Each well of an Immulon 4HBX 96-well flat-bottomed microtiter plate (Dynax Technologies Inc.) was coated with 50 µl of 8 µg/ml goat anti-human IgG (Fcspecific; Sigma-Aldrich Corp.) in 0.05 M carbonate-bicarbonate buffer (pH 9.6; Sigma-Aldrich Corp.). Plates were washed with PBS containing 0.25 % bovine serum albumin (PBA), after which 2-fold dilution series of the purified siglec-Fc chimera were added to the wells and incubated for 2 h at ambient temperature. Unbound siglec-Fc protein was removed by washing w…
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