A comparison of statistical approaches to the derivation of EC3 values from local lymph node assay dose responses

Ethanol and Diethyl Phthalate: Vehicle Effects in the Local Lymph Node Assay

“…The calculated EC3 values are given in Table 2. The assays conducted with BMHCA utilizing EtOH or DEP as the a The estimated conenctration giving rise to a stimulation index of 3 (EC3) was calculated by linear interpretation of the dose response data for each assay according to Basketter et al (1999b). The lower the EC3 value, the greater the skin sensitization potential.…”

Vehicle effects on skin sensitizing potency of four chemicals: assessment using the local lymph node assay

“…The EC3 values for isoeugenol, cinnamic aldehyde and 3-dimethylaminopropylamine in seven vehicles and for dibromodicyanobutane in four vehicles were derived mathematically by linear interpolation [13] and are shown in Table V. With dibromodicyanobutane in DMSO, PG and EtOH/ddw (50 : 50) it was not possible to increase the application concentrations to the levels necessary to derive EC3 values for reasons of toxicity or poor solubility.…”

Further experience with the local lymph node assay using standard radioactive and nonradioactive cell count measurements

“…Values in bold were associated with an increase in thymidine incorporation of > 3.0 fold and were thus judged to be sensitizing. e Calculated (to two significant figures) as described in Basketter et al (1999) or by extrapolation, when appropriate, as detailed in Ryan et al (2007). EC1.5 and EC3 values extrapolated from experimental data without SIs responses above 1.5 or 3, respectively, are displayed in parenthesis.…”