A bio-barcode assay for on-chip attomolar-sensitivity protein detection

Goluch, Edgar D.; Nam, Jwa Min; Georganopoulou, Dimitra G.; Chiesl, Thomas N.; Shaikh, Kashan; Ryu, Kee Suk; Barron, Annelise E.; Mirkin, Chad A.; Liu, Chang

doi:10.1039/b606294f

Cited by 198 publications

(148 citation statements)

References 33 publications

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“…A recent analysis of human genomes supports this approach and identifies many possible such bar-codes in the fundamental genomic modulation in the cellular responses to viral infections [29]. Additionally, the bar-code approach is useful to identify multiple disease markers [30].…”

Section: Molecular Signature Concepts and Variationsmentioning

confidence: 92%

Untitled

2008

Bioinformation

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Abstract:We describe current research that applies epigenetics to a novel understanding of the immuno-neuropathogenesis of HIV-1 viral infection and NeuroAIDS. We propose the hypothesis that HIV-1 alters the structure-function relationship of chromatin, coding DNA and non-coding DNA, including RNA transcribed from these regions resulting in pathogenesis in AIDS, drug abuse, and NeuroAIDS. We discuss the general implications of molecular epigenetics with special emphasis on drug abuse, bar-codes, pyknons, and miRNAs for translational and clinical research. We discuss the application of the recent recursive algorithm of biology to this field and propose to synthesize the Genomic and Epigenomic views into a holistic approach of HoloGenomics.

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Section: Molecular Signature Concepts and Variationsmentioning

confidence: 92%

Untitled

2008

Bioinformation

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“…Indeed, biomolecular detection with up to attomolar resolution was achieved by combining a microfluidic device with electronic or optical signal detection [187]. Goluch developed a bio-barcode assay for single protein detection by using functionalized NPs [188]. Gong developed integrated nanoelectronic and electrokinetic devices for label-free attomolar detection of proteins [189].…”

Section: Microfluidics Systems For Single-cell Studiesmentioning

confidence: 99%

USNCTAM perspectives on mechanics in medicine

Bao

Bazilevs

Chung

et al. 2014

J. R. Soc. Interface.

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Over decades, the theoretical and applied mechanics community has developed sophisticated approaches for analysing the behaviour of complex engineering systems. Most of these approaches have targeted systems in the transportation, materials, defence and energy industries. Applying and further developing engineering approaches for understanding, predicting and modulating the response of complicated biomedical processes not only holds great promise in meeting societal needs, but also poses serious challenges. This report, prepared for the US National Committee on Theoretical and Applied Mechanics, aims to identify the most pressing challenges in biological sciences and medicine that can be tackled within the broad field of mechanics. This echoes and complements a number of national and international initiatives aiming at fostering interdisciplinary biomedical research. This report also comments on cultural/educational challenges. Specifically, this report focuses on three major thrusts in which we believe mechanics has and will continue to have a substantial impact. (i) Rationally engineering injectable nano/microdevices for imaging and therapy of disease. Within this context, we discuss nanoparticle carrier design, vascular transport and adhesion, endocytosis and tumour growth in response to therapy, as well as uncertainty quantification techniques to better connect models and experiments. (ii) Design of biomedical devices, including point-of-care diagnostic systems, model organ and multi-organ microdevices, and pulsatile ventricular assistant devices. (iii) Mechanics of cellular processes, including mechanosensing and mechanotransduction, improved characterization of cellular constitutive behaviour, and microfluidic systems for single-cell studies.

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“…Moreover, improving bead dispersion and compromising on the incubation times would increase the sensitivity of the assay significantly. Nevertheless, even with the current protocol, the dual network system is among the most sensitive microfluidic immunoassays reported to date, 3,4,[27][28][29][30][31][32][33][34][35][36][37][38] without the use of costly, complex and time-consuming pre-treatments.…”

Section: Proof-of-concept: Quantification Of a Model Antibodymentioning

confidence: 99%