2014
DOI: 10.1007/s10616-014-9825-x View full text |Buy / Rent full text
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Abstract: Cytokine-induced killer (CIK) cells and T cells expanded by co-stimulation with beads presenting anti-CD3 and -CD28 antibodies are both polyclonal T cells under intensive laboratory and clinical studies, but there has not been any direct comparison between both. We compared the expansion, memory T cell subsets and cytotoxicity for T cells expanded in parallel by the two methods. Bead-stimulated T cells showed superior expansion as compared to CIK cells on D14 of culture. Bead-stimulated T cells consisted of a … Show more

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“…Allogeneic protocols were reported by Iudicone et al [29] and Guo et al [30]. Autologous protocols were also reported by Niam et al [31], Liu et al [32], and Chan and Linn [33], who have obtained CIK cells from patients with myeloid leukemia, hepatocellular carcinoma, and polycythemia, respectively. According to Zhang et al [34], the antitumor efficacy of autologous CIK cells derived from cancer patients was lower because of the immunosuppressive state of patients, compared with the allogeneic CIK cells obtained from adult healthy individuals.…”
Section: Discussionmentioning
“…On the other hand, these findings were not in line with Bonanno et al [46], Niam et al [31], and Wei et al [38], who cultured human PBMCs for 21, 28, and 15 days, respectively, to reach the maturation stage. In addition, Chan and Linn [33] cultured human PBMCs for 26 days and they started counting and evaluating morphological and phenotypical changes of CIK cells on day 10 as they assumed that it is too early to assess cultured CIK cells before day 10.…”
Section: Discussionmentioning
“…Highaffinity TCRs may allow CATs to respond to target cells bearing a low level of cognate peptide. A previous report also compared the activities of CATs and T cells stimulated with anti-CD3/CD28 microbeads stimulated against myelomonocytic leukemia [34] and demonstrated that CATs had superior cytotoxicity. We compared the activities of TCR-CAT and TCR-T against melanoma tumor cells.…”
Section: Discussionmentioning