A combinatorial variation in surface chemistry and pore size of three-dimensional porous poly(ε-caprolactone) scaffolds modulates the behaviors of mesenchymal stem cells

Zhao, Yingdi; Tan, Ke Tian; Zhou, Yan; Ye, Zhaoyang; Tan, Wen‐Song

doi:10.1016/j.msec.2015.10.017

Cited by 61 publications

(52 citation statements)

References 56 publications

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“…In total, 277 gene expressions were either detected uniquely for a particular sample or shown to be differentially up-regulated or down-regulated by different culture conditions. Such a differential response of MSCs, in terms of proliferative and differentiation potential, towards surface topography or surface chemistry have been reported in the literature [25,49,50,51]. Taking the advantages of transcriptome sequencing, DEGs were sorted to eight sets to further understand the topography (Figure 5b) and chemistry impact (Figure 5c) on altering hbm-MSCs gene expression patterns.…”

Section: Resultsmentioning

confidence: 62%

Differential and Interactive Effects of Substrate Topography and Chemistry on Human Mesenchymal Stem Cell Gene Expression

Zhang

Kasoju

et al. 2018

IJMS

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Variations in substrate chemistry and the micro-structure were shown to have a significant effect on the biology of human mesenchymal stromal cells (hMSCs). This occurs when differences in the surface properties indirectly modulate pathways within numerous signaling networks that control cell fate. To understand how the surface features affect hMSC gene expression, we performed RNA-sequencing analysis of bone marrow-derived hMSCs cultured on tissue culture-treated polystyrene (TCP) and poly(l-lactide) (PLLA) based substrates of differing topography (Fl: flat and Fs: fibrous) and chemistry (Pr: pristine and Am: aminated). Whilst 80% of gene expression remained similar for cells cultured on test substrates, the analysis of differentially expressed genes (DEGs) revealed that surface topography significantly altered gene expression more than surface chemistry. The Fl and Fs topologies introduced opposite directional alternations in gene expression when compared to TCP control. In addition, the effect of chemical treatment interacted with that of topography in a synergistic manner with the Pr samples promoting more DEGs than Am samples in all gene ontology function groups. These findings not only highlight the significance of the culture surface on regulating the overall gene expression profile but also provide novel insights into cell-material interactions that could help further design the next-generation biomaterials to facilitate hMSC applications. At the same time, further studies are required to investigate whether or not the observations noted correlate with subsequent protein expression and functionality of cells.

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Section: Resultsmentioning

confidence: 62%

Differential and Interactive Effects of Substrate Topography and Chemistry on Human Mesenchymal Stem Cell Gene Expression

Zhang

Kasoju

et al. 2018

IJMS

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“…Facilitating the differentiation of MSCs down adipogenic, chondrogenic, or osteogenic pathways using a mimetic scaffold has long been desirable. It is well established that the chemistry and morphology of scaffolds influences the propensity for cells to undergo differentiation . Thus, investigating these variables and presenting direct comparisons of different polymers and morphologies is highly instructive for those wishing to design effective scaffolds.…”

Section: Discussionmentioning

confidence: 99%

“…It is well established that the chemistry and morphology of scaffolds influences the propensity for cells to undergo differentiation. 13,[22][23][24] Thus, investigating these variables and presenting direct comparisons of different polymers and morphologies is highly instructive for those wishing to design effective scaffolds. This work assessed how polymer chemistry and the fiber orientation of electrospun scaffolds affect adipogenic, chondrogenic, and osteogenic differentiation of MSCs.…”

Section: Discussionmentioning

confidence: 99%

A comparative evaluation of the effect of polymer chemistry and fiber orientation on mesenchymal stem cell differentiation

Rowland

Aquilina

Klein

et al. 2016

J Biomedical Materials Res

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Bioengineered tissue scaffolds in combination with cells hold great promise for tissue regeneration. The aim of this study was to determine how the chemistry and fiber orientation of engineered scaffolds affect the differentiation of mesenchymal stem cells (MSCs). Adipogenic, chondrogenic, and osteogenic differentiation on aligned and randomly orientated electrospun scaffolds of Poly (lactic‐co‐glycolic) acid (PLGA) and Polydioxanone (PDO) were compared. MSCs were seeded onto scaffolds and cultured for 14 days under adipogenic‐, chondrogenic‐, or osteogenic‐inducing conditions. Cell viability was assessed by alamarBlue metabolic activity assays and gene expression was determined by qRT‐PCR. Cell‐scaffold interactions were visualized using fluorescence and scanning electron microscopy. Cells grew in response to scaffold fiber orientation and cell viability, cell coverage, and gene expression analysis showed that PDO supports greater multilineage differentiation of MSCs. An aligned PDO scaffold supports highest adipogenic and osteogenic differentiation whereas fiber orientation did not have a consistent effect on chondrogenesis. Electrospun scaffolds, selected on the basis of fiber chemistry and alignment parameters could provide great therapeutic potential for restoration of fat, cartilage, and bone tissue. This study supports the continued investigation of an electrospun PDO scaffold for tissue repair and regeneration and highlights the potential of optimizing fiber orientation for improved utility. © 2016 The Authors Journal of Biomedical Materials Research Part A Published by Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2843–2853, 2016.

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“…The in vitro analysis proved the superior ability of PEEK‐SP to induce bone cell proliferation and differentiation. The particulate leaching method was also adopted by Zhao et al () who fabricated 3D porous PCL scaffolds with different macropore size to evaluate the hMSCs response to the macrotopography. Porous scaffolds were produced using paraffin microspheres (100–200, 200–300, and 300–450 μm) as porogen.…”

Section: Macroporesmentioning

confidence: 99%

Reviewing recently developed technologies to direct cell activity through the control of pore size: From the macro‐ to the nanoscale

2019

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Scaffold pore size plays a fundamental role in the regeneration of new tissue since it has been shown to direct cell activity in situ. It is well known that cellular response changes in relation with pores diameter. Consequently, researchers developed efficient approaches to realize scaffolds with controllable macro‐, micro‐, and nanoporous architecture. In this context, new strategies aiming at the manufacturing of scaffolds with multiscale pore networks have emerged, in the attempt to mimic the complex hierarchical structures found in living systems. In this review, we aim at providing an overview of the fabrication methods currently adopted to realize scaffolds with controlled, multisized pores highlighting their specific influence on cellular activity.

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A combinatorial variation in surface chemistry and pore size of three-dimensional porous poly(ε-caprolactone) scaffolds modulates the behaviors of mesenchymal stem cells

Cited by 61 publications

References 56 publications

Differential and Interactive Effects of Substrate Topography and Chemistry on Human Mesenchymal Stem Cell Gene Expression

Differential and Interactive Effects of Substrate Topography and Chemistry on Human Mesenchymal Stem Cell Gene Expression

A comparative evaluation of the effect of polymer chemistry and fiber orientation on mesenchymal stem cell differentiation

Reviewing recently developed technologies to direct cell activity through the control of pore size: From the macro‐ to the nanoscale

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