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Araújo, S. M. S. R.; Silva, C. C.; Pompolo, S. G.; Perfectti, Francisco; Camacho, Juan Pedro M.

doi:10.1023/a:1020970820513

Cited by 12 publications

(3 citation statements)

References 24 publications

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“…Heteromorphism in size of a particular chromosome pair was found among all metaphases analyzed for homologous chromosomes by mapping 18S rDNA sites. Apparently this is a recurrent characteristic in Meliponini (Rocha et al 2007;Menezes et al 2014;Andrade-Souza et al 2018), and it is due to the repetitive nature of ribosomal DNAs, that results in errors during duplication of the genetic material or uneven crossing-over followed by deletion of a small part of the chromosome (Araújo et al 2002;Mampumbu 2002).…”

Section: Discussionmentioning

confidence: 99%

Chromosomal mapping of repetitive DNA in Melipona seminigra merrillae Cockerell, 1919 (Hymenoptera, Apidae, Meliponini)

Barbosa¹,

Schneider²,

Goll³

et al. 2021

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Melipona Illiger, 1806 is represented by 74 known species of stingless bees, distributed throughout the Neotropical region. Cytogenetically it is the most studied stingless bee genus of the tribe Meliponini. Member species are divided in two groups based on the volume of heterochromatin. This study aim was to analyze the composition and organization of chromatin of the stingless bee subspecies Melipona seminigra merrillae Cockerell, 1919 using classical and molecular cytogenetic techniques, so contributing to a better understanding of the processes of chromosomal changes within the genus. We confirm that M. seminigra merrillae has a chromosome number of 2n = 22 and n = 11, results that differ from those reported for the genus in the absence of B chromosomes. The heterochromatic pattern revealed a karyotype composed of chromosomes with a high heterochromatin content, which makes it difficult to visualize the centromere. Silver nitrate impregnation (Ag-NOR) showed transcriptionally active sites on the second chromosomal pair. Staining of base-specific fluorophores DAPI-CMA3 indicated a homogeneous distribution of intensely DAPI-stained heterochromatin, while CMA3 markings appeared on those terminal portions of the chromosomes corresponding to euchromatin. Similar to Ag-NOR, fluorescence in situ hybridization (FISH) with 18S ribosomal DNA probe revealed distinct signals on the second pair of chromosomes. Microsatellite mapping (GA)15 showed markings distributed in euchromatic regions, while mapping with (CA)15 showed marking patterns in heterochromatic regions, together with a fully marked chromosome pair. Microsatellite hybridization, both in heterochromatic and euchromatic regions, may be related to the activity of transposable elements. These are capable of forming new microsatellites that can be dispersed and amplified in different regions of the genome, demonstrating that repetitive sequences can evolve rapidly, thus resulting in within-genus diversification.

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Section: Discussionmentioning

confidence: 99%

Chromosomal mapping of repetitive DNA in Melipona seminigra merrillae Cockerell, 1919 (Hymenoptera, Apidae, Meliponini)

Barbosa¹,

Schneider²,

Goll³

et al. 2021

CCG

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“…The correlation between NORs and CMA 3 bands is quite common in some insects, including beetles ( Camacho et al 1991 ; Vitturi et al 1999 ; Colomba et al 2000 ; Maffei et al 2001 ; Kuznetsova et al 2001 ; Araújo et al 2002 ; Brito et al 2003 ; Nechayeva et al 2004 ). Silver staining mainly reveals transcriptionally active NORs ( Sumner 1990 ), as opposed to fluorochrome CMA 3 staining which labels NORs independently of their activity ( Schmid and Guttenbach 1988 ).…”

Section: Discussionmentioning

confidence: 99%

Karyotype analysis of four jewel-beetle species (Coleoptera, Buprestidae) detected by standard staining, C-banding, AgNOR-banding and CMA3/DAPI staining

Karagyan¹,

Lachowska²,

Kalashian³

2012

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The male karyotypes of Acmaeodera pilosellae persica Mannerheim, 1837 with 2n=20 (18+neoXY), Sphenoptera scovitzii Faldermann, 1835 (2n=38-46), Dicerca aenea validiuscula Semenov, 1895 -2n=20 (18+Xy p ) and Sphaerobothris aghababiani Volkovitsh et Kalashian, 1998 -2n=16 (14+Xy p ) were studied using conventional staining and different chromosome banding techniques: C-banding, AgNOR-banding, as well as fluorochrome Chromomycin A 3 (CMA 3 ) and DAPI. It is shown that C-positive segments are weakly visible in all four species which indicates a small amount of constitutive heterochromatin (CH). There were no signals after DAPI staining and some positive signals were discovered using CMA 3 staining demonstrating absence of AT-rich DNA and presence of GC-rich clusters of CH. Nucleolus organizing regions (NORs) were revealed using Ag-NOR technique; argentophilic material mostly coincides with positive signals obtained using CMA 3 staining.

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“…The absence of a CMA3+/DAPI- block in Trypoxylon lactitarse and Trypoxylon rugifrons in euchromatic regions may be attributed to differences in the molecular composition of the rDNA. Using fluorescent in situ hybridization with an rDNA probe in Trypoxylon albitarse , Araújo et al (2002) found that all rRNA genes are located on the heterochromatic arm in a single chromosome pair.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of constitutive heterochromatin in three species of Trypoxylon (Hymenoptera, Crabronidae, Trypoxylini) by CMA3/DAPI staining

Menezes¹,

Carvalho²,

Silva³

et al. 2011

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Previous cytogenetic analyses in Trypoxylon Latreille, 1796 have been basically restricted to C-banding. In the present study, base-specific CMA3 and DAPI fluorochrome staining were used to characterize the constitutive heterochromatin in three Trypoxylon species. The heterochromatin was GC-rich in all the species studied; however, in Trypoxylon nitidum F. Smith, 1856the molecular composition of the heterochromatinwasdifferent among chromosome pairs. Conversely, the euchromatin was AT-rich in the three species. These results suggest high conservatism in the euchromatic regions as opposed to the heterochromatic regions that have a high rate of changes. In this study, we report the karyotype of Trypoxylon rugifrons F. Smith, 1873which has the lowest chromosome number in the genus and other characteristics of the likely ancestral Trypoxylon karyotype.

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Cited by 12 publications

References 24 publications

Chromosomal mapping of repetitive DNA in Melipona seminigra merrillae Cockerell, 1919 (Hymenoptera, Apidae, Meliponini)

Chromosomal mapping of repetitive DNA in Melipona seminigra merrillae Cockerell, 1919 (Hymenoptera, Apidae, Meliponini)

Karyotype analysis of four jewel-beetle species (Coleoptera, Buprestidae) detected by standard staining, C-banding, AgNOR-banding and CMA3/DAPI staining

Molecular characterization of constitutive heterochromatin in three species of Trypoxylon (Hymenoptera, Crabronidae, Trypoxylini) by CMA3/DAPI staining

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