A Comparison between Inbred and Hybrid Maize Haploid Inducers

Trentin, Henrique Uliana; Yavuz, Recep; Dermail, Abil; Frei, Ursula; Dutta, Somak; Lübberstedt, Thomas

doi:10.3390/plants12051095

Cited by 5 publications

(4 citation statements)

References 29 publications

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“…We recommend further phenotypic evaluations in inducer families with qhir1+ / qhir8+ genotypes, focusing on traits related to the ideotype of haploid inducers, such as plant height, ear height, flowering behaviors, tassel and pollen attributes, and seed set. This assessment will help determine the feasibility of those genotypes in haploid induction stage, whether in induction nurseries or isolation fields ( Trentin et al., 2020 ; Trentin et al., 2023b ). Considering the polygenic nature governing HIR and those mentioned agronomic traits, genomic selection approach can be applied to simultaneously identify promising parents to generate progenies with favorable performance on targeted traits prior to field evaluation.…”

Section: Discussionmentioning

confidence: 99%

Accelerating haploid induction rate and haploid validation through marker-assisted selection for qhir1 and qhir8 in maize

Khammona,

Dermail,

Suriharn

et al. 2024

Front. Plant Sci.

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Doubled haploid (DH) technology becomes more routinely applied in maize hybrid breeding. However, some issues in haploid induction and identification persist, requiring resolution to optimize DH production. Our objective was to implement simultaneous marker-assisted selection (MAS) for qhir1 (MTL/ZmPLA1/NLD) and qhir8 (ZmDMP) using TaqMan assay in F2 generation of four BHI306-derived tropical × temperate inducer families. We also aimed to assess their haploid induction rate (HIR) in the F3 generation as a phenotypic response to MAS. We highlighted remarkable increases in HIR of each inducer family. Genotypes carrying qhir1 and qhir8 exhibited 1 – 3-fold higher haploid frequency than those carrying only qhir1. Additionally, the qhir1 marker was employed for verifying putative haploid seedlings at 7 days after planting. Flow cytometric analysis served as the gold standard test to assess the accuracy of the R1-nj and the qhir1 marker. The qhir1 marker showed high accuracy and may be integrated in multiple haploid identifications at early seedling stage succeeding pre-haploid sorting via R1-nj marker.

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Section: Discussionmentioning

confidence: 99%

Accelerating haploid induction rate and haploid validation through marker-assisted selection for qhir1 and qhir8 in maize

Khammona,

Dermail,

Suriharn

et al. 2024

Front. Plant Sci.

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“…Each inducer and donor block was composed of subblocks containing 16 plots. Inducers were not randomized among subblocks because the great difference in vigor among them would adversely affect other traits for which data were collected for a companion study ( Trentin et al., 2023 ). This rationale also justified sowing closely related inducers side-by-side within each subblock.…”

Section: Methodsmentioning

confidence: 99%

Genetic basis of maize maternal haploid induction beyond MATRILINEAL and ZmDMP

Trentin,

Krause,

Zunjare

et al. 2023

Front. Plant Sci.

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In maize, doubled haploid (DH) lines are created in vivo through crosses with maternal haploid inducers. Their induction ability, usually expressed as haploid induction rate (HIR), is known to be under polygenic control. Although two major genes (MTL and ZmDMP) affecting this trait were recently described, many others remain unknown. To identify them, we designed and performed a SNP based (~9007) genome-wide association study using a large and diverse panel of 159 maternal haploid inducers. Our analyses identified a major gene near MTL, which is present in all inducers and necessary to disrupt haploid induction. We also found a significant quantitative trait loci (QTL) on chromosome 10 using a case-control mapping approach, in which 793 noninducers were used as controls. This QTL harbors a kokopelli ortholog, whose role in maternal haploid induction was recently described in Arabidopsis. QTL with smaller effects were identified on six of the ten maize chromosomes, confirming the polygenic nature of this trait. These QTL could be incorporated into inducer breeding programs through marker-assisted selection approaches. Further improving HIR is important to reduce the cost of DH line production.

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“…Marker-assisted selection on two loci mentioned above among F2 individuals may fasten the selection gain since the targeted trait, HIR, is controlled by recessive alleles. Hybrid inducers are an alternative solution to enhance the efficiency of haploid induction under isolation fields by reducing the workload but still retaining the ability to produce haploids (Dermail et al 2023;Trentin et al 2023) Improving the effectiveness of the haploid identification system is also imperative; otherwise, the HIR obtained will not be reliable. The use of reduced seedling vigor was effective in detecting false positives among putative haploids in tropical backgrounds.…”

Section: Improving Haploid Induction Rate and The Reliability Of Hapl...mentioning

confidence: 99%

Stratified haploid identification system through the R1-nj kernel and reduced seedling vigor in tropical maize germplasm

THAWARORIT,

DERMAIL,

LERTRAT

et al. 2023

Biodiversitas

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Abstract. Thawarorit A, Dermail A, Lertrat K, Chankaew S, Suriharn K. 2023. Stratified haploid identification system through the R1-nj kernel and reduced seedling vigor in tropical maize germplasm. Biodiversitas 24: 4262-4268. Haploid identification through the R1-nj marker is commonly applied in in-vivo doubled haploid technology in maize, although misclassification issues often occur. We explored the innate properties of maternal haploids at the seedling stage to verify true haploids and to reduce false positives. There are many questions about whether reduced seedling vigor is effective for haploid verification in tropical maize backgrounds. This study aimed to evaluate the haploid induction rate of Stock6-derived F3 population inducers and to investigate the effectiveness of reduced seedling vigor at the V2/V3stage as an alternative marker to verify putative haploids. Field trials and haploid induction were conducted in the rainy season of 2021 and the dry season of 2021/22 in Khon Kaen, Thailand. Two tropical source germplasm: P789 and S7328, were included as donor females. Haploids were identified through the R1-nj marker and reduced seedling vigor. Significant reduction of haploid frequency up to 2.5% on average between the two identification methods, indicating that a considerable number of false positives could be found using reduced seedling vigor. Inducer population K11 was promising since it performed a stable ability to produce haploids over donors and seasons and had a low misclassification rate. Further breeding strategies and haploid selection schemes are discussed.

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A Comparison between Inbred and Hybrid Maize Haploid Inducers

Cited by 5 publications

References 29 publications

Accelerating haploid induction rate and haploid validation through marker-assisted selection for qhir1 and qhir8 in maize

Accelerating haploid induction rate and haploid validation through marker-assisted selection for qhir1 and qhir8 in maize

Genetic basis of maize maternal haploid induction beyond MATRILINEAL and ZmDMP

Stratified haploid identification system through the R1-nj kernel and reduced seedling vigor in tropical maize germplasm

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