A combination of the cytokinesis-block micronucleus cytome assay and centromeric identification for evaluation of the genotoxicity of dicamba

González, Norma Viviana; Nikoloff, Noelia; Soloneski, Sonia; Larramendy, Marcelo L.

doi:10.1016/j.toxlet.2011.09.013

Cited by 21 publications

(24 citation statements)

References 41 publications

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“…As similar to the our results, the cytotoxic/cytostatic effects of some different synthetic auxins were reported in the previous studies performed on various test systems (Di Paolo et al 2001;Tuschl and Schwab 2003;Mičić et al 2004;Gonzàlez et al 2006Gonzàlez et al , 2007Gonzàlez et al , 2011.…”

Section: Discussionsupporting

confidence: 93%

“…Similar results were also reported by Zeljezic and Garaj-Vrhovac (2004), who observed that deberhan A, a commercial formulation of 2,4-D, caused an increase in chromatid and chromosome breaks, number of MNi and number of NBUDs in human PBLs. Gonzàlez et al (2011) found that dicamba and one of its commercial formulation banvel induced a significant increase in MN and NBUD formation at a concentration range of 50-500 lg/mL in Chinese hamster ovary cells. The damaging activity of dicamba in different mammalian cell targets was also previously observed by the same research group (Gonzàlez et al 2006(Gonzàlez et al , 2007(Gonzàlez et al , 2009).…”

Section: Discussionmentioning

confidence: 97%

“…NBUDs may be formed by exclusion of amplified DNA (Lindberg et al 2007), from remnants of broken anaphase bridges (Gisselsson et al 2000(Gisselsson et al , 2001 or by retraction of micronuclei (Lindberg et al 2007). Although there are no published data relevant to MN and NBUD formations induced by NAAm, it can be seen from the above mentioned literature that some synthetic auxins caused an increase in MN and NBUD frequency in vitro in human PBLs (Zeljezic and Garaj-Vrhovac 2004) and in Chinese hamster ovary cells (Gonzàlez et al 2011). Mladinic et al (2009) suggested that the development of NBUDs is not possible in the short-term cultivation period.…”

Section: Discussionmentioning

confidence: 99%

“…Unfortunately, to our best knowledge, there is no study available on the genotoxic and/ or cytotoxic effects of NAAm on humans in the literature. However, some synthetic auxins, such as 2,4-dichlorophenoxyacetic acid (2,4-D) (Holland et al 2002;Cenkci et al 2010), dicamba (Gonzàlez et al 2007(Gonzàlez et al , 2009Gonzàlez et al 2011), and synthetic IAA (Salopek-Sondi et al 2010) have been found genotoxic, mutagenic and cytotoxic in various test systems. Thus, taking into account these facts, it has become important to evaluate the possible genotoxic potential of NAAm on humans.…”

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confidence: 99%

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In vitro genotoxicity assessment of the synthetic plant growth regulator, 1-naphthaleneacetamide

Kocaman

Güven

2015

Cytotechnology

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1-Naphthaleneacetamide (NAAm) is a synthetic plant growth regulator in the auxin family that is widely used in agriculture to promote the growth of numerous fruits, for root cuttings and as a fruit thinning agent. The potential genotoxic effects of NAAm were investigated in vitro by the chromosome aberrations (CAs), and cytokinesis-block micronucleus assays in human peripheral blood lymphocytes (PBLs) for the first time. The human PBLs were treated with 20, 40, 80, and 160 lg/mL of NAAm for 24 and 48 h. The results of this study showed that NAAm significantly induced the formation of structural CA and MN for all concentrations (20, 40, 80 and 160 lg/mL) and treatment periods (24 and 48 h) when compared with the negative and the solvent control. In addition, the higher concentrations of NAAm (80 and 160 lg/mL) caused a statistically significant increase in nuclear bud (NBUD) formation for both 24 and 48 h treatment times. With regard to the cell cycle kinetics, at all the tested concentrations, NAAm caused a statistically significant reduction in the mitotic index (MI) only for 48 h treatment period and also in the nuclear division index (NDI) for both 24 and 48 h treatment periods as compared to the control groups. The reductions in the MI and NDI occured in a concentration-dependent manner for both treatment times. In conclusion, the present results indicate that in the tested experimental conditions, NAAm was genotoxic and cytotoxic on human PBLs in vitro.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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In vitro genotoxicity assessment of the synthetic plant growth regulator, 1-naphthaleneacetamide

Kocaman

Güven

2015

Cytotechnology

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“…The use of in vitro cell cultures for genotoxic and cytotoxic evaluation is rather economic and they are highly sensitive methods for the early detection of chemical exposure and toxicity (DiPaolo et al 1981). Among them, one of the most used system for clastogenic and/or aneugenic screening is the micronucleus assay in human peripheral blood lymphocytes (Ali et al 2011;González et al 2011;Nikoloff et al 2012b;Soloneski et al 2008;Vera-Candioti et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Micronucleus assay in human lymphocytes after exposure to alloxydim sodium herbicide in vitro

et al. 2014

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This study evaluates the cytotoxic and genotoxic potential of alloxydim sodium using micronucleus (MN) assay, in human peripheral lymphocytes. MN assay was used to investigate the genotoxic effects of alloxydim sodium in human peripheral lymphocytes treated with 250, 500, 750, 1,000 lg/ml concentrations of alloxydim sodium for 24 and 48 h. Solvent, negative and positive controls were also used in the experiments in parallel. The obtained results were evaluated in statistical analyses by using Dunnett-t test (two sided) and p \ 0.05 was accepted as significant. Alloxydim sodium significantly increased the MN formation compared with the negative control, at both 750 and 1,000 lg/ml concentrations and treatment periods. We also evaluated the nuclear division index (NDI) for cytotoxicity of this pesticide in the experiment, and finally observed a significant decrease of the NDI values at all concentrations of alloxydim sodium and at both treatment periods.

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Comparative evaluationin vitroof the herbicide flurochloridone by cytokinesis-block micronucleus cytome and comet assays

2012

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The in-vitro effects of flurochloridone and its formulations Twin Pack Gold® (25% a.i.) and Rainbow® (25% a.i.) were evaluated in Chinese Hamster Ovary K1 (CHO-K1) cells. The cytokinesis-block micronucleus cytome (CBMN-cyt) and single-cell gel electrophoresis (SCGE) assays were used. The activities were tested within the range of final concentrations of 0.25-15 μg flurochloridone/mL. The results demonstrated that both the flurochloridone and Rainbow® were not able to induce micronuclei (MN). On the other hand, Twin Pack Gold® only increased the frequency of MN at 5 μg/mL. Furthermore, 10 and 15 μg/mL of both formulations resulted in a cellular cytotoxicity demonstrated by alterations in the nuclear division index and cellular death. SCGE assay appeared to be a more sensitive bioassay for detecting primary DNA strand breaks at lower concentrations of flurochloridone than MN did. A marked increase in the genetic damage index was observed when 5 and 15 μg/mL of both flurochloridone and Rainbow® but only when 15 μg/mL of Twin Pack Gold® were used. This is the first report demonstrating that flurochloridone and its two commercial formulations are able to induce single-strand DNA breaks in vitro on mammalian cells.

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A combination of the cytokinesis-block micronucleus cytome assay and centromeric identification for evaluation of the genotoxicity of dicamba

Cited by 21 publications

References 41 publications

In vitro genotoxicity assessment of the synthetic plant growth regulator, 1-naphthaleneacetamide

In vitro genotoxicity assessment of the synthetic plant growth regulator, 1-naphthaleneacetamide

Micronucleus assay in human lymphocytes after exposure to alloxydim sodium herbicide in vitro

Comparative evaluationin vitroof the herbicide flurochloridone by cytokinesis-block micronucleus cytome and comet assays

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