A comparative study of lymph node mast cell populations in five marsupial species

Chiarini-Garcia, Hélio; Pereira, Fabiano Moraes

doi:10.1054/tice.1999.0049

Cited by 16 publications

(8 citation statements)

References 32 publications

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“…Mast cells, which are regarded as immunoregulatory cells [Metcalfe et al, 1997], have recently been described in marsupials, especially in Didelphis but only for lymph nodes, intestine and skin and not for the genital tract [Haynes, 1991;Santos and Machado, 1994;Chiarini-Garcia and Pereira, 1999;Chiarini-Garcia et al, 2000]. Mast cells staining metachromatically with toluidine blue have been mentioned in the uterus of the marsupial Bettongia by Tyndale-Biscoe [1968].…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural Changes in the Cervical Epithelium during the Estrous Cycle of the Marsupial Monodelphis domestica (Grey Short-Tailed Opossum)

Wick

Kress²

2002

Cells Tissues Organs

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Ultrastructural changes in the cervical epithelium related to the estrous cycle have been studied in the South American marsupial Monodelphis domestica. The two cervices protrude with prominent papillae into the sinus vaginalis. At times of simple columnar at others of more pseudostratified character consists of two types of cells, ciliated and secretory cells. The mucosal epithelium is uniform in its entire length and shows no division into an endo- and ectocervix. The mucosa of the cervix differs from the uterine endometrium. There are no glandular structures, but the luminal epithelium shows deep invaginations underlined by dense connective tissue. The most conspicuous changes include the height and the differentiation of the cervical epithelium which attains its maximum development during estrus, where secretory cells are fully packed with large secretory granules and ciliated cells are well developed. After extruding their granules, secretory cells may transform into ciliated cells, while ciliated cells show the phenomenon of deciliation in which cilia packets are shed into the cervical lumen. This transformation process takes place mainly during post- and metestrus. The presence of solitary cilia is only noticeable during pro-estrus, being in contrast to the uterine epithelium where they appear during the whole estrous cycle.

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Section: Discussionmentioning

confidence: 99%

Ultrastructural Changes in the Cervical Epithelium during the Estrous Cycle of the Marsupial Monodelphis domestica (Grey Short-Tailed Opossum)

Wick

Kress²

2002

Cells Tissues Organs

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“…Mast cells have recently been described in marsupials, especially in Didelphis, but only in lymph nodes, intestine and skin, not in the genital tract [Haynes, 1991;Santos and Machado, 1994;Chiarini-Garcia and Pereira, 1999;Chiarini-Garcia et al, 2000]. Mast cells staining metachromatically with toluidine blue were noted in the uterus of the marsupial Bettongia by Tyndale-Biscoe [1968].…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural Changes in the Uterine Luminal and Glandular Epithelium during the Oestrous Cycle of the Marsupial Monodelphis domestica (Grey Short-Tailed Opossum)

Wick

Kress²

2001

Cells Tissues Organs

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Ultrastructural changes in the endometrium associated with the oestrous cycle were studied in the South American marsupial Monodelphis domestica. The most conspicuous changes include the height and the differentiation of the uterine luminal and glandular epithelium, which consists of ciliated and non-ciliated cells. The glandular epithelium attains its maximum development during oestrus, the luminal epithelium at postoestrus. A distinct increase in the number of ciliated cells can be observed during pro-oestrus, reaching a maximum number at oestrus; this is followed by a process of deciliation. The presence of solitary cilia on the apices of non-ciliated cells is very conspicuous during all oestrous stages and can best be seen on the luminal epithelium. These findings differ from the observations in eutherian mammals, where solitary cilia are only found in the immature uterus or after ovariectomy. The secretory activity of non-ciliated cells of the luminal epithelium is hardly noticeable along the apical membrane and stains only very faintly with Alcian blue. The glandular epithelium cells are filled apically with exocytotic vesicles at oestrus and early postoestrus. However, in contrast to the cervical gland cells, they hardly stain with Alcian blue, indicating that mucins of a different type must be present. Mechanisms for the remodelling of the luminal and glandular epithelium are especially conspicuous at metoestrus and early pro-oestrus and include the presence of autolysosomes, residual bodies and apoptotic bodies. In the endometrial stroma, around the uterine glands, macrophages accumulate and attain a typical oestrous stage-dependent appearance during their phagocytotic activities.

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“…Abundance of mast cells in the lymph nodes has been described in a Dasyurid, the fattailed dunnart (Smithopsis crassicaudata), and in five Didelphidae marsupials. These mast cells were restricted to the lymphatic sinuses, contained large cytoplasmic granules and were thought to be of a different type from the connective tissue and mucosal mast cell populations (Haynes, 1991;Chiarini-Garcia and Pereira, 1999;Chiarini-Garcia et al, 2000). The medullary sinuses of the devils lymph nodes enclosed large numbers of toluidine blue positive cells, morphologically consistent with mast cells.…”

Section: Lymphoid Tissues Of the Tasmanian Devilmentioning

confidence: 97%

A Histological and Immunohistochemical Analysis of Lymphoid Tissues of the Tasmanian Devil

Kreiss

Obendorf

Hemsley

et al. 2009

The Anatomical Record

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Tasmanian devil lymphoid tissues (thymus, spleen, and lymph node) from seven animals, including pouch young, juvenile, and adult devils, were investigated using histological and immunohistochemical techniques. Antibodies against the conserved intracytoplasmic portion of CD3 and CD79b (T-and B-cell markers, respectively) and MHC II were used to label immune cells. The thymus from the juvenile devils and the pouch young had CD3þ cells that were primarily located in the medulla of the organ. The spleen consisted of red and white pulp areas with characteristic lymphoid follicles with CD79bþ and MHC IIþ cells and nonfollicular T-cell-dominated periarteriolar lymphoid sheaths. Peripheral lymph nodes presented three distinct regions, outer cortex and medulla (both with primarily CD79bþ and MHC IIþ cells) and paracortex (mainly CD3þ cells). Tissue architecture and distribution of the immune cells were similar to that seen in eutherian mammals and other marsupials, indicating that the Tasmanian devil has all the structural elements necessary for effective adaptive immunity. Anat Rec, 292:611-620, 2009. V V C 2009 Wiley-Liss, Inc.

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A comparative study of lymph node mast cell populations in five marsupial species

Cited by 16 publications

References 32 publications

Ultrastructural Changes in the Cervical Epithelium during the Estrous Cycle of the Marsupial <i>Monodelphis domestica</i> (Grey Short-Tailed Opossum)

Ultrastructural Changes in the Cervical Epithelium during the Estrous Cycle of the Marsupial <i>Monodelphis domestica</i> (Grey Short-Tailed Opossum)

Ultrastructural Changes in the Uterine Luminal and Glandular Epithelium during the Oestrous Cycle of the Marsupial <i>Monodelphis domestica</i> (Grey Short-Tailed Opossum)

A Histological and Immunohistochemical Analysis of Lymphoid Tissues of the Tasmanian Devil

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