A chemical method for estimating methanogenic biomass

The Historical Development of Cultivation Techniques for Methanogens and Other Strict Anaerobes and Their Application in Modern Microbiology

“…where µ is the specific growth rate (h −1 ), OD 1 and OD 2 are the optical density values determined at the respective times t 1 and t 2 (h). Optical density of methanogens is often measured at wavelength 578 nm [138][139][140] but it could be also measured at wavelength 600 or 660 [74,141]. The measured optical density has to be multiplied by a conversion factor, which is specific to every organism and must be experimentally determined, to obtain data for biomass or number of cells per volume (Equation ( 3)):…”

The Historical Development of Cultivation Techniques for Methanogens and Other Strict Anaerobes and Their Application in Modern Microbiology

“…where µ is the specific growth rate (h −1 ), OD 1 and OD 2 are the optical density values determined at the respective times t 1 and t 2 (h). Optical density of methanogens is often measured at wavelength 578 nm [138][139][140] but it could be also measured at wavelength 600 or 660 [74,141]. The measured optical density has to be multiplied by a conversion factor, which is specific to every organism and must be experimentally determined, to obtain data for biomass or number of cells per volume (Equation ( 3)):…”

Biomass Measurement of Methanogens in the Sediments of Tokyo Bay Using Archaeol Lipids

“…In contrast, high concentrations of 267 to 4160 ng/dry g or 51 to 430 ng/cm 3 were detected at the innermost area. Amounts of 303 ng/g (Smith and Floodgate, 1992) and 46 ng/g (Martz et al, 1983) of archaeol were reported at Menai Strait, N. Wales, and Florida State Marine Laboratory, respectively. The abundance of archaeol is thought to be controlled by the inputs originating from methanogenic growth and the degradation caused by other bacterial activities.…”

The Historical Development of Cultivation Techniques for Methanogens and Other Strict Anaerobes and Their Application in Modern Microbiology

“…where µ is the specific growth rate (h −1 ), OD 1 and OD 2 are the optical density values determined at the respective times t 1 and t 2 (h). Optical density of methanogens is often measured at wavelength 578 nm [138][139][140] but it could be also measured at wavelength 600 or 660 [74,141]. The measured optical density has to be multiplied by a conversion factor, which is specific to every organism and must be experimentally determined, to obtain data for biomass or number of cells per volume (Equation ( 3)):…”

Biomass Measurement of Methanogens in the Sediments of Tokyo Bay Using Archaeol Lipids

“…In contrast, high concentrations of 267 to 4160 ng/dry g or 51 to 430 ng/cm 3 were detected at the innermost area. Amounts of 303 ng/g (Smith and Floodgate, 1992) and 46 ng/g (Martz et al, 1983) of archaeol were reported at Menai Strait, N. Wales, and Florida State Marine Laboratory, respectively. The abundance of archaeol is thought to be controlled by the inputs originating from methanogenic growth and the degradation caused by other bacterial activities.…”

The Historical Development of Cultivation Techniques for Methanogens and Other Strict Anaerobes and Their Application in Modern Microbiology

“…where µ is the specific growth rate (h −1 ), OD 1 and OD 2 are the optical density values determined at the respective times t 1 and t 2 (h). Optical density of methanogens is often measured at wavelength 578 nm [138][139][140] but it could be also measured at wavelength 600 or 660 [74,141]. The measured optical density has to be multiplied by a conversion factor, which is specific to every organism and must be experimentally determined, to obtain data for biomass or number of cells per volume (Equation ( 3)):…”

Biomass Measurement of Methanogens in the Sediments of Tokyo Bay Using Archaeol Lipids

“…In contrast, high concentrations of 267 to 4160 ng/dry g or 51 to 430 ng/cm 3 were detected at the innermost area. Amounts of 303 ng/g (Smith and Floodgate, 1992) and 46 ng/g (Martz et al, 1983) of archaeol were reported at Menai Strait, N. Wales, and Florida State Marine Laboratory, respectively. The abundance of archaeol is thought to be controlled by the inputs originating from methanogenic growth and the degradation caused by other bacterial activities.…”