A Cell ELISA for the quantification of MUC1 mucin (CD227) expressed by cancer cells of epithelial and neuroectodermal origin

Falahat, Rana; Wiranowska, Marzenna; Gallant, Nathan D.; Toomey, Ryan; Hill, Robert; Alcantar, Norma

doi:10.1016/j.cellimm.2015.09.009

Cited by 23 publications

(9 citation statements)

References 36 publications

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“…MUC1 , as a master regulator of oncogenes, plays a vital role in cell proliferation, apoptosis resistance, and cell adhesion 29 . Recently, a study revealed significantly higher expression of the MUC1 protein in tumor cells than in normal cells through a specific cell ELISA technology, indicating that MUC1 may play an important role in carcinogenesis 30 . Another gene, PKLR , which was identified in our study, was also found to be a key regulator gene in carcinogenesis 31 …”

Section: Discussionsupporting

confidence: 51%

Predictive model for risk of gastric cancer using genetic variants from genome‐wide association studies and high‐evidence meta‐analysis

Qiu

et al. 2020

Cancer Medicine

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Section: Discussionsupporting

confidence: 51%

Predictive model for risk of gastric cancer using genetic variants from genome‐wide association studies and high‐evidence meta‐analysis

Qiu

et al. 2020

Cancer Medicine

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“…There are conventional immunoanalytical techniques and kits for accurate determination of MUC1. However, these are commonly based on enzyme linked immunosorbent assay (ELISA) methods that need expensive immune-chemicals, long response times and experienced people [68][69][70]. In this manner, molecular imprinted polymer technology can be a good alternative for the development of electrochemical affinity sensors for detection of biomarkers with high selectivity [71].…”

Section: Discussionmentioning

confidence: 99%

A New Nanomaterial Based Biosensor for MUC1 Biomarker Detection in Early Diagnosis, Tumor Progression and Treatment of Cancer

Ulucan‐Karnak

Akgöl

2021

Nanomanufacturing

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Early detection of cancer disease is vital to the successful treatment, follow-up and survival of patients, therefore sensitive and specific methods are still required. Mucin 1 (MUC1) is a clinically approved biomarker for determining the cancer that is a type I transmembrane protein with a dense glycosylated extracellular domain extending from the cell surface to 200–500 nm. In this study, nanopolymers were designed with a lectin affinity-based recognition system for MUC1 detection as a bioactive layer on electrochemical biosensor electrode surfaces. They were synthesized using a mini emulsion polymerization method and derivatized with triethoxy-3-(2-imidazolin-1-yl) propylsilane (IMEO) and functionalized with Concanavalin a Type IV (Con A) lectin. Advanced characterization studies of nanopolymers were performed. The operating conditions of the sensor system have been optimized. Biosensor validation studies were performed. Real sample blood serum was analyzed and this new method compared with a commercially available medical diagnostic kit (Enzyme-Linked ImmunoSorbent Assay-ELISA). The new generation nanopolymeric material has been shown to be an affordable, sensitive, reliable and rapid device with 0.1–100 U/mL linear range and 20 min response time.

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“…ELISA was performed as described previously ( 21 ). Briefly, 3×10 4 cells were seeded in a flat-bottomed 96-well microtiter plate and incubated for 24 h at 37°C in 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

EPCR promotes MGC803 human gastric cancer cell tumor angiogenesis in�vitro through activating ERK1/2 and AKT in a PAR1‑dependent manner

et al. 2018

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The endothelial cell protein C receptor (EPCR) serves a key role in activated protein C (APC)-mediated cytoprotective effects in endothelial cells, and is involved in the development of certain types of human cancer. To the best of our knowledge, the present study is the first to demonstrate that EPCR may exert effects on gastric cancer angiogenesis in vitro. To detect microvessel density (MVD), the microvascular endothelial cells were stained for cluster of differentiation (CD)31 and CD34 in 61 cases of surgical resection of gastric carcinoma tissues, and the association between the expression of EPCR protein and MVD was analyzed. In addition, to analyze the effect of EPCR expressed by gastric cancer cells on the proliferation, migration and angiogenic abilities of endothelial cells, human umbilical vein endothelial cells (HUVECs) were cultured with tumor-conditioned medium derived from EPCR knockdown or protease-activated receptor 1 (PAR1)-blocked MGC803 gastric cancer cells. A CCK-8 assay was used to assess the proliferation ability of the HUVECs. A Transwell assay was performed to assess the migration ability of the HUVECs and a Matrigel-based tube formation assay was used to assess the angiogenic activity of the HUVECs. The results demonstrated that the expression of EPCR was correlated with the MVD of gastric cancer tissues. When cultured with tumor-conditioned medium derived from EPCR knockdown or PAR1-blocked MGC803 cells, the proliferation, migration and tubules formation abilities of HUVECs were markedly inhibited markedly. The expression of phosphorylated (p)-extracellular signal regulated kinase 1/2, p-protein kinase B (AKT; s473) and p-AKT (T308) in the HUVECs was decreased. In addition, EPCR knockdown inhibited PAR1 activation in the MGC803 cells. These results indicated that the expression of EPCR in gastric cancer cell line MGC803 contributes to tumor angiogenesis in vitro by activating ERK1/2 and AKT, and that this effect of EPCR is dependent on PAR1 activation.

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A Cell ELISA for the quantification of MUC1 mucin (CD227) expressed by cancer cells of epithelial and neuroectodermal origin

Cited by 23 publications

References 36 publications

Predictive model for risk of gastric cancer using genetic variants from genome‐wide association studies and high‐evidence meta‐analysis

Predictive model for risk of gastric cancer using genetic variants from genome‐wide association studies and high‐evidence meta‐analysis

A New Nanomaterial Based Biosensor for MUC1 Biomarker Detection in Early Diagnosis, Tumor Progression and Treatment of Cancer

EPCR promotes MGC803 human gastric cancer cell tumor angiogenesis in�vitro through activating ERK1/2 and AKT in a PAR1‑dependent manner

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