A case of mycobacteriosis in farmed pikeperch (
            <i>Sander lucioperca</i>
            ) cultured in a recirculating aquaculture system

Gjurčević, Emil; Kužir, Snježana; Žmak, Ljiljana; Obrovac, Mihaela; Kurilj, Andrea Gudan; Savoca, Serena; Pađen, Andrea; Matanović, Krešimir

doi:10.1111/are.14818

Cited by 7 publications

(6 citation statements)

References 22 publications

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“…Acidfast bacteria were commonly localized inside the granulomas. These findings were in accordance with the findings of previous studies (Gjurčević et al, 2020).…”

Section: Discussionsupporting

confidence: 94%

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Mycobacteriosis in an appearantly healty atlantic mackerel (Scomber Scombrus, L.) and zoonotic potential

Özmen

2022

Arq. Bras. Med. Vet. Zootec.

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Mycobacteriosis was detected in seven out of one Atlantic mackerel (Scomber scombrus) that was purchased for human consumption from a fish market. The fish was apparently healthy but during cleaning, several granulomatous foci were noticed in the visceral organs. Histopathological examination of the lesions revealed numerous foci characterized by caseous necrosis in the center of the lesion surrounded by epithelioid giant cells. Ziehl-Neelsen staining revealed the presence of rod-shaped, acid-fast bacteria. Furthermore, immunohistochemical examination revealed the presence of a protein of mycobacterial origin in giant cells and macrophages. Based on gross and microscopic findings, mycobacteriosis was diagnosed. This report showed that due to its zoonotic potential, mycobacteriosis should be considered even in healthy-appearing fishes for human consumption.

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“…Acidfast bacteria were commonly localized inside the granulomas. These findings were in accordance with the findings of previous studies (Gjurčević et al, 2020).…”

Section: Discussionsupporting

confidence: 94%

“…In this disease, agents cause numerous distinctive chronic granulomatous foci. Moreover, skin lesions resemble those found in other organs (Gjurčević et al, 2020). In this study, there were no lesions in skin and the fish was apparently healthy.…”

Section: Mycobacterial Infections Have Been Occasionallysupporting

confidence: 59%

Mycobacteriosis in an appearantly healty atlantic mackerel (Scomber Scombrus, L.) and zoonotic potential

Özmen

2022

Arq. Bras. Med. Vet. Zootec.

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“…Similar findings were found in striped bass [47], half-smooth tongue sole [40], hybrid striped bass [44], Atlantic cod [45], and freshwater ornamental fish infected with mycobacteria [48]. The structure of granulomas in farmed pikeperch is poorly formed with a lack of fibers [49], representing a relatively early stage of granuloma development. Meanwhile, fish granuloma is caused by a variety of pathogens, and the morphological differences between different fish species and different pathogens are not clear.…”

Section: Discussionsupporting

confidence: 65%

A Case of Mycobacteriosis in Cultured Japanese Seabass (Lateolabrax japonicus) in Southern China

Huang

Yang

et al. 2023

Fishes

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Japanese seabass (Lateolabrax japonicus) is an important species of cultured marine fish with high economic value in China. Nevertheless, from May to November 2019, mass mortality among cultured Japanese seabass occurred in Zhuhai City, Guangdong Province of China. Approximately 0.2–0.5% mortality was recorded daily, and the cumulative mortality was up to 30% during this disease outbreak. In this study, the clinical signs and pathological characteristics of diseased fish were investigated. Furthermore, the pathogenicity and antibiotic sensitivity of identified pathogenic bacteria from diseased fish were analyzed. The infected fish showed clinical signs of uncoordinated swimming; anorexia; pigment changes; and a number of 1–5 mm grayish-white nodules in the liver, spleen, and kidney tissues was also found. A bacterial strain, which was designated as ZHLJ2019, was isolated from the diseased fish. To ensure that ZHLJ2019 isolate was the causative agent, a Koch postulate trial was performed. Healthy Japanese seabass were infected by the intraperitoneal injection of 5 × 104, 5 × 105 and 5 × 106 CFU/fish, and cumulative mortalities within 42 days were 75%, 90%, and 100%, respectively. The bacteria colony had traditional morphological and biochemical characteristics similar to that of Mycobacterium marinum. Phylogenetic molecular analyses of 16S rRNA, rpoB, hsp65, erp, and ITS genes confirmed that the isolated strain ZHLJ2019 was M. marinum. The granulomatous inflammation in internal organs of Japanese seabass naturally and experimentally infected with ZHLJ2019 isolated was consistent with the classic pathological features of mycobacteriosis. Drug susceptibility of ZHLJ2019 isolate to 11 antibiotics was determined by broth dilution method in vitro. The minimum inhibitory concentrations (MICs) of minocycline, rifampicin, ethambutol, isoniazid, streptomycin, doxycycline hydrochloride, kanamycin sulfate, levofloxacin, roxithromycin, and prothionamide against the strain ZHLJ2019 were 4, 2, 8, 4, 16, 8, 8, 8, 4, and 8 μg/mL, respectively. The results of this study suggest that M. marinum is the causal agent responsible for the morbidity and mortality of Japanese seabass cultured in intensive brackish water dirt ponds in southern China.

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“…In natural and experimental infection due to bacterial and particularly mycobacterial and oomycetes species, granulomas have been described as a core of macrophages and bacteria associated with central necrosis, surrounded by a fibrous layer mostly evident in the later stages of infective granulomas [ 19 , 20 , 21 ]. Unlike mammals, in fish, the presence of giant cells has been only occasionally described in parasite granuloma [ 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Stages of Granulomatous Response Against Histozoic Metazoan Parasites in Mullets (Osteichthyes: Mugilidae)

Polinas

Padrós

Merella

et al. 2021

Animals

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Histozoic parasite–fish host interaction is a dynamic process that leads to the formation of a granuloma, a specific chronic inflammatory response with discernible histological features. Mullets (Osteichthyes: Mugilidae) represent a suitable model concerning the development of such lesions in the host–parasite interface. The present work aimed to identify granuloma developmental stages from the early to the late phase of the infection and to characterize the immune cells and non-inflammatory components of the granuloma in different stages. For this purpose, 239 mullets were collected from 4 Sardinian lagoons, and several organs were examined by combining histopathological, bacteriological, and immunohistochemical methods. Granulomas associated with trematode metacercariae and myxozoan parasites were classified into three developmental stages: (1) pre-granuloma stage, characterized by intact encysted parasite and with no or mild tissue reaction; (2) intermediate stage, with partially degenerated parasites, necrosis, and a moderate number of epithelioid cells (ECs); and (3) late stage, with a necrotic core and no detectable parasite with a high number of ECs and fibroblasts. The three-tier staging and the proposed morphological diagnosis make it conceivable that histopathology could be an essential tool to evaluate the granulomas associated with histozoic parasitic infection in fish.

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A case of mycobacteriosis in farmed pikeperch ( Sander lucioperca ) cultured in a recirculating aquaculture system

Abstract: How to cite this article: Gjurčević E, Kužir S, Žmak L, et al. A case of mycobacteriosis in farmed pikeperch (Sander lucioperca) cultured in a recirculating aquaculture system. Aquac Res.

Cited by 7 publications

References 22 publications

Mycobacteriosis in an appearantly healty atlantic mackerel (Scomber Scombrus, L.) and zoonotic potential

Mycobacteriosis in an appearantly healty atlantic mackerel (Scomber Scombrus, L.) and zoonotic potential

A Case of Mycobacteriosis in Cultured Japanese Seabass (Lateolabrax japonicus) in Southern China

Stages of Granulomatous Response Against Histozoic Metazoan Parasites in Mullets (Osteichthyes: Mugilidae)

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