A 5-year multicentre randomized controlled trial comparing personalized, frozen and fresh blastocyst transfer in IVF

Simón, Carlos; Gómez, Carlos José Jaramillo; Cabanillas, Sergio; Vladimirov, Iavor K.; Castillon, G; Gilés, Juan; Boynukalin, K.; Fındıklı, Necati; Bahçeci, Mustafa; Ortega, Israel; Vidal, C.; Funabiki, Miyako; Izquierdo, Alexandra; Lopez, L.; Portela, Susana; Frantz, Nilo; Kulmann, Marcos Iuri Roos; Taguchi, Sagiri; Labarta, Elena; Colucci, F; Mackens, Shari; Santamaría, Xavier Alegre; Muñoz, Elkin; Barrera, Saúl; García-Velasco, Juan A.; Fernández, Mónica; Ferrando, Marcos; Ruíz, M.; Mol, Ben W.; Valbuena, Diana

doi:10.1016/j.rbmo.2020.06.002

Cited by 132 publications

(144 citation statements)

References 22 publications

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“…EF and EB were collected following the previously described procedures (Vilella et al 2013; Simón et al 2020). Briefly, with the patient in the lithotomy position, the vagina and cervix were cleaned.…”

Section: Methodsmentioning

confidence: 99%

Endometrial microbiota composition is associated with reproductive outcome in infertile patients

Moreno

García-Grau

Pérez-Villaroya

et al. 2021

Preprint

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Background: Previous evidence indicates associations between the female reproductive tract microbiome composition and reproductive outcome in infertile patients undergoing assisted reproduction. We aimed to determine whether the endometrial microbiota composition is associated with reproductive outcomes of live birth, biochemical pregnancy, clinical miscarriage, or no pregnancy. Methods: Here we present a multicentre prospective observational study using 16S rRNA gene sequencing to analyse endometrial fluid and biopsy samples before embryo transfer in a cohort of 342 infertile patients asymptomatic for infection undergoing assisted reproductive treatments. Results: A dysbiotic endometrial microbiota profile composed of Atopobium, Bifidobacterium, Chryseobacterium, Gardnerella, Haemophilus, Klebsiella, Neisseria, Staphylococcus and Streptococcus was associated with unsuccessful outcomes. In contrast, Lactobacillus was consistently enriched in patients with live birth outcomes. Conclusions: Our findings indicate that endometrial microbiota composition before embryo transfer is a useful biomarker to predict reproductive outcome, offering an opportunity to further improve diagnosis and treatment strategies.

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Section: Methodsmentioning

confidence: 99%

Endometrial microbiota composition is associated with reproductive outcome in infertile patients

Moreno

García-Grau

Pérez-Villaroya

et al. 2021

Preprint

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“…Unscreened single vs. double blastocyst transfer resulted in equivalent live birth rates irrespective of age in another study [13]. On the other hand, other studies have shown increasing pregnancy rates transferring two as opposed to a single blastocyst [4,14]. The difference between the findings of these studies likely represents the role of endometrial receptivity, transfer technique, and embryo genetics [8,9].…”

mentioning

confidence: 92%

“…Although transferring multiple embryos may impact the pregnancy rate per transfer, multiple pregnancies remain a risk [4,6,14]. We have known that this is the desired outcome for many of our patients, with the opportunity of at one time to complete their family when two children are delivered as twins.…”

mentioning

confidence: 99%

Believing that transferring more embryos will result in increased pregnancy rates: a flawed concept: a SWOT analysis

Dahan¹,

Tannus²

2020

Middle East Fertil Soc J

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A belief exists that transferring multiple embryos can improve pregnancy rates. However, this concept is flawed. Multiple factors affect outcomes when transferring embryos, endometrial receptivity, and rates of aneuploidy among them. In this article, we will discuss how factors can affect outcomes at IVF that are independent of the number of embryos transferred. It is important to understand the role of accessory factors on pregnancy rates to be able to counsel patients as per the number of embryos that should be transferred. An understanding of this concept will also lead to a realistic understanding of how multiple embryo transfers may result in better cumulative pregnancy rates than a single transfer of multiple embryos. Finally, we will present a SWOT analysis diagram to help guide clinical decision-making.

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“…The accuracy and reproducibility was proven to be reliable in subsequent studies [15][16][17]. Several studies have demonstrated that pregnancy outcomes of patients with RIF and infertile couples with conventional IVF [17,18] can be improved by personalized embryo transfer (pET) guided by the ERA test. In addition, relevant results indicate that transcriptomic and proteomic markers provide promising approaches for ER assessment.…”

Section: Introductionmentioning

confidence: 99%

Uterine fluid transcriptome as potential non-invasive biomarker for predicting endometrial receptivity

Zou

et al. 2021

Preprint

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Background: The synchrony between the embryo and the receptive endometrium is essential for successful implantation. Therefore, a reliable non-invasive ER prediction method is highly demanded. We aimed to establish a method that could be used to predict endometrium receptivity non-invasively and to evaluate its clinical application potential in patients undergoing IVF. Methods: The non-invasive RNA-seq based endometrial receptivity test (nirsERT) was established by sequencing and analyzing the RNA of uterine fluid from 48 IVF patients with normal ER. Subsequently, 22 IVF patients were recruited and analyzed the correlation between the predicted results of nirsERT and pregnancy outcomes. Results: 87 marker genes and 3 hub genes were selected to establish the nirsERT. 10-fold cross-validation resulted in a mean accuracy of 93.0%. A small cohort retrospective observation showed that 77.8% (14/18) of IVF patients predicted with normal WOI had successful intrauterine pregnancies, while none of the 3 patients with displaced WOI had successful pregnancy. Conclusions: nirsERT is potential for a non-invasive, accurate and same cycle testing for ER in reproductive clinic.

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A 5-year multicentre randomized controlled trial comparing personalized, frozen and fresh blastocyst transfer in IVF

Abstract: Endometrial receptivityEndometrial receptivity analysis (ERA) Fresh embryo transfer (ET) Frozen embryo transfer (FET) Personalized embryo transfer (PET) Window of implantation (WOI)

Cited by 132 publications

References 22 publications

Endometrial microbiota composition is associated with reproductive outcome in infertile patients

Endometrial microbiota composition is associated with reproductive outcome in infertile patients

Believing that transferring more embryos will result in increased pregnancy rates: a flawed concept: a SWOT analysis

Uterine fluid transcriptome as potential non-invasive biomarker for predicting endometrial receptivity

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