A comparison of bioreactors for culture of fetal mesenchymal stem cells for bone tissue engineering

Zhang, Zhi‐Yong; Teoh, Swee Hin; Teo, Erin Yiling; Chong, Mark Seow Khoon; Shin, Chong Woon; Tien, Foo Toon; Choolani, Mahesh; Chan, Jerry Kok Yen

doi:10.1016/j.biomaterials.2010.07.097

Cited by 90 publications

(66 citation statements)

References 51 publications

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“…Alternatively, bi-axial wavy-walled bioreactors provided modular and more homogeneous shear stress compared to the uniaxial counterpart, which could be a flexible and scalable system for MSC aggregates. 129,130 Evidences were provided that the bioreactors enhanced oxygen and nutrient diffusion inside aggregates (100-300 mm) compared to static cultures. 131,132 The dynamic culture conditions create different oxygen profiles in MSC aggregates, which may affect the cell metabolism and phenotype.…”

Section: D Msc Aggregates: Mechanisms Properties and Applicationsmentioning

confidence: 99%

Three-Dimensional Aggregates of Mesenchymal Stem Cells: Cellular Mechanisms, Biological Properties, and Applications

Sart

Tsai

et al. 2014

Tissue Engineering Part B: Reviews

326

362

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Mesenchymal stem cells (MSCs) are primary candidates in cell therapy and tissue engineering and are being tested in clinical trials for a wide range of diseases. Originally isolated and expanded as plastic adherent cells, MSCs have intriguing properties of in vitro self-assembly into three-dimensional (3D) aggregates reminiscent of skeletal condensation in vivo. Recent studies have shown that MSC 3D aggregation improved a range of biological properties, including multilineage potential, secretion of therapeutic factors, and resistance against ischemic condition. Hence, the formation of 3D MSC aggregates has been explored as a novel strategy to improve cell delivery, functional activation, and in vivo retention to enhance therapeutic outcomes. This article summarizes recent reports of MSC aggregate self-assembly, characterization of biological properties, and their applications in preclinical models. The cellular and molecular mechanisms underlying MSC aggregate formation and functional activation are discussed, and the areas that warrant further investigation are highlighted. These analyses are combined to provide perspectives for identifying the controlling mechanisms and refining the methods of aggregate fabrication and expansion for clinical applications.

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Section: D Msc Aggregates: Mechanisms Properties and Applicationsmentioning

confidence: 99%

Three-Dimensional Aggregates of Mesenchymal Stem Cells: Cellular Mechanisms, Biological Properties, and Applications

Sart

Tsai

et al. 2014

Tissue Engineering Part B: Reviews

326

362

View full text Add to dashboard Cite

show abstract

“…Mechanical stimulation such as longitudinal strain accelerated the bone morphogenesis from MSCs [62] . Zhang et al [63] developed a biaxial rotating bioreactor (BXR) to generate tissue engineered bone grafts from human fetal MSC and showed that higher cellularity, confluence, and more robust osteogenic differentiation were achieved in the BXR as compared to spinner flasks, perfusion and rotating wall bioreactors. Human ligaments were engineered from human MSCs in a bioreactor by combining dynamic tension and torsion mimicking forces in vivo, which significantly improved the ligament function [64] .…”

Section: Mechanical Stimulationmentioning

confidence: 99%

Engineering stem cell niches in bioreactors

Liu

Liu²,

Zang³

et al. 2013

WJSC

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Stem cells, including embryonic stem cells, induced pluripotent stem cells, mesenchymal stem cells and amniotic fluid stem cells have the potential to be expanded and differentiated into various cell types in the body. Efficient differentiation of stem cells with the desired tissue-specific function is critical for stem cell-based cell therapy, tissue engineering, drug discovery and disease modeling. Bioreactors provide a great platform to regulate the stem cell microenvironment, known as "niches", to impact stem cell fate decision. The niche factors include the regulatory factors such as oxygen, extracellular matrix (synthetic and decellularized), paracrine/ autocrine signaling and physical forces (i.e. , mechanical force, electrical force and flow shear). The use of novel bioreactors with precise control and recapitulation of niche factors through modulating reactor operation parameters can enable efficient stem cell expansion and differentiation. Recently, the development of microfluidic devices and microbioreactors also provides powerful tools to manipulate the stem cell microenvironment by adjusting flow rate and cytokine gradients. In general, bioreactor engineering can be used to better modulate stem cell niches critical for stem cell expansion, differentiation and applications as novel cell-based biomedicines. This paper reviews important factors that can be more precisely controlled in bioreactors and their effects on stem cell engineering.

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“…These mechanical forces (local strains and interstitial fluid velocities) (30-31) facilitate cell maturation and osteogenic differentiation (32)(33). Uniform cell distribution is yet another advantage of the perfusion bioreactor in comparison with the static culture since the cell culture in static condition creates inhomogeneous cell distribution (34).…”

Section: Discussionmentioning

confidence: 99%

Osteogenic Differentiation and Mineralization on Compact Multilayer nHA-PCL Electrospun Scaffolds in a Perfusion Bioreactor

et al. 2016

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Background:Monolayer electrospun scaffolds have already been used in bone tissue engineering due to their high surface-tovolume ratio, interconnectivity, similarity to natural bone extracellular matrix (ECM), and simple production. Objectives: The aim of this study was to evaluate the dynamic culture effect on osteogenic differentiation and mineralizationi into a compact cellular multilayer nHA-PCL electrospun construct. The dynamic culture was compared with static culture. Materials and Methods:The calcium content, alkaline phosphatase (ALP) activity and cell viability were investigated on days 3 and 7. Results: When the dynamic culture compared to static culture, the mineralization and ALP activity were increased in dynamic culture. After 7 days, calcium contents were 41.24 and 20.44 μg.(cm 3 ) -1 , and also normalized ALP activity were 0.32 and 0.19 U.mg -1 in dynamic and static culture, respectively. Despite decreasing the cell viability until day 7, the scanning electron microscopy (SEM) results showed that, due to higher mineralization, a larger area of the construct was covered with calcium deposition in dynamic culture. Conclusions:The dynamic flow could improve ALP activity and mineralization into the compact cellular multilayer construct cultured in the perfusion bioreactor after 7 days. Fluid flow of media helped to facilitate the nutrients transportation into the construct and created uniform cellular construct with high mineralization. This construct can be applied for bone tissue engineering.

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A comparison of bioreactors for culture of fetal mesenchymal stem cells for bone tissue engineering

Cited by 90 publications

References 51 publications

Three-Dimensional Aggregates of Mesenchymal Stem Cells: Cellular Mechanisms, Biological Properties, and Applications

Three-Dimensional Aggregates of Mesenchymal Stem Cells: Cellular Mechanisms, Biological Properties, and Applications

Engineering stem cell niches in bioreactors

Osteogenic Differentiation and Mineralization on Compact Multilayer nHA-PCL Electrospun Scaffolds in a Perfusion Bioreactor

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