; Kanhere, Shriniwas R; Baraff, Georgia

doi:10.1016/0022-4731(77)90056-5

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1978

1980

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Cited by 4 publications

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“…Additionally, Ryan et al (27) have directly compared equilibrium dialysis and batch Sephadex techniques and find the two methods comparable.…”

Section: Discussionmentioning

confidence: 98%

Diethylstilbestrol and Estradiol Binding to Serum Albumin and Pregnancy Plasma of Rat and Human*

1979

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The equilibrium binding of diethylstilbestrol (DES) and 17 beta-estradiol (E2) to plasma proteins has been characterized. DES exhibits a 10- to 20-fold greater binding affinity index for bovine serum albumin and rat plasma than E2. As expected, E2 gave high values for binding to plasma from pregnant mice or rats, reflecting the presence of alpha-fetoprotein. DES bound to these samples as it did to bovine albumin and rat plasma. These results suggested that DES ineracts weakly with alpha-fetoprotein. This was verified by Scatchard plots of DES and E2 binding to rat and human pregnancy plasma. High affinity, low capacity binding was demonstrated with E2 but not with DES. The significantly lower binding of DES suggests that increased delivery of DES to the fetus may be at least partially responsible for the transplacental toxicity and carcinogenicity of DES.

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“…Additionally, Ryan et al (27) have directly compared equilibrium dialysis and batch Sephadex techniques and find the two methods comparable.…”

Section: Discussionmentioning

confidence: 98%