A 3D tumor microenvironment regulates cell proliferation, peritoneal growth and expression patterns

Loessner, Daniela; Rockstroh, Anja; Shokoohmand, Ali; Holzapfel, Boris Michael; Wagner, Ferdinand; Baldwin, Jeremy; Boxberg, Melanie; Schmalfeldt, Barbara; Lengyel, Ernst; Clements, Judith A.; Hutmacher, Dietmar W.

doi:10.1016/j.biomaterials.2018.10.014

Cited by 43 publications

(35 citation statements)

References 52 publications

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“…They used "3D co-cultured" hydrogel-encapsulated ovarian cancer cells with mesothelial cell-layered melt electrospun written scaffolds. These ex vivo tumor implants engrafted well and mimicked the same progression, invasion, and microenvironment as peritoneal implants of natural origin in mice [87].…”

Section: D-biomimetics Peritoneal Implantsmentioning

confidence: 86%

“…Several studies have used these scaffolds to make an ex vivo tumor recapitulating the complexity of the tumor microenvironment. This has been achieved by including cancer cells, immune cells, fibroblasts, growth factors, and stromal components [86][87][88]. In PCa, this methodology has been used for the development of high-complexity bona fide peritoneal implants, although it is debatable whether this should be defined as an in vivo model per se.…”

Section: D-biomimetics Peritoneal Implantsmentioning

confidence: 99%

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Mouse Models of Peritoneal Carcinomatosis to Develop Clinical Applications

Bella

Trani

Fernandez‐Sendin

et al. 2021

Cancers

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Peritoneal carcinomatosis of primary tumors originating in gastrointestinal (e.g., colorectal cancer, gastric cancer) or gynecologic (e.g., ovarian cancer) malignancies is a widespread type of tumor dissemination in the peritoneal cavity for which few therapeutic options are available. Therefore, reliable preclinical models are crucial for research and development of efficacious treatments for this condition. To date, a number of animal models have attempted to reproduce as accurately as possible the complexity of the tumor microenvironment of human peritoneal carcinomatosis. These include: Syngeneic tumor cell lines, human xenografts, patient-derived xenografts, genetically induced tumors, and 3D scaffold biomimetics. Each experimental model has its own strengths and limitations, all of which can influence the subsequent translational results concerning anticancer and immunomodulatory drugs under exploration. This review highlights the current status of peritoneal carcinomatosis mouse models for preclinical development of anticancer drugs or immunotherapeutic agents.

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Section: D-biomimetics Peritoneal Implantsmentioning

confidence: 86%

Section: D-biomimetics Peritoneal Implantsmentioning

confidence: 99%

Mouse Models of Peritoneal Carcinomatosis to Develop Clinical Applications

Bella

Trani

Fernandez‐Sendin

et al. 2021

Cancers

View full text Add to dashboard Cite

show abstract

“…The incorporation of a hydrogel within geometrically varied micro-fibers produced by MEW has fulfilled the required mechanical properties to mimic the function of fibrous ECM of soft tissues [84,[109][110][111][112][113][114][115][116]. Mainly, the construction procedure of hybrid hydrogel-MEW composites has two steps, which are the fabrication of fibers via MEW and infiltration of hydrogel in manufactured fibers [84,[109][110][111][112][113][114][115][116][117].…”

Section: Reinforcement Mechanism In Melt Electrowritten Fiber-hydrogementioning

confidence: 99%

“…The MEW-hydrogel hybrid manufacturing approach has also allowed for the development of 3D in vitro tissue models, such as neural and tumor cultures [117,120]. For example, Glycine receptor transfected (GTR; ligand-gated ion channel) Ltk-11 cells encapsulated in Matrigel reinforced with melt electrowritten PCL scaffold.…”

Section: Biological and Mechanical Aspects Of Reinforced Composites Imentioning

confidence: 99%

“…In another study, patient-derived ovarian cancer cells were encapsulated in PEG hydrogel infiltrated into PCL fiber scaffold. It was used to mimic the tissue environment for the investigation of malignant behavior and tumor-promoting signals [117]. Designed models showed clinically similar gene expression of ovarian cancer with the samples that were obtained from a high-grade serous ovarian cancer patient.…”

Section: Biological and Mechanical Aspects Of Reinforced Composites Imentioning

confidence: 99%

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Biomimicry in Bio-Manufacturing: Developments in Melt Electrospinning Writing Technology Towards Hybrid Biomanufacturing

et al. 2019

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Melt electrospinning writing has been emerged as a promising technique in the field of tissue engineering, with the capability of fabricating controllable and highly ordered complex three-dimensional geometries from a wide range of polymers. This three-dimensional (3D) printing method can be used to fabricate scaffolds biomimicking extracellular matrix of replaced tissue with the required mechanical properties. However, controlled and homogeneous cell attachment on melt electrospun fibers is a challenge. The combination of melt electrospinning writing with other tissue engineering approaches, called hybrid biomanufacturing, has introduced new perspectives and increased its potential applications in tissue engineering. In this review, principles and key parameters, challenges, and opportunities of melt electrospinning writing, and particularly, recent approaches and materials in this field are introduced. Subsequently, hybrid biomanufacturing strategies are presented for improved biological and mechanical properties of the manufactured porous structures. An overview of the possible hybrid setups and applications, future perspective of hybrid processes, guidelines, and opportunities in different areas of tissue/organ engineering are also highlighted.

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The molecular function of kallikrein‐related peptidase 14 demonstrates a key modulatory role in advanced prostate cancer

et al. 2019

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Kallikrein-related peptidase 14 (KLK14) is one of the several secreted KLK serine proteases involved in prostate cancer (PCa) pathogenesis. While relatively understudied, recent reports have identified KLK14 as overexpressed during PCa development. However, the modulation of KLK14 expression during PCa progression and the molecular and biological functions of this protease in the prostate tumor microenvironment remain unknown. To determine the modulation of KLK14 expression during PCa progression, we analyzed the expression levels of KLK14 in patient samples using publicly available databases and immunohistochemistry. In order to delineate the molecular mechanisms involving KLK14 in PCa progression, we integrated proteomic, transcriptomic, and in vitro assays with the goal to identify substrates, related-signaling pathways, and functional roles of this protease. We showed that KLK14 expression is elevated in advanced PCa, and particularly in metastasis. Additionally, KLK14 levels were found to be decreased in PCa tissues from patients responsive to neoadjuvant therapy compared to untreated patients. Furthermore, we also identified that KLK14 expression reoccurred in patients who developed castrate-resistant PCa. The combination of proteomic and transcriptomic analysis as well as functional assays revealed several new KLK14 substrates (agrin, desmoglein 2, vitronectin, laminins) and KLK14regulated genes (Interleukin 32, midkine, SRY-Box 9), particularly an

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A 3D tumor microenvironment regulates cell proliferation, peritoneal growth and expression patterns

Cited by 43 publications

References 52 publications

Mouse Models of Peritoneal Carcinomatosis to Develop Clinical Applications

Mouse Models of Peritoneal Carcinomatosis to Develop Clinical Applications

Biomimicry in Bio-Manufacturing: Developments in Melt Electrospinning Writing Technology Towards Hybrid Biomanufacturing

The molecular function of kallikrein‐related peptidase 14 demonstrates a key modulatory role in advanced prostate cancer

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