A case of eumycetoma due to Madurella grisea in northern Brazil

Vilela, Raquel; Duarte, Olga M. V.; Rosa, Carlos A.; Castro, J. González; Lyon, Sandra; Motta, Rodrigo Patto Sá; Moura, Ana C. L.

doi:10.1007/s11046-004-2844-y

Cited by 9 publications

(4 citation statements)

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“…In view of the wide polymorphism which Madurella species often showed, species differentiation of Madurella can be complemented by differences in sugar assimilation and optimal growth temperature (4,14). M. mycetomatis grows well at 37°C and assimilates lactose but not sucrose, whereas M. grisea stops growth at 37°C and assimilates sucrose but not lactose (4,25). Our results indicated that strain TMMU3956 could tolerate 42°C but assimilates neither sucrose nor lactose.…”

Section: Vol 48 2010mentioning

confidence: 67%

“…Morphologically, strain TMMU3956 is more similar to M. mycetomatis than M. grisea (4,25), but in most cases, TMMU3956 formed granularis rather than woolly colonies and grew most slowly. In view of the wide polymorphism which Madurella species often showed, species differentiation of Madurella can be complemented by differences in sugar assimilation and optimal growth temperature (4,14).…”

Section: Vol 48 2010mentioning

confidence: 95%

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Phenotypic and Molecular Characterization ofMadurella pseudomycetomatissp. nov., a Novel Opportunistic Fungus Possibly Causing Black-Grain Mycetoma

et al. 2010

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A case of black-grain mycetoma occurring on the lower jaw with an odontogenic origin, which to our knowledge is the first case reported in China, is presented here. The clinical manifestation, histopathological morphology, and microbiological features are described. The new species, Madurella pseudomycetomatis, isolated from the black grains discharged by this patient, was analyzed using sequence data of the multiloci of ribosomal DNA (rDNA) and its ability to ferment carbohydrate as well as morphology. The analyses of the internal transcribed spacer (ITS) region and the D1/D2 hypervariable region of the 28S ribosomal gene sequences support a new species designation. Antifungal susceptibility testing was conducted, indicating that Madurella pseudomycetomatis was highly susceptible to itraconazole, voriconazole, and amphotericin B; moderately susceptible to terbinafine; and resistant to fluconazole and flucytosine.

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Section: Vol 48 2010mentioning

confidence: 67%

Section: Vol 48 2010mentioning

confidence: 95%

Phenotypic and Molecular Characterization ofMadurella pseudomycetomatissp. nov., a Novel Opportunistic Fungus Possibly Causing Black-Grain Mycetoma

et al. 2010

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“…Reported cases of mycetoma caused by T. grisea originated from South America and India. Unconfirmed reports were published from North and Central America, Africa, and Asia ( Green & Adams 1964 , Butz & Ajello 1970 , Mahgoub 1973 , Venugopal et al 1990 , Vilela et al 2004 ). In most of these publications, identification was based on morphological or physiological properties and may have been misidentifications ( Desnos-Ollivier et al 2006 ).…”

Section: Discussionmentioning

confidence: 94%

Revision of agents of black-grain eumycetoma in the order <I>Pleosporales</I>

Ahmed¹,

Sande²,

Stevens³

et al. 2014

Pers - Int Mycol J

103

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Eumycetoma is a chronic fungal infection characterised by large subcutaneous masses and the presence of sinuses discharging coloured grains. The causative agents of black-grain eumycetoma mostly belong to the orders Sordariales and Pleosporales. The aim of the present study was to clarify the phylogeny and taxonomy of pleosporalean agents, viz. Madurella grisea, Medicopsis romeroi (syn.: Pyrenochaeta romeroi), Nigrograna mackinnonii (syn. Pyrenochaeta mackinnonii), Leptosphaeria senegalensis, L. tompkinsii, and Pseudochaetosphaeronema larense. A phylogenetic analysis based on five loci was performed: the Internal Transcribed Spacer (ITS), large (LSU) and small (SSU) subunit ribosomal RNA, the second largest RNA polymerase subunit (RPB2), and translation elongation factor 1-alpha (TEF1) gene. In addition, the morphological and physiological characteristics were determined. Three species were well-resolved at the family and genus level. Madurella grisea, L. senegalensis, and L. tompkinsii were found to belong to the family Trematospheriaceae and are reclassified as Trematosphaeria grisea comb. nov., Falciformispora senegalensis comb. nov., and F. tompkinsii comb. nov. Medicopsis romeroi and Pseudochaetosphaeronema larense were phylogenetically distant and both names are accepted. The genus Nigrograna is reduced to synonymy of Biatriospora and therefore N. mackinnonii is reclassified as B. mackinnonii comb. Nov. Mycetoma agents in Pleosporales were phylogenetically quite diverse despite their morphological similarity in the formation of pycnidia, except for the ascosporulating genus Falciformispora (formerly in Leptosphaeria). Most of the species diagnosed from human mycetoma were found to be related to waterborne or marine fungi, suggesting an association of the virulence factors with oligotrophism or halotolerance.

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“…From 1980 to 2014, the main bacterial agents identified in Brazil were Nocardia brasiliensis [15,26,27–32], Nocardia asteroides [15,33], Nocardia caviae [34], Actinomadura madurae [13,35,36], Actinomadura pelletieri [14], and Streptomyces somaliensis [15]. For eumycetoma were Madurella mycetomatis [15,25,37,38], Madurella grisea [39–45], Acremonium falciforme [46], Acremonium kiliense [47], Scedosporium apiospermum [12,18,48,49,50], Fusarium solani [51], Exophiala jeanselmei [44,52,53] and Aspergillus sp. [12].…”

Section: Discussion and Review Of The Literaturementioning

confidence: 99%

Review of 21 cases of mycetoma from 1991 to 2014 in Rio de Janeiro, Brazil

et al. 2017

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Mycetoma is caused by the subcutaneous inoculation of filamentous fungi or aerobic filamentous bacteria that form grains in the tissue. The purpose of this study is to describe the epidemiologic, clinic, laboratory, and therapeutic characteristics of patients with mycetoma at the Oswaldo Cruz Foundation in Rio de Janeiro, Brazil, between 1991 and 2014. Twenty-one cases of mycetoma were included in the study. There was a predominance of male patients (1.3:1) and the average patient age was 46 years. The majority of the cases were from the Southeast region of Brazil and the feet were the most affected anatomical region (80.95%). Eumycetoma prevailed over actinomycetoma (61.9% and 38.1% respectively). Eumycetoma patients had positive cultures in 8 of 13 cases, with isolation of Scedosporium apiospermum species complex (n = 3), Madurella mycetomatis (n = 2) and Acremonium spp. (n = 1). Two cases presented sterile mycelium and five were negative. Six of 8 actinomycetoma cases had cultures that were identified as Nocardia spp. (n = 3), Nocardia brasiliensis (n = 2), and Nocardia asteroides (n = 1). Imaging tests were performed on all but one patients, and bone destruction was identified in 9 cases (42.68%). All eumycetoma cases were treated with itraconazole monotherapy or combined with fluconazole, terbinafine, or amphotericin B. Actinomycetoma cases were treated with sulfamethoxazole plus trimethoprim or combined with cycles of amikacin sulphate. Surgical procedures were performed in 9 (69.2%) eumycetoma and in 3 (37.5%) actinomycetoma cases, with one amputation case in each group. Clinical cure occurred in 11 cases (7 for eumycetoma and 4 for actinomycetoma), and recurrence was documented in 4 of 21 cases. No deaths were recorded during the study. Despite of the scarcity of mycetoma in our institution the cases presented reflect the wide clinical spectrum and difficulties to take care of this neglected disease.

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A case of eumycetoma due to Madurella grisea in northern Brazil

Cited by 9 publications

References 4 publications

Phenotypic and Molecular Characterization ofMadurella pseudomycetomatissp. nov., a Novel Opportunistic Fungus Possibly Causing Black-Grain Mycetoma

Phenotypic and Molecular Characterization ofMadurella pseudomycetomatissp. nov., a Novel Opportunistic Fungus Possibly Causing Black-Grain Mycetoma

Revision of agents of black-grain eumycetoma in the order <I>Pleosporales</I>

Review of 21 cases of mycetoma from 1991 to 2014 in Rio de Janeiro, Brazil

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