volume 47, issue 2, P193-197 2014
DOI: 10.1590/0037-8682-0233-2013
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Abstract: ABSTRACT Introduction: Polymerase chain reaction (PCR) may offer an alternative diagnostic option when clinical signs and symptoms suggest visceral leishmaniasis (VL) but microscopic scanning and serological tests provide negative results. PCR using urine is sensitive enough to diagnose human visceral leishmaniasis (VL). However, DNA quality is a crucial factor for successful amplifi cation. Methods: A comparative performance evaluation of DNA extraction methods from the urine of patients with VL using two co…

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