A biologically active fructan from the roots of Arctium lappa L., var. Herkules

Kardošová, Alžbeta; Ebringerová, Anna; Alföldi, Juraj; Nosálóvá, G; Fraňová, Soňa; Hřı́balová, Věra

doi:10.1016/s0141-8130(03)00079-5

Cited by 58 publications

(32 citation statements)

References 17 publications

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“…. This also confirmed the linear structure of the isolated polymer [17]. Thus, Sl-GF was a high-molecular-weight linear GFr of the inulin type that consisted of E-(2o1)-bonded fructofuranose units.…”

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confidence: 74%

Glucofructans from Saussurea lappa roots

2011

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MW 51.4 kDa), which was a linear inulin-type glucofructan consisting of E-(2o1)-bonded fructofuranose units, was isolated and characterized. The total content of glucofructans in Saussurea lappa roots was 476.97-578.27 mg/g. Saussurea lappa C. B. Clarke [S. costus (Falc.) Lipsch.] is a member of the Asteraceae family and is widely used as a medicinal agent in China, India, and Tibet [1]. Roots of S. lappa (muxiang, yunmuxiang) are used in traditional Chinese medicine to treat headache, loss of appetite, diarrhea, and abdominal pain [2]. S. lappa (ru rta) is used in Tibetan medical practice for stomach ulcers and lung diseases and as an antihelminthic and hemostatic agent [3]. A study of the chemical composition of the roots of this plant showed that sesquiterpenes [4-7], triterpenes [5-9], lignans [10], phenylpropanoids [10, 11], chromones [9], simple phenols and phenolic acids [9, 10], flavonoids [12], anthraquinones [11], 2-pyrrolidinon-5(s)-carboxylate, 5-hydroxymethylfurfural, succinic acid, glucose [9], and essential oil [13] in addition to inulin were present. However, the chemistry of glucofructans (GFr) from this plant species has not previously been studied [14,15]. The goal of our work was to study the composition and chemical structure of GFr from S. lappa roots.GFr were isolated as follows. Ground roots of S. lappa were treated beforehand with several solvents to remove lipophilic and phenolic compounds. The remaining raw material was extracted with EtOH (80%) and H 2 O to afford two fractions of alcohol-(SlF-1) and water-soluble GFr (SlF-2) in yields of 18.34 and 34.72% of the air-dried mass of raw material, respectively. The fructose contents in SlF-1 and SlF-2 were 67.53 ± 0.74 and 90.33 ± 1.08%, respectively.A chromatographic study (HPTLC) of fraction SlF-1 showed that it contained fructose and a series of oligomeric GFr. The latter were identified by separating SlF-1 using ion-exchange and gel-permeation chromatography. As a result, five compounds were identified using methylation as saccharose (1), 1-kestose (2), nystose (3), 1 F -E-fructofuranosylnystose (4), and 1 F -E-fructofuranosyl-1 F -E-fructofuranosylnystose (5). All compounds were typical of GFr-containing plant raw material. However, they were observed in S. lappa for the first time. Quantitative analysis using an HPTLC-densitometric method showed that the fructose content in S. lappa roots was (mg/g) 22. 91-50.15; in 1, 13.14-47.04; 2, 11.43-34.51; 3, 2.21-15.68; 4, 8.36-50.05; and 5, 6.76-22.39 (Table 1).The high fructose content and the IR spectrum of SlF-2 suggested that it contained polymeric GFr. Gel chromatography showed that SlF-2 was heterogeneous and contained compounds with molecular weights (MWs) 2.5-70 kDa. Ion-exchange chromatography over DEAE-cellulose and gel chromatographic separation over Molselect G-25 and Sephacryl S-300 HR were used to isolate the dominant polymer. As a result, homogeneous polymer Sl-GF was isolated in 24.1% yield (of SlF-2 mass). The MW of Sl-GF according to HPLC was 51.4 kDa.

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confidence: 74%

Glucofructans from Saussurea lappa roots

2011

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“…too, LF produced somewhat similar response in mitogenic activity at the same concentrations. It is worth noting here that a low molecular weight fructofuranan of the inulin-type from the roots of Arctium lappa (a Compositae member used as an edible vegetable in the Orient) was found to exhibit mitogenicity and comitogenicity comparable to that of the commercial zymosan immunomodulator (Spies et al, 1992;Kardošová et al, 2003). Further, a fructan from Radix ophiopogonis has also been shown to stimulate the proliferation of cultured lymphocytes (Wu et al, 2006).…”

Section: Mitogenic Activity Of Purified Fructansmentioning

confidence: 80%

Isolation, structural elucidation and immunomodulatory activity of fructans from aged garlic extract

2011

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“…13 C NMR spectral analysis 13 C NMR spectra of EPS-1 and EPS-2 were recorded on a Bruker AV-500 NMR spectrometer with an operating frequency of 125 MHz at 30°C for 8 h. Samples were prepared by dissolving 10 mg of EPS-1 or EPS-2 in 0.5 ml D 2 O (99.9%). The carbon signals were assigned by comparison with the chemical shifts in literature reported (Chen & Tian, 2003;Cunha, Vieira, Arriaga, de Paula, & Feitosa, 2009;Han & Clarke, 1990;Kardošová et al, 2003;Tajima et al, 1998;Wack & Blaschek, 2006).…”

Section: Methylation and Gc-ms Analysismentioning

confidence: 99%