A 31-bp indel in the 5′ UTR region of GNB1L is significantly associated with chicken body weight and carcass traits

Ren, Tuanhui; Yang, Ying; Lin, Wujian; Li, Wangyu; Xian, Mingjian; Fu, Rong; Zhang, Zihao; Mo, Guodong; Luo, Wen; Zhang, Xiquan

doi:10.1186/s12863-020-00900-z

Cited by 6 publications

(2 citation statements)

References 31 publications

(36 reference statements)

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“…As a new generation of molecular genetic markers, indel plays an important role in animal genetics and breeding research ( Wang et al, 2013 ; Zang et al, 2016 ; Li et al, 2017 ). Previous studies have been applied to poultry genetics and breeding by mining effective indels of different muscle growth and development-related genes ( Ren et al, 2020 ). We found an interesting indel of 42 bp by screening the genetic sequences of the chicken RPL3L gene in different breeds, and its DD genotype has a significant mutation rate in large chicken breeds.…”

Section: Discussionmentioning

confidence: 99%

Mining of chicken muscle growth genes and the function of important candidate gene RPL3L in muscle development

et al. 2022

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The birth weight of chickens does not significantly affect the weight at slaughter, while the different growth rate after birth was one of the important reasons for the difference in slaughter weight. Also, the increase in chickens’ postnatal skeletal muscle weight is the main cause of the slaughter weight gain, but which genes are involved in this biological process is still unclear. In this study, by integrating four transcriptome datasets containing chicken muscles at different developmental times or different chicken tissues in public databases, a total of nine candidate genes that may be related to postnatal muscle development in chickens were obtained, including RPL3L, FBP2, ASB4, ASB15, CKMT2, PGAM1, YIPF7, PFKM, and LDHA. One of these candidate genes is RPL3L, whose 42 bp insertion/deletion (indel) mutation significantly correlated with multiple carcass traits in the F2 resource population from Xinghua chickens crossing with White Recessive Rock (WRR) chickens, including live weight, carcass weight, half eviscerated weight, eviscerated weight, breast meat weight, wing weight, leg muscle shear force, and breast muscle shear force. Also, there was a very significant difference between different genotypes of the RPL3L 42 bp indel mutation in these trains. Further experiments showed that RPL3L was highly expressed in chicken skeletal muscle, and its overexpression could promote the proliferation and inhibit the differentiation of chicken myoblasts by regulating ASB4 and ASB15 expression. Our findings demonstrated that the RPL3L 42 bp indel may be one of the molecular markers of chicken weight-related traits.

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Section: Discussionmentioning

confidence: 99%

Mining of chicken muscle growth genes and the function of important candidate gene RPL3L in muscle development

et al. 2022

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“…In addition, chicken growth and carcass performance are the main breeding indicators [ 23 ]. The growth rate and weight of native Chinese chickens are relatively low compared with those of commercial broilers [ 24 ]. Recent studies have found that, in humans, the interacting protein NUDT3 of QPCTL is an important genetic biomarker of sarcopenia in elderly individuals.…”

Section: Discussionmentioning

confidence: 99%

QPCTL Affects the Daily Weight Gain of the F2 Population and Regulates Myogenic Cell Proliferation and Differentiation in Chickens

Ren

Lin

Yang

et al. 2022

Animals

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Molecular breeding can accelerate the process of animal breeding and improve the breeding efficiency. To date, many Indel molecular markers have been identified in livestock and poultry, but how Indels affect economic traits is not well understood. For molecular breeding, it is crucial to reveal the mechanism of action of Indels and to provide more accurate information. The purpose of this study was to investigate how the 52/224-bp multiallelic Indels of the chicken QPCTL promoter area affect the daily weight gain of chickens and the potential regulatory mechanism of the QPCTL gene. The analysis was conducted by association analysis, qPCR, dual-fluorescence assay and Western blotting. The results showed that Indels in the QPCTL promoter region were significantly associated with the daily weight gain in chickens and that QPCTL expression showed a decreasing trend in embryonic breast muscle tissues. Furthermore, QPCTL expression was significantly higher in breast muscle tissues of the AC genotype than in those of the AB and BB genotypes. Based on the transcriptional activity results, the pGL3-C vector produced more luciferase activity than pGL3-A and pGL3-B. In addition, overexpression of QPCTL promoted chicken primary myoblast (CPM) proliferation and inhibited differentiation. The results of this study suggest that Indels in the promoter region of the QPCTL gene may regulate the proliferation and differentiation of CPMs by affecting the expression of QPCTL, which ultimately affects the growth rate of chickens. These Indels have important value for the molecular breeding of chickens, and QPCTL can be used as a candidate gene to regulate and improve chicken growth and development.

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Hundreds of independent midsize deletions mediate DNA loss in wild relatives of Red Jungle Fowl

2023

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A 31-bp indel in the 5′ UTR region of GNB1L is significantly associated with chicken body weight and carcass traits

Cited by 6 publications

References 31 publications

Mining of chicken muscle growth genes and the function of important candidate gene RPL3L in muscle development

Mining of chicken muscle growth genes and the function of important candidate gene RPL3L in muscle development

QPCTL Affects the Daily Weight Gain of the F2 Population and Regulates Myogenic Cell Proliferation and Differentiation in Chickens

Hundreds of independent midsize deletions mediate DNA loss in wild relatives of Red Jungle Fowl

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