A Bifidobacterium-based synbiotic product to reduce the transmission of C. jejuni along the poultry food chain

Baffoni, Loredana; Gaggìa, Francesca; Gioia, Diana Di; Santini, Cecilia; Mogna, Luca; Biavati, Bruno

doi:10.1016/j.ijfoodmicro.2012.04.024

Cited by 87 publications

(68 citation statements)

References 34 publications

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“…In this study, data from dye experiments strongly suggests that the solvent evaporation technique may be used to successfully encapsulate and release prebiotic materials at desired controlled rates into the small intestine where they are selectively fermented for the proliferation of beneficial gram positive bacteria strains. Results from this study have shown that the use of probiotics and prebiotics prepared using solvent evaporation technique have the potentials to create a variety of products in the food industry that can be used for therapeutic purposes, thus confirming recent reports (Baffoni et al,2012;Prakash et al,2011;Shaik et al,2012). The synergistic effects of both probiotics and prebiotics have the propensity to deliver delicate food ingredients to target cells in both humans and animals.…”

Section: Discussionsupporting

confidence: 89%

“…This approach has been shown to release cells at sustained and continuous rates in therapeutic products (Murua et al,2008). The use of polymer microcapsules as delivery systems has been reported and reviewed in recent literature to have desirable pharmaceutical applications (Paul et al, 2011;Baffoni et al, 2012;Shaik et al, 2012). How flexible or rigid they are plays an important role their structure-function relationship as well as their scope of application in the food and biomedical industries (Morrisa et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Preliminary studies on pharmaceutical microencapsulation for synbiotic application

Evivie

2013

JANS

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Abstract:The solvent evaporation technique was used in this study to evaluate probiotics microencapsulation in AQOAT/ dichloromethane matrix and cell viability was also measured to assess its potentials in industrial food formulation processes. Cumulative release experiments using water blue dye as a model molecule was also used to evaluate the prebiotic applications. Well-defined spherical AQOAT microcapsules [50.0 ìm ± (24.14)] were formed from 5% AQOAT solution. Cells of Bifidobacterium breve and Lactobacillus plantarum were successfully encapsulated with this technique. However, a 7 log and a 5 log reduction were recorded for B. breve and L. plantarum respectively after 60 min in buffer (P<0.05). Results of this study showed that 1% AQOAT solution lowered viability by 1 log and cell exposure to 10 ml dichloromethane resulted in a 3 log reduction, thus confirming bactericidal properties of both polymer and organic solvent. DCM was however shown to have more bactericidal effects on the cells (P<0.001). Cumulative release trials using 0.2% water blue dye solution showed a 40% loss and encapsulation efficiency (EE) of 73.8% (±14.76). It was recommended that viability counts using this technique be further evaluated using other organic solvents (such as ethyl acetate and chloroform) or other synthetic polymers so as to increase its applicability in the food industry.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Preliminary studies on pharmaceutical microencapsulation for synbiotic application

Evivie

2013

JANS

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“…Little is known about the inhibition of enteropathogens by bifidobacteria of chicken origin. Baffoni et al (2012) observed a reduction of C. jejuni counts after administration of B. longum-based synbiotic. This anti-Campylobacter effect of B. longum is suspected to rely on antibacterial metabolite production and synthesis of unidentified proteinaceous substances.…”

Section: Probiotic Bacteria In Poultry Productionmentioning

confidence: 83%

Chicken intestinal microbiota function with a special emphasis on the role of probiotic bacteria

Cisek¹,

Binek²

2014

Polish Journal of Veterinary Sciences

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Bacterial colonization of the chicken gut by environmental microbes begins immediately after hatching. Composition of the intestinal microbiota is dependent on the surrounding environment, diet variation, pathological conditions, antibiotic therapy, and others. The genomes of all these intestinal microbes form a microbiome which by far outnumbers the host's genome. As a consequence, the microbiome provides additional metabolic functions to the host, including nutrient utilization and absorption, fermentation of non-digestible dietary fiber, synthesis of some vitamins, biotransformation of bile acids, and the well-being of their chicken host. Microorganisms can also directly interact with the lining of the gastrointestinal tract, which may alter the physiology and immunological status of the bird. Since newly hatched broiler chickens demonstrate delayed commensal colonization and low bacterial diversity, the most effective and harmless method available to control the development and composition of the intestinal microbiota is a competitive exclusion treatment by applying probiotic bacteria. Additionally, recent research has shown that probiotic bacteria have a variety of beneficial effects, including counteraction of dysbiosis, promotion of gut health and homeostasis, enhancement of immune defenses and antagonization of infectious agents.

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“…Despite this potential, however, there are few in vivo human or animal studies providing evidence that prebiotics can be used to support specific probiotic strains. Unfortunately, most synbiotic studies, including work in rats (44)(45)(46)(47)(48)(49), mice (50)(51)(52), pigs (53)(54)(55)(56)(57), chickens (58,59), and humans (60), did not employ strainspecific detection methods and therefore did not provide information on the potential synergism between pre-and probiotics. Of the in vivo studies that did discriminate the probiotic strain, most still did not demonstrate that in vivo performance could be enhanced by a prebiotic.…”

Section: Discussionmentioning

confidence: 99%

In Vivo Selection To Identify Bacterial Strains with Enhanced Ecological Performance in Synbiotic Applications

Krumbeck

Maldonado-Gómez

Martínez

et al. 2015

Appl Environ Microbiol

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One strategy for enhancing the establishment of probiotic bacteria in the human intestinal tract is via the parallel administration of a prebiotic, which is referred to as a synbiotic. Here we present a novel method that allows a rational selection of putative probiotic strains to be used in synbiotic applications: in vivo selection (IVS). This method consists of isolating candidate probiotic strains from fecal samples following enrichment with the respective prebiotic. To test the potential of IVS, we isolated bifidobacteria from human subjects who consumed increasing doses of galactooligosaccharides (GOS) for 9 weeks. A retrospective analysis of the fecal microbiota of one subject revealed an 8-fold enrichment in Bifidobacterium adolescentis strain IVS-1 during GOS administration. The functionality of GOS to support the establishment of IVS-1 in the gastrointestinal tract was then evaluated in rats administered the bacterial strain alone, the prebiotic alone, or the synbiotic combination. Strain-specific quantitative real-time PCR showed that the addition of GOS increased B. adolescentis IVS-1 abundance in the distal intestine by nearly 2 logs compared to rats receiving only the probiotic. Illumina 16S rRNA sequencing not only confirmed the increased establishment of IVS-1 in the intestine but also revealed that the strain was able to outcompete the resident Bifidobacterium population when provided with GOS. In conclusion, this study demonstrated that IVS can be used to successfully formulate a synergistic synbiotic that can substantially enhance the establishment and competitiveness of a putative probiotic strain in the gastrointestinal tract.T he mechanistic role of the gastrointestinal (GI) microbiota and its metabolites in maintaining human health has been well demonstrated (1-3). Gut microbes provide several important benefits for their host, including provision of nutrients, development and maturation of the immune system, and protection against pathogens via colonization resistance (4). However, the gut microbiota may also contribute to obesity, inflammatory and autoimmune diseases, and other chronic disease states (5-7). Such diseases are often associated with compositional alterations in the fecal microbiota, a condition referred to as "dysbiosis" (8). Given that the presence of specific types of bacteria and their relative abundance within the gut are considered to affect host health, there is much interest in devising strategies that modulate gut microbiota composition and potentially redress disease-related dysbiotic patterns (9).Dietary approaches currently available to modulate the gut microbiota include prebiotics (10-12), fermentable fibers (13, 14), probiotics (or live biotherapeutics) (15), and synbiotics, which are a combination of a probiotic and a prebiotic (11, 16). According to Kolida and Gibson (16), synbiotics can be either complementary or synergistic. Complementary synbiotics consist of a probiotic and a prebiotic selected to independently confer benefits to the host. In contrast, sy...

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A Bifidobacterium-based synbiotic product to reduce the transmission of C. jejuni along the poultry food chain

Cited by 87 publications

References 34 publications

Preliminary studies on pharmaceutical microencapsulation for synbiotic application

Preliminary studies on pharmaceutical microencapsulation for synbiotic application

Chicken intestinal microbiota function with a special emphasis on the role of probiotic bacteria

In Vivo Selection To Identify Bacterial Strains with Enhanced Ecological Performance in Synbiotic Applications

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