A Comparative Analyses of the Complete Mitochondrial Genomes of Fungal Endosymbionts in Sogatella furcifera, White-Backed Planthoppers

Abstract

Sogatella furcifera Horvath, commonly known as the white-backed planthoppers (WBPH), is an important pest in East Asian rice fields. Fungal endosymbiosis is widespread among planthoppers in the infraorder Fulgoromorpha and suborder Auchenorrhyncha. We successfully obtained complete mitogenome of five WBPH fungal endosymbionts, belonging to the Ophiocordycipitaceae family, from next-generation sequencing (NGS) reads obtained from S. furcifera samples. These five mitogenomes range in length from 55,390 bp to 55,406 bp, which is shorter than the mitogenome of the fungal endosymbiont found in Ricania speculum, black planthoppers. Twenty-eight protein-coding genes (PCGs), 12 tRNAs, and 2 rRNAs were found in the mitogenomes. Two single-nucleotide polymorphisms, two insertions, and three deletions were identified among the five mitogenomes, which were fewer in number than those of four species of Ophiocordycipitaceae, Ophiocordyceps sinensis, Hirsutella thompsonii, Hirsutella rhossiliensis, and Tolypocladium inflatum. Noticeably short lengths (up to 18 bp) of simple sequence repeats were identified in the five WBPH fungal endosymbiont mitogenomes. Phylogenetic analysis based on conserved PCGs across 25 Ophiocordycipitaceae mitogenomes revealed that the five mitogenomes were clustered with that of R. speculum, forming an independent clade. In addition to providing the full mitogenome sequences, obtaining complete mitogenomes of WBPH endosymbionts can provide insights into their phylogenetic positions without needing to isolate the mtDNA from the host. This advantage is of value to future studies involving fungal endosymbiont mitogenomes.

The complete chloroplast genome of <I>Erigeron canadensis</I> isolated in Korea (Asteraceae): Insight into the genetic diversity of the invasive species

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Comprehensive analysis of the complete mitochondrial genome of Melanoplus differentialis (Acrididae: Melanoplinae) captured in Korea

“…Simple sequence repeats (SSRs) were identified on the mitochondrial genome sequence with the SSR database pipeline utilized in previous studies (Choi et al 2021; Kim et al 2019; Kim et al 2021e; Park et al 2020c; Park et al 2022a). We conducted this analysis according to usual definition of an SSR on the circular genome (mitochondria and chloroplast): monoSSR (1 bp), hexaSSR (6 bp), and decaSSR (10 bp) (Lee et al 2020b; Park et al, 2021e).…”

The complete chloroplast genome of Campsis grandiflora (Bignoniaceae)

“…Simple sequence repeats (SSRs) were identified on the chloroplast genome sequence using the pipeline of the SSR database (SSRDB; http://ssrdb.infoboss.co.kr/) which has been utilized in several organelle genomic studies (Lee et al, 2020;Choi et al, 2021;Park et al, 2021dPark et al, , 2022. The SSR is conventionally recognized as the nucleotide array composed of repeats with one or up to six base pair units.…”