A candidate reference method for uric acid in serum. I. Optimization and evaluation.

Duncan, P H; Gochman, Nathan; Cooper, T.R.; Smith, Elizabeth; Bayse, D D

doi:10.1093/clinchem/28.2.284

Cited by 43 publications

(6 citation statements)

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“…In brief: uric acid present in the sample was converted by uricase into allantoin and hydrogen peroxide. The latter is then catalyzed by peroxidase present in the reagent mixture and oxidizes aminobenzene to produce red quinoneimine compound that absorbs at 520 nm 35 …”

Section: Methodsmentioning

confidence: 99%

Alterations in Salivary Antioxidants, Nitric Oxide, and Transforming Growth Factor‐β₁ in Relation to Disease Activity in Crohn's Disease Patients

Rezaie¹,

Ghorbani²,

Eshghtork³

et al. 2006

Annals of the New York Academy of Sciences

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It has been postulated that oxidative stress, nitric oxide (NO), and transforming growth factor beta(1) (TGF- beta(1)) have major roles in the pathophysiology of Crohn's disease (CD). The aim of this study was to determine the salivary levels of total antioxidant capacity (TAC), specific antioxidants (i.e., uric acid, albumin, transferrin, and thiol molecules), lipid peroxidation (LPO), NO, and TGF- beta(1) in CD patients and control subjects and to also investigate their correlation with activity of the disease. Twenty-eight patients with confirmed diagnosis of CD were enrolled and whole saliva samples were obtained. Smokers, diabetics, those who suffered from periodontitis, and those who were consuming antioxidant supplements were excluded from the study. The Crohn's Disease Activity Index (CDAI) was used to determine the severity of the disease. Twenty healthy subjects were also recruited. In CD patients significant reductions in salivary levels of TAC (0.248 +/- 0.145 vs. 0.342 +/- 0.110 mmol/L), albumin (1.79 +/- 0.42 vs. 2.3 +/- 0.2 microg/mL), and uric acid (3.1 +/- 1.4 vs. 4.1 +/- 2.0 mg/dL) were found. TGF-beta(1) was significantly increased in CD patients compared to healthy subjects (3.02 +/- 1.54 vs. 2.36 +/- 0.52 ng/mL). A fourfold increase in NO levels (198.8 +/- 39.9 vs. 50.2 +/- 21.3 micromol/L) along with a fivefold increase in LPO concentration (0.146 +/- 0.064 vs. 0.027 +/- 0.019 micromol/L) was documented in CD patients in comparison to the control group. CDAI significantly correlated with the TAC, LPO, and the interaction between TAC and LPO (r(2) = 0.625, r(2) = 0.8, F-test's P < 0.00005). Saliva of CD patients exhibits an abnormal feature with respect to oxidative stress, NO, and TGF-beta(1). TAC and LPO modify the effect of each other in determination of CD severity, which underlines the importance of oxidative stress in the pathogenesis of CD.

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Section: Methodsmentioning

confidence: 99%

Alterations in Salivary Antioxidants, Nitric Oxide, and Transforming Growth Factor‐β₁ in Relation to Disease Activity in Crohn's Disease Patients

Rezaie¹,

Ghorbani²,

Eshghtork³

et al. 2006

Annals of the New York Academy of Sciences

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“…by incubating urine samples with uricase. The The classical method for uric analysis uses highly thymol / thimerosal peak appeared at 31 min during purified microbial uricase to quantitate uric acid by the washing step and did not interfere with the differential ultraviolet procedure [68].…”

Section: In Relation To Sample Storage High Urinary Uricmentioning

confidence: 99%

Urinary analysis of nephrolithiasis markers

Barbas

Garcı́a

Saavedra

et al. 2002

Journal of Chromatography B

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Renal stone disease is an ancient and common affliction, common in industrialised nations. The causes and incidence of nephrolithiasis are presented. Afterwards, the promoters and inhibitors of renal stone formation analysis in urine are described including enzymatic methods, chromatography, capillary electrophoresis and other techniques. Aspects such as sample collection and storage are also included. The review article includes referenced tables that provide summaries of methodology for the analysis of nephrolithiasis related compounds.

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“…Different reference methods have been established in order to provide a reliable standard and to improve the accuracy of UA measurement in serum ( Table 1 ) [ 9 - 15 ]. In the present work, we searched for a simple, rapid, and economical reference method that also had good analytical performance.…”

Section: Introductionmentioning

confidence: 99%

“…In the present work, we searched for a simple, rapid, and economical reference method that also had good analytical performance. Duncan et al [ 9 ] optimized and evaluated a manual UV uricase method for measuring UA. This method exhibited satisfactory analytical recoveries (98-105%) and precision (day-to-day precision, 1.5% [590 µmol/L] to 2.7% [180 µmol/L]) and was resistant to endogenous interference, including ascorbate and glutathione levels.…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Trueness and Inter-Laboratory Precision of Routine Uric Acid Assays Using 4 Frozen Pooled Serum Samples Measured by the Japan Society of Clinical Chemistry's HPLC Method

Jiang

2014

Ann Lab Med

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BackgroundReference procedures are required for evaluating the accuracy of routine analytical systems for uric acid (UA). External quality assessment (EQA) for UA has only been conducted with quality controls in China, and the results have not been published. This study was designed to investigate both the trueness and inter-laboratory precision of UA measurements among routine analytical systems using a candidate reference method.MethodsWe performed the HPLC method recommended by the Japan Society of Clinical Chemistry (JSCC). Next, we evaluated its analytical performance and validated its trueness. The performance of 4 routine analytical systems (5 instruments per system, n=20) for UA was assessed by using 4 frozen pooled serum samples measured by the HPLC method according to biologically relevant quality goals.ResultsWithin-run, between-run, inter-day, and total CV of the method were less than 0.3%, 0.4%, 1.8%, and 2.6%, respectively. The UA measurements were consistent with the target values of standard reference material (SRM) 909b, the sixth ring trial for Reference Laboratories (RELA-2008) specimen, and national primary reference materials. The 4 frozen pooled serum samples were homogeneous, stable, and commutable. All routine systems achieved the desirable performance goal (total error <11.9%).ConclusionsWe successfully reproduced the JSCC's HPLC method, which was simple, specific, precise, and accurate. We recommend this method as a reference method for UA measurement in human serum. Four routine analytical systems for UA measurement had acceptable traceability, and their UA results showed good concordance.

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A candidate reference method for uric acid in serum. I. Optimization and evaluation.

Cited by 43 publications

References 0 publications

Alterations in Salivary Antioxidants, Nitric Oxide, and Transforming Growth Factor‐β₁ in Relation to Disease Activity in Crohn's Disease Patients

Alterations in Salivary Antioxidants, Nitric Oxide, and Transforming Growth Factor‐β₁ in Relation to Disease Activity in Crohn's Disease Patients

Urinary analysis of nephrolithiasis markers

Assessment of the Trueness and Inter-Laboratory Precision of Routine Uric Acid Assays Using 4 Frozen Pooled Serum Samples Measured by the Japan Society of Clinical Chemistry's HPLC Method

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A candidate reference method for uric acid in serum. I. Optimization and evaluation.

Cited by 43 publications

References 0 publications

Alterations in Salivary Antioxidants, Nitric Oxide, and Transforming Growth Factor‐β1 in Relation to Disease Activity in Crohn's Disease Patients

Alterations in Salivary Antioxidants, Nitric Oxide, and Transforming Growth Factor‐β1 in Relation to Disease Activity in Crohn's Disease Patients

Urinary analysis of nephrolithiasis markers

Assessment of the Trueness and Inter-Laboratory Precision of Routine Uric Acid Assays Using 4 Frozen Pooled Serum Samples Measured by the Japan Society of Clinical Chemistry's HPLC Method

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Product

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Alterations in Salivary Antioxidants, Nitric Oxide, and Transforming Growth Factor‐β₁ in Relation to Disease Activity in Crohn's Disease Patients

Alterations in Salivary Antioxidants, Nitric Oxide, and Transforming Growth Factor‐β₁ in Relation to Disease Activity in Crohn's Disease Patients