A biosensor based on cytochrome c immobilization on a poly-3-methylthiophene/multi-walled carbon nanotubes hybrid-modified electrode. Application to the electrochemical determination of nitrite

Eguílaz, Marcos; Agüı́, L.; Yáñez‐Sedeño, Paloma; Pingarrón, José M.

doi:10.1016/j.jelechem.2010.03.025

Cited by 79 publications

(30 citation statements)

References 40 publications

(49 reference statements)

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“…In most cases, polypyrrole and polythiophene films serve merely as the matrix to embed nanostructures (gold nanoparticles, carbon nanotubes, graphene, etc.) that are employed for DET to proteins in sensor applications [43][44][45][46] . Little number of fundamental studies based exclusively on conducting polymers (i.e.…”

Section: Introductionmentioning

confidence: 99%

Direct Electron Transfer to Cytochrome c Induced by a Conducting Polymer

et al. 2017

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The direct electron transfer (DET) to redox proteins has become a central topic in the development of biotechnological devices. The present work explores the mechanisms of the direct electrochemistry between cytochrome c and a conducting polymer, PEDOT-PSS. This polymer has been electrosynthesized from its monomers in aqueous solution on gold electrodes and its capabilities for DET to cyt c have been examined by electrochemical and spectroelectrochemical methods. The polymer was electrodeposited with controlled thickness and we determined the electron transfer rate constant for cyt c oxidation was about 2 orders of magnitude higher than those obtained at conventional electrodes. Spectroelectrochemical measurements allowed to evaluate the redox state of the polymer as a function of the potential and, in addition, the observation of intrinsic cyt c redox activity upon electron transfer from the conducting polymer. During the oxidation process of this protein, lysine residues placed near the heme crevice interact electrostatically with the anionic polyelectrolyte PSS. This interaction favors the orientation of the heme group towards the chains of PEDOT backbone, which is the eventual responsible for the electron transfer to the protein.3

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Section: Introductionmentioning

confidence: 99%

Direct Electron Transfer to Cytochrome c Induced by a Conducting Polymer

et al. 2017

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“…A lot of synthetic methods have been used to synthesize polythiophene derivatives (PTs), for example, nickel-mediated cross-coupling reactions30, palladium-catalyzed cross-coupling reactions29, oxidative polymerization31, electrochemical polymerization32, and biocatalyzed polymerization33. Oxidative polymerization catalyzed by FeCl 3 is one of the favored methods among the aforementioned synthetic methods to afford WSPTs because of the ease of this reaction.…”

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confidence: 99%

Synthesis and Characterization of Water-Soluble Polythiophene Derivatives for Cell Imaging

Wang

et al. 2015

Sci Rep

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In this work, four water-soluble polythiophene derivatives (PT, PT-DDA, PT-ADA, and PT-ADA-PPR) with different pendant moieties were synthesized via oxidative copolymerization by FeCl3. By increasing the hydrophobic ability of side chain moieties, there is a gradually blue shift for the maximum absorption wavelength and red shift for the maximum emission wavelength, a reducing trend for fluorescence quantum yields, a growing trend for Stokes shift, and an increasing trend for the mean sizes in the order of PT, PT-ADA, and PT-DDA. All the synthesized polymers show low toxicity and good photostability and accumulate in the lysosomes of A549 cells. Furthermore, the introduction of porphyrin group to PT-ADA side chain (PT-ADA-PPR) broadens the absorption and emission ranges of PT-ADA. PT-ADA-PPR could be excited at two different excitation wavelengths (488 nm and 559 nm) and exhibits two emission pathways, and dual-color fluorescence images (orange and red) of PT-ADA-PPR accumulated in A549 cells are observed. Thus, PT-ADA-PPR could be used as an excellent dual-color fluorescent and lysosome-specific imaging material.

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“…Recently, many kinds of NO 2 ‐ biosensors have been fabricated including codeposited Au‐Fe(III) nanoparticles, Au nanoparticles and choline chloride hybrid, DNA functional carbon nanocubes/Cu 2+ complex, and immobilizing cytochrome c on P3MT/MWCNTs hybrid. Most NO 2 ‐ biosensors were attributed to the catalysis of proteins for the reductive reaction of NO 2 ‐ . But the electrochemical reduction of NO 2 ‐ would produce several products depending on the electrode and the catalyst, however, its oxidation was a straightforward reaction with nitrate being the final produce.…”

Section: Introductionmentioning

confidence: 99%

Amperometric sensor for nitrite using a glassy carbon electrode modified with thionine functionalized MWCNTs/Au nanorods/SDS nanohybrids

Zhang

Yuan

Zhong

et al. 2012

Surface & Interface Analysis

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A sensitive nitrite (NO 2 -) biosensor was fabricated by using sodium dodecyl sulfate (SDS), Au nanorods, and thionine functionalized MWCNTs (TH-f-MWCNTs) nanohybrids modified glassy carbon electrode. TH was covalently immobilized on the MWCNTs via a carbodiimide reaction. Comparing with MWCNTs/GCE, TH-f-MWCNTs/GCE displays higher catalytic activity toward the oxidation of NO 2 -, since TH not only promoted the electronic transmission but also could improve the concentration of NO 2 at the surface of the modified electrode in acidic solutions. The Au nanorods (AuNRs) were prepared through a simple wet chemical method and were characterized by TEM. The extremely high surface-to-volume ratios associated with one dimension nanostructures make their electrical properties extremely sensitive to species adsorbed on surfaces and result in excellent sensitivity and selectivity. SDS displays excellent film forming ability, which made the electrode stable. Under optimal conditions, the linear range for the detection of nitrite was 0.26 to 51 mM, and the low detection limit was 20 nM. In addition, the modified electrode was successfully applied to determine nitrite in real water samples.

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A biosensor based on cytochrome c immobilization on a poly-3-methylthiophene/multi-walled carbon nanotubes hybrid-modified electrode. Application to the electrochemical determination of nitrite

Cited by 79 publications

References 40 publications

Direct Electron Transfer to Cytochrome c Induced by a Conducting Polymer

Direct Electron Transfer to Cytochrome c Induced by a Conducting Polymer

Synthesis and Characterization of Water-Soluble Polythiophene Derivatives for Cell Imaging

Amperometric sensor for nitrite using a glassy carbon electrode modified with thionine functionalized MWCNTs/Au nanorods/SDS nanohybrids

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