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Sathananthan, A. Henry

doi:10.1023/a:1023056804548

Cited by 56 publications

(6 citation statements)

References 27 publications

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“…The evidence that elongated radial sperm aster was involved in the movement and apposition of male and female pronuclei of buffalo's zygote suggested that the centrosomal material is primarily paternally inherited and is similar to previous reports in other mammalian species such as human [1, 2], sheep [3], rabbit [4], porcine [5], bovine [6–8], and rhesus monkey [9]. However, this is different in mouse because cytoplasmic microtubules in the cytoplasm originate from maternal centrosomes and sperm astral microtubules were not detected in the decondensing paternal chromatin [10, 41].…”

Section: Discussionsupporting

confidence: 85%

“…The microtubules actively involve in the process of fertilization by the formation of microtubule networks that facilitate the migration and apposition of male and female pronuclei. These microtubules are paternally inherited in most mammalian species, including human [ 1 , 2 ], sheep [ 3 ], rabbit [ 4 ], porcine [ 5 ], bovine [ 6 – 8 ], and rhesus monkey [ 9 ]. On the other hand, the paternal centrosome in the ooplasm is functionally absent in mice, and thus the syngamy of the two pronuclei requires the maternal centrosome [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

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Chronological Reorganization of Microtubules, Actin Microfilaments, and Chromatin during the First Cell Cycle in Swamp Buffalo (Bubalus bubalis) Embryos

Chankitisakul

Tharasanit

Tasripoo

et al. 2010

Veterinary Medicine International

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This paper aimed to study the dynamics of early embryonic development, in terms of redistribution of cytoskeleton (microtubules, actin microfilaments) and chromatin configurations during the first cell cycle in swamp buffalo embryos. Oocytes were matured and fertilized in vitro, and they were fixed at various time points after IVF. At 6 h after IVF, 44.4% matured oocytes were penetrated by spermatozoa. Partial ZP digestion, however, did not improve fertilization rate compared to control (P > .05). At 12 h after IVF, the fertilized oocytes progressed to the second meiotic division and formed the female pronucleus simultaneously with the paternal chromatin continued to decondense. A sperm aster was observed radiating from the base of the decondensing sperm head. At 18 h after IVF, most presumptive zygotes had reached the pronuclear stage. The sperm aster was concurrently enlarged to assist the migration and apposition of pronuclei. Cell cleavage was facilitated by microfilaments and firstly observed by 30 h after IVF. In conclusion, the cytoskeleton actively involves with the process of fertilization and cleavage in swamp buffalo oocytes. The centrosomal material is paternally inherited. Fertilization failure is predominantly caused by poor sperm penetration. However, partial digestion of ZP did not improve fertilization rate.

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Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Chronological Reorganization of Microtubules, Actin Microfilaments, and Chromatin during the First Cell Cycle in Swamp Buffalo (Bubalus bubalis) Embryos

Chankitisakul

Tharasanit

Tasripoo

et al. 2010

Veterinary Medicine International

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“…The sperm centriole duplicates during the pronuclear stage and separates after syngamy to serve as the mitotic center from the first cleavage division up to the blastocyst stage [27, 28]. During that period the maternal centrosome is not functional [29]. …”

Section: Discussionmentioning

confidence: 99%

Direct Unequal Cleavages: Embryo Developmental Competence, Genetic Constitution and Clinical Outcome

et al. 2016

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ObjectiveTo investigate the prevalence, developmental potential, chromosomal constitution and clinical outcome of embryos with direct unequal cleavages (DUC).DesignA retrospective observational study.SettingAcademic Institution.Participant21,261 embryos from 3,155 cycles cultured in EmbryoScope®.ResultsThe total incidence of DUCs per embryo occupying the first three cleavages were 26.1%. Depending of the cell stage, DUC rate was 9.8% at first cleavage (DUC-1), 9.1% at second cleavage (DUC-2), and 3.7% at third cleavage (DUC-3) with 3.6% of embryos exhibiting multiple DUCs (DUC-Plus). The occurrence of DUCs was not correlated with female gamete age or source. The incidence of DUC-1 was significantly higher in embryos fertilized by epididymal and testicular sperm (13.6% and 11.4%, respectively) compared to ejaculated sperm (9.1%, all p<0.05). The total incidences of DUCs were strongly correlated with the onset of blastomere multinucleation (MNB) during the first three divisions. In MNB embryos, DUCs incidence are two to three times more likely to develop when compared to non-MNB embryos (OR = 3.11, 95% CI [2.64, 3.67] at 1-cell stage, OR = 2.64, 95% CI [2.39, 2.91] at 2-cell stage and OR = 2.51, 95% CI [1.84, 3.43] at 4-cell stage). The blastocyst formation rates gradually decreased from 61.0% in non-DUC to 40.2% in DUC-3, 18.8% in DUC-2, 8.2% in DUC-1 and 5.6% in multiple DUC embryos (DUC-Plus). The known implantation rates (FH) for day 3 (D3) transfers were 12.42% (n = 3172) in Non-DUC embryos, 6.3% (n = 127) in DUC-3, and 2.7% (n = 260) in DUC-2 embryos. No live births resulted from either DUC-1 (n = 225) or DUC-Plus (n = 100) embryo transfers. For blastocyst transfers, lower implantation rates (33.3%) but similar live birth (LB) rates (40%) were observed if DUC blastocysts were transferred. Comparatively rates in Non-DUC blastocyst were 45.2% and 34.8%, respectively. The euploid rate gradually increased from DUC-1, -2, -3 to Non-DUC (13.3%, 19.5%, 33.3%, 45.6%, p<0.001) for D3 biopsied embryos. Interestingly, the trend of decreased euploidy disappeared in DUC D5/6 biopsied embryos and similar rates were exemplified in DUC (D5 56.3%, D6 35.6%) vs. non-DUC (D5 51.4%, D6 33.8%) embryos.ConclusionBlastocyst formation, implantation potential and euploid rate were significantly reduced in DUC embryos. DUC embryos should be deselected for D3 transfers, but should be culture to blastocyst stage for possible ET.

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“…This may be related to incomplete DNA replication and the consequently high frequency of chromosomal abnormalities, as observed in human and bovine embryos [11,18]. The cause of direct cleavage is the formation of tripolar or tetrapolar spindles, which is associated with the abnormal distribution of chromosomes in the blastomeres [18,19]. The most frequently occurring abnormality observed in human IVF embryos is the fragmentation of the cytoplasm [4,8].…”

Section: Discussionmentioning

confidence: 99%

Using Time Lapse Monitoring for Determination of Morphological Defect Frequency in Feline Embryos after in Vitro Fertilization (IVF)

Kij

Kochan

Nowak

et al. 2019

Animals

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Some human, bovine, and mouse in vitro fertilized (IVF) embryos with morphokinetic abnormalities such as fragmentation, direct cleavage, and cytoplasmic vacuoles have the potential to reach the blastocyst stage, which is related to a high potential for implantation. The latest techniques of embryo development observation to enable the evaluation and selection of embryos are based on time lapse monitoring (TLM). The aim of this study was to determine the frequency of morphological defects in feline embryos, their competence to reach the blastocyst stage, and their ability to hatch. Oocyte-cumulus complexes were isolated after the scarification of ovaries and matured in vitro. Matured oocytes were fertilized in vitro by capacitated spermatozoa. Randomly selected oocytes were observed by TLM for seven-to-eight days. Out of 76 developed embryos, 41 were morphologically normal, of which 15 reached the blastocyst stage. Of 35 abnormally developed embryos, 17 reached the blastocyst stage, of which six had single aberrations and 11 had multiple aberrations. The hatching rate (%) was 15.6% in normally cleaving embryos, 6.25% in embryos with single aberrations, and 3.33% in those with multiple aberrations. The present study reports the first results, found by using TLM, about the frequency of the morphological defects of feline embryos, their competence to reach the blastocyst stage, and their ability to hatch.

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Abstract: The human embryo shows paternal centrosome inheritance and perpetuation like most other animals. Inheritance of defective centrosomes may lead to abnormal cleavage and contribute to infertility.

Cited by 56 publications

References 27 publications

Chronological Reorganization of Microtubules, Actin Microfilaments, and Chromatin during the First Cell Cycle in Swamp Buffalo (Bubalus bubalis) Embryos

Chronological Reorganization of Microtubules, Actin Microfilaments, and Chromatin during the First Cell Cycle in Swamp Buffalo (Bubalus bubalis) Embryos

Direct Unequal Cleavages: Embryo Developmental Competence, Genetic Constitution and Clinical Outcome

Using Time Lapse Monitoring for Determination of Morphological Defect Frequency in Feline Embryos after in Vitro Fertilization (IVF)

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