A Comparison of Five Serological Tests for the Diagnosis of Experimental Corynebacterium ovis Infection in Sheep

Shigidi, M.T.A.

doi:10.1016/s0007-1935(17)32938-x

Cited by 18 publications

(8 citation statements)

References 5 publications

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“…On the other hand, this test showed high capability in detecting specific antibodies in the very early steps of infection, as all animals have detectable specific antibodies between 6 and 11 days after infection, earlier therefore than other published results with well known less sensitive tests (synergistic haemolysis inhibition test and bacterial agglutination test) (Kuria et al, 2001). However, as our experimental infection procedure provoked only a sub-clinical infection state, as could be expected and already described (Shigidi, 1979;Ellis et al, 1990), this indirect diagnostic tool showed a lack of specificity, as two out of the three animals without granuloma at slaughter were still positive by ELISA.…”

Section: Discussionmentioning

confidence: 49%

Experimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-γ production, IgG avidity and antigen recognition by Western blotting

Paule

Azevedo

Regis

et al. 2003

Veterinary Immunology and Immunopathology

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Corynebacterium pseudotuberculosis is the cause of caseous lymphadenitis (CLA) in small ruminants, a chronic granulomatous disease that provokes significant zootechnics losses to ovine and goat breeders in northern Brazil. The present work was conducted to analyse aspects of humoral and cellular immune responses after experimental infection. Eight goats were infected intradermally with a single dose of virulent C. pseudotuberculosis strain and specific IgG, interferon-gamma (IFN-gamma) production as well as IgG avidity and antigens pattern recognition dynamics against an excreted-secreted antigen were recorded during 20 weeks. At the end of the follow-up, animals were slaughtered and necropsied. Although no animals showed apparent clinical signs of infection at the end of the trial, IFN-gamma response, even more so than the humoral response, differentiated animals into two groups of high or medium/low response. The time-course of IFN-gamma production presented a short-lived primary response on day 5 after infection of animals of both groups, and a strong and long lasting secondary response starting on day 16 after infection in the high response group. The indirect ELISA used was able to detect a positive antibody titre between 6 and 11 days after infection in the two groups. IgG avidity index oscillated initially between 15 and 45%, and showed approximately 5% units increment during the 20 follow-up weeks. With only one individual exception, the qualitative antigens pattern recognition showed on day 11 after infection remained constant through the experiment. IgG avidity is highly correlated with IgG production, but could not be related with specific immunodominant bands. Both humoral and cellular responses kinetics presented a similar pattern of activation/deactivation but necropsy results suggested that the IFN-gamma test would be a very specific marker of CLA status.

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Section: Discussionmentioning

confidence: 49%

Experimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-γ production, IgG avidity and antigen recognition by Western blotting

Paule

Azevedo

Regis

et al. 2003

Veterinary Immunology and Immunopathology

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“…Several diagnostic methods have been proposed for the diagnosis of CLA, including indirect hemagglutination [102], complement fixation [100], immunodiffusion [103], the synergistic inhibition of hemolysis [35] and PCR [104]. Numerous serological tests have been developed to detect antibodies against C. pseudotuberculosis in small ruminants [105].…”

Section: Serological Diagnosis Of Clamentioning

confidence: 99%

Corynebacterium pseudotuberculosis: Immunological Responses in Animal Models and Zoonotic Potential

Bastos¹

2012

J Clin Cell Immunol

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Corynebacterium pseudotuberculosis is a member of the Corynebacterium, Mycobacterium and Nocardia (CMN) group that comprises species of medical, veterinary and biotechnological interest. This pathogen mainly affects small ruminants, causing caseous lymphadenitis (CLA), but it also infects bovines, equines, pigs, deer, camels and humans, showing its zoonotic relevance. Phospholipase D (PLD) and the toxic lipid cell wall are the two most wellstudied virulence factors of this bacterium. They are responsible, in part, for the establishment of disease in the host. Current knowledge on the immunity induced by C. pseudotuberculosis indicates that the resistance to infection is a complex process involving components of both the non-specific and specific host responses, in which humoral and cellular immune responses are both operative. Despite this knowledge and the importance of the disease, a satisfactory vaccine model for sheep and goats has not been developed. Moreover, a control program that includes an efficient diagnostic method in addition to vaccination is crucial for avoiding the spread of bacteria inside flocks. Further, because of its zoonotic potential, C. pseudotuberculosis infection of animals can contaminate meat and milk, putting consumers at risk. The ability of C. pseudotuberculosis to infect both animals and humans makes studies on prevention and diagnosis of this pathogen important.

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“…Therefore the import regulations for goats, especially from countries where the disease is endemic, should be extended to include examinations relevant to the detection of the disease. In addition to clinical inspections serological techniques may be utilised, of which several have been described (8,17,22,23,29). However, chronically infected cases often escape serological detection.…”

Section: Therapy and Controlmentioning

confidence: 99%

An outbreak of caseous lymphadenitis in dairy goats: First report of the disease in the Netherlands

Schreuder¹,

Laak²,

Griesen³

1986

Veterinary Quarterly

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An outbreak of caseous lymphadenitis in which three herds of goats were involved is described. Corynebacterium pseudotuberculosis was shown to be the causative organism. The outbreak was associated with imported goats. As this first report of C. pseudotuberculosis causing caseous lymphadenitis as a clinical entity in small ruminants in the Netherlands, clinical and epizootiological aspects as well as possible methods of control are discussed.

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A Comparison of Five Serological Tests for the Diagnosis of Experimental Corynebacterium ovis Infection in Sheep

Cited by 18 publications

References 5 publications

Experimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-γ production, IgG avidity and antigen recognition by Western blotting

Experimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-γ production, IgG avidity and antigen recognition by Western blotting

Corynebacterium pseudotuberculosis: Immunological Responses in Animal Models and Zoonotic Potential

An outbreak of caseous lymphadenitis in dairy goats: First report of the disease in the Netherlands

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