A comparison between simultaneous saccharification and fermentation and separate hydrolysis and fermentation using steam-pretreated corn stover

Öhgren, Karin; Bura, Renata; Lesnicki, Gary; Saddler, Jack N.; Zacchi, Guido

doi:10.1016/j.procbio.2007.02.003

Cited by 303 publications

(145 citation statements)

References 26 publications

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“…Most microbial β-glucosidases have glucose Ki ranging from as low as 0.5 mM to 100 mM or more [14]. Although glucose acted as a competitive inhibitor of pNPG hydrolysis by TkBgl3A, with a Ki of 3.29 mM, the glucose inhibition of β-glucosidase could be overcome by an SSF process in which glucose is rapidly converted to ethanol by yeast [39]. The purified TkBgl3A was stable under SSF conditions, which facilitates the use of the enzyme in SSF.…”

Section: Discussionmentioning

confidence: 99%

Predominance of Trichoderma and Penicillium in cellulolytic aerobic filamentous fungi from subtropical and tropical forests in China, and their use in finding highly efficient β-glucosidase

Zhang

Junliang

Lan

et al. 2014

Biotechnol Biofuels

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Background: Cellulose is the most abundant biomass on earth. The major players in cellulose degradation in nature are cellulases produced by microorganisms. Aerobic filamentous fungi are the main sources of commercial cellulase. Trichoderma reesei has been explored extensively for cellulase production; however, its major limitations are its low β-glucosidase activity and inefficiency in biomass degradation. The aim of this work was to isolate new fungal strains from subtropical and tropical forests in China, which produce high levels of cellulase in order to facilitate development of improved commercial cellulases.

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Section: Discussionmentioning

confidence: 99%

Predominance of Trichoderma and Penicillium in cellulolytic aerobic filamentous fungi from subtropical and tropical forests in China, and their use in finding highly efficient β-glucosidase

Zhang

Junliang

Lan

et al. 2014

Biotechnol Biofuels

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“…In this report, enzymatic hydrolysis and fermentation have been conducted separately to facilitate understanding of the individual processes. Nevertheless, Simultaneous Saccharification and Fermentation (SSF) was shown to be beneficial compared to Separate Hydrolysis and Fermentation (SHF) (20), despite a lower reaction temperature that was adopted to allow fermentation at the expense of greater efficiency during enzymatic hydrolysis. The full potential of these configurations will be better realized if both hydrolysis and fermentation share common optimal conditions.…”

Section: Discussionmentioning

confidence: 99%

Cellulosic ethanol production from AFEX-treated corn stover using Saccharomyces cerevisiae 424A(LNH-ST)

Lau

Dale²

2009

Proc. Natl. Acad. Sci. U.S.A.

328

200

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Current technology using corn stover (CS) as feedstock, Ammonia Fiber Expansion (AFEX) as the pretreatment technology, and Saccharomyces cerevisiae 424A(LNH-ST) as the ethanologenic strain in Separate Hydrolysis and Fermentation was able to achieve 191.5 g EtOH/kg untreated CS, at an ethanol concentration of 40.0 g/L (5.1 vol/vol%) without washing of pretreated biomass, detoxification, or nutrient supplementation. Enzymatic hydrolysis at high solids loading was identified as the primary bottleneck affecting overall ethanol yield and titer. Degradation compounds in AFEX-pretreated biomass were shown to increase metabolic yield and specific ethanol production while decreasing the cell biomass generation. Nutrients inherently present in CS and those resulting from biomass processing are sufficient to support microbial growth during fermentation. This platform offers the potential to improve the economics of cellulosic ethanol production by reducing the costs associated with raw materials, process water, and capital equipment.AFEX ͉ fermentation ͉ Lignocellulose ͉ biofuel

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“…Organic waste, primarily lignocellulose materials, is being generated in ever larger quantities, particularly by the food industry (1). To address environmental concerns over organic waste disposal, it is necessary to find cost-effective uses for these by-products.…”

Section: Introductionmentioning

confidence: 99%

“…Increasingly, lignocellulosic biomass has been used as a substrate in biotechnological processes, mainly for the production of bio-fuels and organic acids. The principal components of lignocellulosic materials are cellulose, hemicellulose and lignin (1).…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Conversion of Sugar Beet Pulp: A Comparison of Simultaneous Saccharification and Fermentation and Separate Hydrolysis and Fermentation for Lactic Acid Production

Berłowska

Cieciura-Włoch

Kalinowska

et al. 2018

Food Technol. Biotechnol.

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SummaryThis study compares the efficiency of lactic acid production by separate hydrolysis and fermentation (SHF) or simultaneous saccharification and fermentation (SSF) of sugar beet pulp (SBP), a by-product of industrial sugar production. In experiments, SBP was hydrolyzed using 5 commercial enzymes. A series of shake flask scale fermentations and was conducted using 5 selected strains of lactic acid bacteria (LAB). The differences in the activities of the enzymes for degrading the principal SBP components were reflected in the different yields of total reducing sugars. The highest yields from hydrolysis and the lowest quantities of insoluble residues were obtained using a mixture (1:1) of Viscozyme and Ultraflo Max. In the SHF process, only a portion of the soluble sugars released by the enzymes from the SBP was assimilated by the LAB strains. In SSF, low enzyme loads revealed reductions in the efficiency of sugar accumulation. The risk of carbon catabolic repression was reduced. Our results suggest www.ftb.com.hrPlease note that this is an unedited version of the manuscript that has been accepted for publication. This version will undergo copyediting and typesetting before its final form for publication. We are providing this version as a service to our readers. The published version will differ from this one as a result of linguistic and technical corrections and layout editing. that SSF has advantages over SHF, including lower processing costs and higher productivity.Lactic acid yield in SSF mode (around 30 g/L) was 80-90 % higher than that for SHF.

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A comparison between simultaneous saccharification and fermentation and separate hydrolysis and fermentation using steam-pretreated corn stover

Cited by 303 publications

References 26 publications

Predominance of Trichoderma and Penicillium in cellulolytic aerobic filamentous fungi from subtropical and tropical forests in China, and their use in finding highly efficient β-glucosidase

Predominance of Trichoderma and Penicillium in cellulolytic aerobic filamentous fungi from subtropical and tropical forests in China, and their use in finding highly efficient β-glucosidase

Cellulosic ethanol production from AFEX-treated corn stover using Saccharomyces cerevisiae 424A(LNH-ST)

Enzymatic Conversion of Sugar Beet Pulp: A Comparison of Simultaneous Saccharification and Fermentation and Separate Hydrolysis and Fermentation for Lactic Acid Production

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