A colorimetric assay for sensitive detection of hydrogen peroxide and glucose in microfluidic paper-based analytical devices integrated with starch-iodide-gelatin system

Liu, M.; Lian, Xin; Liu, Hui; Guo, Zi-Zhen; Huang, Huihong; Lei, Yun; Peng, Hua-Ping; Chen, Wei; Lin, Xinhua; Liu, Ailin; Xia, Xing‐Hua

doi:10.1016/j.talanta.2019.03.089

Cited by 65 publications

(23 citation statements)

References 35 publications

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“…The sensor gave a linear response for glucose concentration range between 0.1 and 1 mM (R 2 = 0.9819) in artificial saliva with a calculated LOD of 29.65 µM, which is low as compared to some papers found in literature, especially those using KI as indicator (Fig. 4B I ) [8], [26], [33]- [36]. The selectivity of the sensor was also tested against some interfering species and the results showed that the color intensity in the presence of these species was comparable to that of saliva with no glucose (Fig.…”

Section: Resultsmentioning

confidence: 69%

“…4A II , the relative color intensity of all samples became almost steady after 30 s, which suggests that the analysis can be completed in less than 1 min (Video S1, ESI). The analysis with filter-paper based µPADs is usually completed within 10 to 20 min [31]- [33]. In light of this result, glucose measurement time can be significantly shortened using the proposed strategy as compared to the duration of the analysis with µPADs made of filter papers.…”

Section: Resultsmentioning

confidence: 99%

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A Portable Smartphone-Based Platform with an Offline Image Processing Tool for Rapid Paper-Based Colorimetric Detection of Glucose in Artificial Saliva

Golcez¹,

Kılıç²,

Şen³

2020

Preprint

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Paper-based sensors have great potential for use in a variety of areas, from environmental monitoring to clinical and pointof-care testings. Here, a microfluidic paper-based analytical device (µPAD) was integrated with a smartphone app capable of offline (without internet access) image processing and analysis for rapid colorimetric detection of glucose. A self-inking stamp was used to form hydrophobic channels on a piece of paper-towel due to its superior water absorption efficiency. As demonstrated, the developed sensor was employed for colorimetric detection of glucose in artificial saliva in the linear scope of 0-1 mM with a calculated detection limit of 29.65 µM. In addition, experimental results show that quantitative analysis of glucose with the proposed smartphone platform could be completed in less than one minute. The app developed for the smartphone platform is capable of extracting the color changing area with an embedded image processing tool which could address the problem of color uniformity in detection zones of µPAD. The total cost of a µPAD is less than $0.2. By integrating µPAD with a smartphone and user-friendly app together, the proposed smartphone-based platform could be used for the quantitative analysis of glucose with advantages such as portability, simple operation, rapid response, ultra-low cost, field-deployable, selectivity and sensitivity. The results show that the integrated platform has great potential to be used for non-invasive measurement of glucose in body fluids like a tear, sweat and saliva.

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Section: Resultsmentioning

confidence: 69%

Section: Resultsmentioning

confidence: 99%

A Portable Smartphone-Based Platform with an Offline Image Processing Tool for Rapid Paper-Based Colorimetric Detection of Glucose in Artificial Saliva

Golcez¹,

Kılıç²,

Şen³

2020

Preprint

View full text Add to dashboard Cite

show abstract

“…6a and b reveals the two‐fold increase in the colour intensity of 100 and 150 mg/dL, respectively. Recently, growing body of literature attempted to enhance the sensitivity of colorimetric detection for glucose with the detection limit of 1 mg/ dL . Despite its invasive nature, “blood” is still regarded as holy grail for disease diagnosis.…”

Section: Resultsmentioning

confidence: 99%

Electro‐kinetically driven route for highly sensitive blood pathology on a paper‐based device

et al. 2020

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Electro-kinetically driven route for highly sensitive blood pathology on a paper-based deviceEnhancing the sensitivity of colorimetric detection in paper-devices is a quintessential step in achieving frugal diagnosis. Here, we demonstrate an effective way of improving the detection sensitivity of paper-based devices, as mediated by electro-kinetic mechanisms. By directly employing blood plasma, we investigate the electro-kinetic clustering of glucose, a neutral molecule in paper devices. Under the influence of uniform electric field, dispersed glucose gets accumulated in the paper strips. Due to the combination of EOF and electrophoretic migration, we achieve twofold increase in the colour intensity for both normal and diabetic samples. This approach is robust and possesses better sensitivity than conventional colorimetric assays and can be easily extended to other body fluid based diagnosis. These results may turn out to be of profound importance in improving the quality of pathological diagnosis in low-cost paper-based point-of-care devices deployed in resourcelimited settings.

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“…The vast majority of current biochemical assays relies on colorimetric detections, particularly enzyme-linked immunosorbent assay (ELISA) produces a colored solution in the presence of the analyte [4]. Based on the principle that the solution absorbance has a direct correlation with the analyte concentration [5], many standard ELISA protocols have been established and widely utilized in biomedical analysis for decades [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

A WiFi scanner in conjunction with disposable multiplex paper assay for the quantitation of disease markers in blood plasma

et al. 2021

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Herein we report a quantitative, multiplex assay for disease markers in plasma based on an integrated setup of a portable scanner and a disposable paper-based analytical device (PAD). The quantitative analysis relies on the digital colorimetric reading of the three-layer PAD with 30 assay sites for performing respective chromogenic reactions for plasma uric acid, glucose, and triglyceride, which are considered as important risk factors for cardiovascular diseases. A portable scanner with WiFi transmission capability was used to produce high-quality color images of the PADs and wirelessly transfer them to a smartphone or other mobile devices for data processing. The concentrations of biomarkers in both standard solutions and plasma samples can be directly obtained using a custom-designed smartphone app that is also capable of constructing calibration curves. The detection limits of uric acid, glucose, and triglyceride were determined to be 0.50 mg/dL, 0.84 mmol/L, and 14 mg/dL, respectively, which are below the normal limits and adequate for clinical validation. Owing to the distinct advantages-simple, portable, and costeffective-this mobile assay protocol can be used for point-of-care (POC) settings or resource-limited situations, and potentially for the diagnosis and prevention of infectious diseases.

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A colorimetric assay for sensitive detection of hydrogen peroxide and glucose in microfluidic paper-based analytical devices integrated with starch-iodide-gelatin system

Cited by 65 publications

References 35 publications

A Portable Smartphone-Based Platform with an Offline Image Processing Tool for Rapid Paper-Based Colorimetric Detection of Glucose in Artificial Saliva

A Portable Smartphone-Based Platform with an Offline Image Processing Tool for Rapid Paper-Based Colorimetric Detection of Glucose in Artificial Saliva

Electro‐kinetically driven route for highly sensitive blood pathology on a paper‐based device

A WiFi scanner in conjunction with disposable multiplex paper assay for the quantitation of disease markers in blood plasma

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