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Lipase-Catalyzed Synthesis of 6-O-D-Glucosyldecanoate inTert-Butanol: Reaction Optimization and Effect of Mixing Power Input

“…Since there is only one peak was observed at 2.3042 ppm, this is a strong indication that only glucose monoester was produced in the reaction. this is also in agreement with the reported specificity of the lipase used in this study which favors the formation of monoester (8). On the other hand, if diester was present, there would be two separate peaks with the peaks located closer to 3 ppm.…”

Regiospecific alkaline protease-catalyzed divinyl acyl transesterifications of primary hydroxyl groups of mono- and di-saccharides in pyridine

“…21 Most sugar esters reported have been anomeric mixtures. 4,5,14 Degn et al 22 synthesized some long-chain fatty acid esters of glucose having a high proportion of the a anomer in tert-butyl alcohol as solvent, using immobilized lipases from C. antarctica and M. miehei. However, only when the chain length of acyl donors increased to a certain number (>8), did the enzyme show selective preference for the a-anomer substrate, and no pure a-anomeric products were obtained.…”

Development of a microtiter plate-based assay for the detection of lipase-catalyzed transesterifications in organic solvents

“…Since the late 1980s lipases have gained special interest due to their reverse hydrolytic and transesterification activity exhibited when employed in anhydrous reaction media (Klibanov 1989). Thus, lipases have been widely used to synthesize ester bonds; for example for the enzymatic synthesis of glycolipids, where different lipases in varying formulations have been used in several organic solvents (Coulon et al 1996; Degn et al 1999). When performing enzymatic glycolipid syntheses, the choice of solvent and catalyst are crucial parameters.…”