A biomimetic enzyme modified electrode for H₂O₂ highly sensitive detection

Abstract

An efficient catalyst based on artificial bionic peroxidase was synthesized for electrocatalysis. A poly(ethyleneimine)/Au nanoparticle composite (PEI-AuNP) was prepared and it was then linked to hemin via a coupling reaction between carboxyl groups in hemin and amino groups in PEI without the activation of a carboxyl group by carbodiimide. Fourier transform infrared (FTIR) spectroscopy verified the formation of amido bonds within the structure. The presence of AuNPs contributed greatly in establishing the amido bonds within the composite. Transmission electron microscopy (TEM) and UV-visible spectroscopy were also used to characterize the PEI-AuNP-hemin catalyst. PEI-AuNP-hemin exhibited intrinsic peroxidase-like catalytic activities. The PEI-AuNP-hemin deposited on a glass carbon electrode had strong sensing for H2O2 with a well-defined linear relationship between the amperometric response and H2O2 concentration in the range from 1 μM to 0.25 mM. The detection limit was 0.247 nM with a high sensitivity of 0.347 mA mM(-1) cm(-2). The peroxidase-like catalytic activity of PEI-AuNP-hemin is discussed in relation to its microstructure. The study suggests that PEI-AuNP-hemin may have promising application prospects in biocatalysis and bioelectronics.

Hemin Based Biomimetic Oxidative Degradation of Acid Orange 7

“…The order of dependence on H 2 O 2 is significantly larger than that of hemin and the result is in keeping with the highly sensitive detection for H 2 O 2 of linked hemin on the PEI-AuNP in our study [26].…”

Recent Advances on Nanozyme‐based Electrochemical Biosensors

“…Peroxidase-like nanozymes can be used as electrode modifiers for the determination of H 2 O 2 because they can catalyze the reduction of H 2 O 2 [52][53][54][55][56][57]. For example, electrochemical detection of H 2 O 2 was achieved by using Prussian Blue as an artificial peroxidase to modify a glassy carbon electrode (GCE) [53].…”

Detection Technologies for Reactive Oxygen Species: Fluorescence and Electrochemical Methods and Their Applications

“…For example, hemin was immo- Porous materials have also been used for immobilization of redox proteins. Various conductive complex materials, such as Ag-Au [162], Fe 3 O 4 -Au [150], polyethylenimine (PEI)-Au [163], and GO-chitosan-Au [159][160][161]164], have been reported in the literature. As previously mentioned, one of the main reasons for integrating redox proteins with conductive substrates with large surface areas is to improve the amount and stability of bound redox proteins on the electrode surface.…”