A comparative study on growth performance and biochemical composition of mixed culture of Isochrysis galbana and Chaetoceros calcitrans with monocultures

Phatarpekar, P. V.; Sreepada, R.A.; Pednekar, Chhaya; Achuthankutty, C.T.

doi:10.1016/s0044-8486(99)00227-6

Cited by 58 publications

(24 citation statements)

References 29 publications

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“…No adaptation phase was detected in either culture. If it did occur, it was very mild, similar to that reported by Phatarpekar et al (2000) for the same species; this could be attributed to faster cellular reproduction than in other species. For example, adaptation phases longer than 1 day have been reported for Chaetoceros muelleri and C. gracilis, Tetraselmis tetrathele and T. gracilis, Dunaliella salina and D. tertiolecta, and Navicula incerta, among others (Calderón & Serpa, 2003;Leal et al, 2003;Affan et al, 2007;MouraJunior et al, 2007).…”

Section: Growth and Cryopreservationsupporting

confidence: 79%

Cryopreservation of the microalgae Chaetoceros calcitrans (Paulsen): analysis of the effect of DMSO temperature and light regime during different equilibrium periods

Leiva

Dupré

2011

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ABSTRACT.We evaluated the effect of three variables (cryoprotectant temperature, light regime, and time of exposure to the cryoprotectant) throughout the equilibrium period during cryopreservation on the viability of the microalga Chaetoceros calcitrans (Paulsen). For this, the cryoprotectant dimethyl sulfoxide (DMSO) at 5% (v/v) was added at three different temperatures (4, 10, and 25ºC) before placing the microalgae in cryobiological straws for freezing. Once inside the cryobiological straws, the microalgal-cryoprotectant suspensions were subjected to the following light regimes for 15 or 45 min: complete light, complete darkness, light/darkness, and darkness/light. Suspensions were then frozen under controlled conditions and stored in liquid nitrogen. The viability index proposed by Cañavate & Lubian (1995b) was used to measure microalgal viability after cryopreservation. Results indicated that it is necessary to use a cryoprotectant to ensure the viable cryopreservation of C. calcitrans. Statistical analyses showed that the temperature of the DMSO influenced the viability of cryopreserved microalgae and that there was no synergistic effect between the other variables studied. The viabilities obtained with DMSO at 25ºC, 10ºC, and 4ºC were 34.9%, 27.8%, and 20.6%, respectively. Keywords: cryopreservation, diatoms, DMSO, temperature, light, equilibrium period.Criopreservación de las microalgas Chaetoceros calcitrans (Paulsen): análisis del efecto de la temperatura de DMSO y régimen de luz durante diferentes períodos de equilibrio RESUMEN. Se evaluó el efecto de tres variables − la temperatura del crioprotector, el régimen de luz y el tiempo de exposición al crioprotector durante el período de equilibrio en el proceso de criopreservación − sobre la viabilidad de la microalga Chaetoceros calcitrans (Paulsen). Para ello se utilizó, el crioprotector dimetilsulfóxido (DMSO) al 5% (v/v), el cual fue adicionado a tres temperaturas diferentes (4, 10 y 25ºC) antes de que las microalgas fueran introducidas dentro de pajuelas criobiológicas para su congelación. Una vez dentro de las pajuelas, las suspensiones de microalgas con crioprotector se sometieron durante 15 o 45 min a luz completa, oscuridad completa, luz/oscuridad y oscuridad/luz. Luego se congelaron de manera controlada y se almacenaron en nitrógeno líquido. La viabilidad de las microalgas posterior a la criopreservación fue medida a través del índice de viabilidad propuesto por Cañavate & Lubian (1995b). Los resultados indicaron que es necesario el uso de criprotector para la criopreservación viable de C. calcitrans. El análisis estadístico demostró que la temperatura del DMSO influye sobre la viabilidad de la microalga criopreservada y que no existe efecto sinérgico entre las demás variables de estudio. Las viabilidades alcanzadas usando DMSO a 25ºC, 10ºC y 4ºC fueron de 34,9%, 27,8% y 20,6% respectivamente.

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Section: Growth and Cryopreservationsupporting

confidence: 79%

Cryopreservation of the microalgae Chaetoceros calcitrans (Paulsen): analysis of the effect of DMSO temperature and light regime during different equilibrium periods

Leiva

Dupré

2011

lajar

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“…The maximum absorbance was achieved at the end of the stationary phase. Previous works reported values of μ: 0.225-0.665 d − 1 and dt: 3.080-1.042 d for Chaetoceros calcitrans (Phatarpekar et al, 2000), and values of μ: 0.33-1.97 d − 1 and dt: 2.100-0.352 d for I. affinis galbana (T-Iso), obtained by optimizing growth conditions (Marchetti et al, 2012). Regarding N. gaditana and P. tricornutum values of μ: 0.2-1.44 d − 1 , dt: 3.46-0.48 d, and of μ: 0.2-1.44 d −1 , dt: 3.46-0.48 d, respectively, have been also reported .…”

Section: Microalgae Maximum Absorbancementioning

confidence: 89%

A simple spectrophotometric method for biomass measurement of important microalgae species in aquaculture

et al. 2015

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“…This effect is caused by the photooxidation reaction inside the cell as the excess light cannot be absorbed into the photosynthetic apparatus. Different species of microalgae respond differently to light intensity and photoperiod Renaud et al 1991;Ma et al 1997;Phatarpekar et al 2002;Danesi et al 2004;Richmond 2004).…”

Section: Discussionmentioning

confidence: 99%

Effect of light intensity and photoperiod on biomass and fatty acid composition of the microalgae, Chlorella vulgaris

2011

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The effects of irradiance and photoperiod on the biomass and fatty acid (FA) composition of Chlorella vulgaris were examined in the exponential growth phase. Results indicated significant differences in biomass and FA at different intensities and photoperiods. Maximum biomass (2.05 ± 0.1 g l -1 ) was at 62.5 lmol photons m -2 s -1 and 16:8 h light/dark photoperiod. FA composition changed considerably in different light regimes; the maximum percentage of total saturated (SFA) (33.38%) was recorded at 100 lmol photons m -2 s -1 and 16:8 h photoperiod, while monounsaturated (MUFA) and polyunsaturated (PUFA) fatty acids decreased with increasing irradiance and light duration. The maximum percentage of total MUFA (15.93%) and PUFA (27.40%) was recorded at 37.5 lmol photons m -2 s -1 and 8:16 h photoperiod.

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A comparative study on growth performance and biochemical composition of mixed culture of Isochrysis galbana and Chaetoceros calcitrans with monocultures

Cited by 58 publications

References 29 publications

Cryopreservation of the microalgae Chaetoceros calcitrans (Paulsen): analysis of the effect of DMSO temperature and light regime during different equilibrium periods

Cryopreservation of the microalgae Chaetoceros calcitrans (Paulsen): analysis of the effect of DMSO temperature and light regime during different equilibrium periods

A simple spectrophotometric method for biomass measurement of important microalgae species in aquaculture

Effect of light intensity and photoperiod on biomass and fatty acid composition of the microalgae, Chlorella vulgaris

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