A Bioorthogonal Raman Reporter Strategy for SERS Detection of Glycans on Live Cells

Lin, Liang; Tian, Xiangdong; Hong, Senlian; Dai, Peng; You, Qiancheng; Wang, Ruyi; Feng, Lian‐Shun; Xie, Can; Zhang, Tian; Chen, Xing

doi:10.1002/anie.201301387

Cited by 135 publications

(106 citation statements)

References 29 publications

Supporting

Mentioning

104

Contrasting

Order By: Relevance

“…Another series of small chemical groups, like alkyne, nitrile, azide, and deuterium, are expected to show Raman scattering in the cellular silent region as well. 334 Among these groups, alkyne has clear advantages over the other three candidates in terms of high signal intensity, narrow line width, and suitable wavenumber of the signal. By combining AuNP-based SERS and a biorthogonal Raman reporter, the glycans on the membrane of living cells were measured.…”

Section: Aunp-based Sers Assaysmentioning

confidence: 99%

Gold Nanoparticles for In Vitro Diagnostics

et al. 2015

View full text Add to dashboard Cite

show abstract

Section: Aunp-based Sers Assaysmentioning

confidence: 99%

Gold Nanoparticles for In Vitro Diagnostics

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Cyanide bands at ca. 2100 cm −1 have been utilized as a marker band because of their discriminating position in the vibrational spectral window without the interference of multiple overlapping bands between 1000 and 1700 cm −1 [21,22]. In view of such electrochemical reactions [20], GLY may provide a unique marker of the cyanide group on metal surfaces.…”

Section: Introductionmentioning

confidence: 99%

Detection of Copper(II) Ions Using Glycine on Hydrazine-Adsorbed Gold Nanoparticles via Raman Spectroscopy

Seo

Joo

2016

Sensors

View full text Add to dashboard Cite

A facile, selective, and sensitive detection method for the Cu2+ ions in environmental and biological solutions has been newly developed by observing the unique CN stretching peaks at ~2108 cm−1 upon the dissociative adsorption of glycine (GLY) in hydrazine buffer on gold nanoparticles (AuNPs). The relative abundance of Cu species on AuNPs was identified from X-ray photoelectron spectroscopy analysis. UV-Vis spectra also indicated that the Au particles aggregated to result in the color change owing to the destabilization induced by the GLY-Cu2+ complex. The CN stretching band at ~2108 cm−1 could be observed to indicate the formation of the CN species from GLY on the hydrazine-covered AuNP surfaces. The other ions of Fe3+, Fe2+, Hg2+, Mg2+, Mn2+, Ni2+, Zn2+, Cr3+, Co2+, Cd2+, Pb2+, Ca2+, NH4+, Na+, and K+ at high concentrations of 50 µM did not produce such spectral changes. The detection limit based on the CN band for the determination of the Cu2+ ion could be estimated to be as low as 500 nM in distilled water and 1 µM in river water, respectively. We attempted to apply our method to estimate intracellular ion detection in cancer cells for more practical purposes.

show abstract

“…16,17 Recently, the unique Raman peaks (2000–2800 cm −1 ) of the azido, alkynyl and cyano groups have been explored as Raman tags for cellular imaging. 18,19 Detection of SERS from nucleotide bases has been proposed to determine DNA sequences 20,21 but no successful examples have been published.…”

Section: Introductionmentioning

confidence: 99%

DNA sequencing by synthesis using 3′-O-azidomethyl nucleotide reversible terminators and surface-enhanced Raman spectroscopic detection

et al. 2014

View full text Add to dashboard Cite

As an alternative to fluorescence-based DNA sequencing by synthesis (SBS), we report here an approach using an azido moiety (N3) that has an intense, narrow and unique Raman shift at 2125 cm−1, where virtually all biological molecules are transparent, as a label for SBS. We first demonstrated that the four 3′-O-azidomethyl nucleotide reversible terminators (3′-O-azidomethyl-dNTPs) displayed surface enhanced Raman scattering (SERS) at 2125 cm−1. Using these 4 nucleotide analogues as substrates, we then performed a complete 4-step SBS reaction. We used SERS to monitor the appearance of the azide-specific Raman peak at 2125 cm−1 as a result of polymerase extension by a single 3′-O-azidomethyl-dNTP into the growing DNA strand and disappearance of this Raman peak with cleavage of the azido label to permit the next nucleotide incorporation, thereby continuously determining the DNA sequence. Due to the small size of the azido label, the 3′-O-azidomethyl-dNTPs are efficient substrates for the DNA polymerase. In the SBS cycles, the natural nucleotides are restored after each incorporation and cleavage, producing a growing DNA strand that bears no modifications and will not impede further polymerase reactions. Thus, with further improvements in SERS for the azido moiety, this approach has the potential to provide an attractive alternative to fluorescence-based SBS.

show abstract

A Bioorthogonal Raman Reporter Strategy for SERS Detection of Glycans on Live Cells

Cited by 135 publications

References 29 publications

Gold Nanoparticles for In Vitro Diagnostics

Gold Nanoparticles for In Vitro Diagnostics

Detection of Copper(II) Ions Using Glycine on Hydrazine-Adsorbed Gold Nanoparticles via Raman Spectroscopy

DNA sequencing by synthesis using 3′-O-azidomethyl nucleotide reversible terminators and surface-enhanced Raman spectroscopic detection

Contact Info

Product

Resources

About