A comparison of culture and PCR methods for identifying Propionibacterium acnes in lesions isolated from patients with acne

Abstract

Background/aim: One of the factors that affect the occurrence of acne is the presence of Propionibacterium acnes. The present study was conducted to compare the culture and polymerase chain reaction (PCR) methods for identifying P. acnes in lesions isolated from patients with acne. Materials and methods:To examine the presence of P. acnes, 70 samples of acne lesions were collected. Microbial culture and the PCR molecular technique were used to identify P. acnes.Results: Of the total of 70 samples, 14 cases (20%) were identified as P. acnes positive using microbial culture and 58 cases (82.85%) using PCR. The results obtained showed the lack of a relationship between the frequency of P. acnes and factors such as sex, family history of acne, and history of treatment with either of the techniques examined (i.e. the microbial culture and PCR). In contrast, a significant relationship was observed between the frequency of P. acnes and age with the culture method. Conclusion:Given the limitations in the identification of P. acnes using microbial culture, PCR is proposed as a better method with a higher efficiency.

Comparative Study Thymol Oil and Some Gel Face Washing on Resistance Propionibacterium Acnes

“…Also agree with study in Iraq isolated P.acne [17] .These results for identification P.acne were in agreement with [18,19]. Viteck 2 System was a rapid, sensitive, to distinguish clinical isolates (20).…”

“…that acne prevalence was more in female than male, agree with most cases were in ages group between (15)(16)(17)(18)(19)(20).…”

“…The cases Most were obtain patients between the ages [16][17][18][19][20][21] years old in both sexes, and the distribution of acne among female was higher than that of male (14% and 4%) respectively .…”

Comparative Study Thymol Oiland Some Gel Face Washing on Resistance Propionibacterium A

“…thatacne prevalence was more in female than male, agree with most cases were in ages group between (15)(16)(17)(18)(19)(20).Also agree with study in Iraq isolatedP.acne [17] .These results for identificationP.acnewerein agreement with [18,19]. Viteck 2 System was a rapid, sensitive, to distinguish clinical isolates (20).The result agree with [21] The most using treatment (erythromycin) were anti P. acne.…”

“…The cases Most were obtainpatients between the ages [16][17][18][19][20][21] years old in both sexes, andthe distribution of acne among female was higher than that of male (14% and 4%) respectively . ----------------9.135 ** 9.852 ** ** (P≤0.01)…”