A Chemiluminescence Reaction Between Hydroxyl Radical and Rhodamine 6G and its Applications

Abstract

A novel chemiluminescence (CL) reaction between hydroxyl radical and ascorbic acid is described in this paper. Hydroxyl radical generated on line by the reaction between Fe 3þ solution and H 2 O 2 solution in HCl medium could oxidize rhodamine 6G to produce weak chemiluminescence. It was found that ascorbic acid could enhance the chemiluminescence and the excited rhodamine 6G was the emitter of the chemiluminescence reaction. The possible mechanism of the CL system was also discussed. Ascorbic acid can be determined in the range of 2.0 Â 10 26 28.0 Â 10 24 mg/ml with a detection limit of 1 Â 10 26 mg/ml (3s). A complete analysis could be done in 1 minute with the relative standard deviation of 3.1% for 5.0 Â 10 25 mg/ml (n ¼ 11). In order to study the chemiluminescence reaction further, the application to the determination of ascorbic acid in food using the chemiluminescence reaction combined with flow injection is investigated.

Determination of lansoprazole in pharmaceuticals using flow injection with rhodamine 6G–diperiodatoargentate(III) chemiluminescence detection

“…Therefore, according to the CRET (collision resonance energy transfer) principle, energy is transferred to the non‐oxidized R6G molecule, creating an excited R6G that emits light when excited at 560 nm. [ 50 ] The high quantum yield (0.95) shows that R6G has high fluorescence properties. [ 51 ]…”

Flow‐injection determination of manganese (II) using surfactant enhanced diperiodatonickelate (IV)‐rhodamine 6G chemiluminescence

“…Rh6‐G has been used as an enhancer in FI CL systems with Ag(III), Ce(IV) and KMnO 4 in acidic conditions for cefazolin, total phenolic compounds and tsumacide assays. Rh6‐G has been oxidized to an excited state by hydroxyl radicals generated in the presence of Fe(III) with H 2 O 2 for ascorbic acid detection and transferred energy to an non‐oxidized Rh6‐G molecule that generated strong CL emission monitored at 560 nm . Rh6‐G is a highly fluorescent compound with quantum yields of 0.95 …”

An Automated Method for Determination of Ascorbic Acid in Urine by Flow Injection Chemiluminescence Coupling With on-Line Removal of Interference

“…Flow injection chemiluminescence (FI-CL) offers the advantages of simplicity of apparatus, high sensitivity, high analysis speed and good reproducibility for determination of ascorbic acid in simple samples including vitamin C injections, vitamin C tablets and foods, [17][18][19][20][21][22][23][24] however, to the best of our knowledge, FI-CL have not been used for the determination of ascorbic acid in complex samples such as urine thus far due to the poor selectivity of chemiluminescent analysis and the complexity of urine samples. In this work, a simple and automated method was presented for determination of AA in urine samples by FI-CL coupling with a micro column of exchange resin.…”