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Azeem, Amir; Neelagund, YF; Rathod, Vandana

doi:10.1023/a:1008011603096

Cited by 12 publications

(1 citation statement)

References 2 publications

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“…It has long been a subject of both research and industrial interest for many years to produce microbial oils through oleaginous microorganisms that involve bacteria, yeasts, moulds and algae. Microbial polyunsaturated fatty acids, such as docosahexaenoic acid (DHA) and arachidonic acid (ARA), are very important in nutrition (Azeem et al 1999;Ratledge 2004). Because of their similar composition of fatty acids to that of vegetable oils, microbial oils are now the potential feedstock for biodiesel production (Li et al 2007;Zhu et al 2008).…”

Section: Introductionmentioning

confidence: 99%

A quick isolation method for mutants with high lipid yield in oleaginous yeast

Wang

Dong

et al. 2009

World J Microbiol Biotechnol

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A novel method has been developed to easily isolate the mutants with high lipid yield after irradiating oleaginous yeast cells with carbon ions of energy of 80 MeV/u. Pre-selection of the mutants after ion irradiation was performed with culture medium in which the concentration of cerulenin, a potent inhibitor of fatty acid synthetase, was at 8.96 lmol/l. Afterwards, lipid concentration in the fermentation broth of the pre-selected colonies was estimated by the sulfo-phospho-vanillin reaction instead of the conventional methanol-chloroform extraction. Two mutants with high lipid yield have been successfully selected out by the combined method. This easy and simple method is much less time-consuming but very efficient in the mutant isolation, and it has demonstrated great potential on mutation breeding in oleaginous microorganism.

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