A chloramphenicol-streptomycin-resistance plasmid from a clinical strain of Staphylococcus sciuri and its structural relationships to other staphylococcal resistance plasmids

Schwarz, St.

doi:10.1016/0378-1097(91)90281-e

Cited by 12 publications

(17 citation statements)

References 18 publications

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“…Small plasmids of approximately 4.5 kbp which closely resemble pS194 in their restriction maps have been detected in porcine isolates of S. hyicus [71], but also in S. warneri isolates of feline and avian origin as well as in S. lentus isolates from pigeons (Schwarz and Werckenthin, unpublished data). A pS194-like str gene was also detected on naturally occurring plasmids from porcine S. hyicus and bovine S. sciuri which confer chloramphenicol and streptomycin resistance [76,77].…”

Section: Resistance To Aminoglycosides and Aminocyclitolsmentioning

confidence: 99%

“…Cm resistance plasmids of the pC221-family vary in size from 2.9-5.1 kbp and occasionally carry a streptomycin (Sm) resistance gene. Two naturally occurring Cm/Sm resistance plasmids have been detected in animal staphylococci, one in a bovine S. sciuri [76] and the other in a porcine S. hyicus [77]. Both plasmids are believed to have developed by sitespecific recombination processes between a pC221-like Cm resistance plasmid and a pS194-analogous Sm resistance plasmid [76,77].…”

Section: Resistance To Chloramphenicol and Florfenicolmentioning

confidence: 99%

“…Two naturally occurring Cm/Sm resistance plasmids have been detected in animal staphylococci, one in a bovine S. sciuri [76] and the other in a porcine S. hyicus [77]. Both plasmids are believed to have developed by sitespecific recombination processes between a pC221-like Cm resistance plasmid and a pS194-analogous Sm resistance plasmid [76,77]. Comparative data on the catA gene and its gene product are only available for the determinants located on plasmids pC221 and pSCS6 of the pC221-family and plasmids pSCS5 and pSCS7 of the pC223 family [10,12,74,75].…”

Section: Resistance To Chloramphenicol and Florfenicolmentioning

confidence: 99%

“…On reaching the new host cell, this linkage is usually resolved, however in some cases, stable plasmid cointegrates are also observed. The rarely detected naturally occurring Cm/Sm resistance plasmids [24,76,77] may represent examples for such cointegrates. Efficient transduction also requires specific receptors for the attachment of the transducing phages on the surface of the new host cell.…”

Section: Horizontal Gene Transfermentioning

confidence: 99%

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Antimicrobial resistance in staphylococci from animals with particular reference to bovine Staphylococcus aureus, porcine Staphylococcus hyicus, and canine Staphylococcus intermedius

et al. 2001

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-Besides their role as commensals on the skin and mucosal surfaces, staphylococci may be involved in a wide variety of diseases in animals. Staphylococcal infections in animals are mainly treated with antimicrobial agents and as a consequence, staphylococci from animal sources have developed and/or acquired resistance to the respective antimicrobial agents. Resistance statistics obtained from national monitoring programmes on staphylococci from cattle and pigs, but also from surveillance studies on staphylococci involved in diseases in dogs are reported and reviewed with regard to their comparability. This review mainly focusses on the genetic basis of antimicrobial resistance in staphylococci of animal origin. Particular attention is paid to resistance to those antimicrobial agents which are most frequently used in veterinary medicine, but also to antimicrobial agents, such as chloramphenicol and mupirocin, which are used in specific cases for the control of staphylococcal infections in pets and companion animals. In addition, plasmids and transposons associated with the respective resistance properties and their ways of spreading between members of the same or different staphylococcal species, but also between staphylococci and other gram-positive bacteria, are described.antimicrobial resistance / Staphylococcus / animal origin / epidemiology / horizontal gene transfer Vet. Res. 32 (2001) 341

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Section: Resistance To Aminoglycosides and Aminocyclitolsmentioning

confidence: 99%

Section: Resistance To Chloramphenicol and Florfenicolmentioning

confidence: 99%

Section: Resistance To Chloramphenicol and Florfenicolmentioning

confidence: 99%

Section: Horizontal Gene Transfermentioning

confidence: 99%

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Antimicrobial resistance in staphylococci from animals with particular reference to bovine Staphylococcus aureus, porcine Staphylococcus hyicus, and canine Staphylococcus intermedius

et al. 2001

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“…Staphylococcus sciuri, a common inhabitant of the skin of rodents and other mammals, has been reported to carry a number of resistance plasmids, such as the tetracycline resistance plasmid pSTS9 (12), the chloramphenicol resistance plasmid pSCS13 (10), and also the chloramphenicol-streptomycin resistance plasmid pSCS12 (11), which differ in size and/or structure from the resistance plasmids commonly found in staphylococci. More recently, the first and, to date, only known staphylococcal chloramphenicol-florfenicol resistance plasmid was isolated from a bovine S. sciuri isolate (13).…”

mentioning

confidence: 99%

Staphylococcus sciuri Gene erm (33), Encoding Inducible Resistance to Macrolides, Lincosamides, and Streptogramin B Antibiotics, Is a Product of Recombination between erm (C) and erm (A)

Schwarz

Kehrenberg

Ojo

2002

Antimicrob Agents Chemother

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A gene which mediates inducible resistance to macrolides, lincosamides, and streptogramin B antibiotics, designated erm(33), was detected on the Staphylococcus sciuri plasmid pSCFS1. Analysis of the erm(33) reading frame suggested that this gene was the product of a recombination between an erm(C) gene and an erm(A) gene. Such a recombination event is a novel observation for erm genes.Staphylococcus sciuri, a common inhabitant of the skin of rodents and other mammals, has been reported to carry a number of resistance plasmids, such as the tetracycline resistance plasmid pSTS9 (12), the chloramphenicol resistance plasmid pSCS13 (10), and also the chloramphenicol-streptomycin resistance plasmid pSCS12 (11), which differ in size and/or structure from the resistance plasmids commonly found in staphylococci. More recently, the first and, to date, only known staphylococcal chloramphenicol-florfenicol resistance plasmid was isolated from a bovine S. sciuri isolate (13). Analysis of this plasmid, designated pSCFS1, showed that it also mediated inducible resistance to macrolides, lincosamides, and streptogramin B antibiotics (MLS B antibiotics). Based on the results of PCR analysis, the MLS B resistance gene of plasmid pSCFS1 was considered to be an erm gene of class C (13). Since erm(C) genes are commonly located on small 2.3-to 4.3-kb plasmids (1-3, 16) and have very rarely been detected on larger plasmids, we decided to clone and sequence the erm gene and its adjacent regions of the ca. 17-kb plasmid pSCFS1. This approach should provide information on how the erm(C) gene has become part of plasmid pSCFS1.To localize the erm gene in plasmid pSCFS1, hybridization studies were conducted with a gene probe that consisted of the 378-bp SacI-BclI fragment of plasmid pSES5 (3). This gene probe comprised the entire erm(C) translational attenuator and the first 219 bp of the erm(C) gene. The smallest hybridizing fragment was an EcoRI-PstI fragment of ca. 2.2 kb. This fragment was cloned into pBluescript II SK(ϩ), and the recombinant plasmid was transformed into Escherichia coli JM107. Sequence analysis on both strands was performed by primer walking, starting with the M13 universal and reverse primers.The sequence of this EcoRI-PstI fragment consisted of 2,196 bp. At the EcoRI end, the first 602 bp represented the 5Ј end of a reading frame whose product showed similarity to plasmid-borne recombination-mobilization proteins of gram-positive bacteria (Fig. 1). The amino terminal 200 amino acids (aa) of this reading frame were most closely related to the corresponding parts of a recombination-mobilization protein of Listeria monocytogenes (U40997) with 82% amino acid identity, the recombination protein of the Bacillus plasmid pTB913 (X15670; 81% amino acid identity), the mobilization protein of Geobacillus stearothermophilus (M63891; 81% amino acid identity), and the recombination-mobilization protein of Staphylococcus cohnii (AF015628; 79% amino acid identity). Sequence homology to the expected erm(C) region started about 340 bp ...

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Molecular basis of bacterial resistance to chloramphenicol and florfenicol

et al. 2004

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Chloramphenicol (Cm) and its fluorinated derivative florfenicol (Ff) represent highly potent inhibitors of bacterial protein biosynthesis. As a consequence of the use of Cm in human and veterinary medicine, bacterial pathogens of various species and genera have developed and/or acquired Cm resistance. Ff is solely used in veterinary medicine and has been introduced into clinical use in the mid-1990s. Of the Cm resistance genes known to date, only a small number also mediates resistance to Ff. In this review, we present an overview of the different mechanisms responsible for resistance to Cm and Ff with particular focus on the two different types of chloramphenicol acetyltransferases (CATs), specific exporters and multidrug transporters. Phylogenetic trees of the different CAT proteins and exporter proteins were constructed on the basis of a multisequence alignment. Moreover, information is provided on the mobile genetic elements carrying Cm or Cm/Ff resistance genes to provide a basis for the understanding of the distribution and the spread of Cm resistance--even in the absence of a selective pressure imposed by the use of Cm or Ff.

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A chloramphenicol-streptomycin-resistance plasmid from a clinical strain of Staphylococcus sciuri and its structural relationships to other staphylococcal resistance plasmids

Cited by 12 publications

References 18 publications

Antimicrobial resistance in staphylococci from animals with particular reference to bovine Staphylococcus aureus, porcine Staphylococcus hyicus, and canine Staphylococcus intermedius

Antimicrobial resistance in staphylococci from animals with particular reference to bovine Staphylococcus aureus, porcine Staphylococcus hyicus, and canine Staphylococcus intermedius

Staphylococcus sciuri Gene erm (33), Encoding Inducible Resistance to Macrolides, Lincosamides, and Streptogramin B Antibiotics, Is a Product of Recombination between erm (C) and erm (A)

Molecular basis of bacterial resistance to chloramphenicol and florfenicol

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