A Chinese herbal decoction, Danggui Buxue Tang, activates extracellular signal‐regulated kinase in cultured T‐lymphocytes

Abstract

Danggui Buxue Tang (DBT) is prepared from Radix Astragali and Radix Angelicae Sinensis. This Chinese herbal decoction has been shown to stimulate the proliferation of T-lymphocytes; however, the action mechanism of this stimulation has not been revealed. In cultured T-lymphocytes, application of DBT markedly induced the cell proliferation, the release of interleukin-2, -6 and -10, as well as the phosphorylation of extracellular signal-regulated kinases (ERK). The pre-treatment of ERK inhibitor blocked the DBT-induced immune responses. In addition, the polysaccharide-enriched fraction of DBT showed marked responses on the cultured T-lymphocytes suggesting the important role of DBT polysaccharide in triggering such immune responses.

Cordysinocan, a polysaccharide isolated from cultured Cordyceps, activates immune responses in cultured T-lymphocytes and macrophages: Signaling cascade and induction of cytokines

“…Human T-lymphocytes were isolated from freshly collected blood of health donors by Ficoll-Hypaque according to previous method (Gao et al, 2007). The purified T-lymphocytes were washed twice with RPMI-1640 medium and seeded at a density of 1 × 10 5 cells/well in a 96-well culture plate.…”

“…In the phosphorylation studies, cultured T-lymphocytes in serum-free medium were treated with cordysinocan at 20 g/ml. The detailed procedure was described previously (Gao et al, 2007). The phosphorylation of ERK1/2 was determined by Western blot assay.…”

Identification of metabolites of Danggui Buxue Tang in rat urine by liquid chromatography coupled with electrospray ionization time‐of‐flight mass spectrometry

“…3 Nowadays, DBT is used not only as an efficacious medicine, but also as a common food supplement. Previous studies have demonstrated that DBT has attractive pharmacological activities, including immunoregulation, 4 estrogenic properties, 5 anti-inflammation, 6 stimulation of the T-lymphocytes proliferation, 7 regulating blood lipid and foam cell formation, 8 as well as retarding the progress of diabetic nephropathy. 9 In recent years, metabolism studies of CHMs have attracted significant attention, as they shed additional light on drug efficacy, 10 and enable the screening of bioactive components, 11 the detection of potentially toxic metabolites, 12 the elucidation of herb-drug interactions, 13 and the identification of drug degradation pathways.…”

Effects of the Chinese Herbal FormulaShen Qi Yi Mu Tangon the Cellular Immunity of Postpartum Dairy Cows

“…The pre-treatment of ERK inhibitor blocked t h e DBT-induced immune responses. I n addition, t h e polysaccharide-enriched fraction of D B T showed marked responses on the cultured Tlymphocytes suggesting the important role of DBT polysaccharide in triggering such immune responses (Gao et al, 2007). Dang-Gui-Bu-Xai-T a n g (DGBXT), which includes R a d i x angelicae sinensis a n d R a d i x astragali membranaceus, markedly inhibited the growth of the EJHa-ras transformed LZEJ and LZEJ-C2 cells lines.…”