Untitled

Honys, David; Oh, Sung‐Aeong; Reňák, David; Donders, Maarten; Šolcová, Blanka; Johnson, James; Boudova, R; Twell, David

doi:10.1186/1471-2229-6-31

Cited by 44 publications

(23 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Both VvWRKY13 , 59 and 57 , belonging to Group I, were higly expressed in stamen and pollen tissues and are closely related to Arabidopsis AtWRKY34 , which was shown to have a role in coordinating gene expression during the formation of the tapetum and microspores. 82 …”

Section: Discussionmentioning

confidence: 99%

Genome and transcriptome analysis of the grapevine (Vitis vinifera L.) WRKY gene family

et al. 2014

View full text Add to dashboard Cite

The plant WRKY gene family represents an ancient and complex class of zinc-finger transcription factors (TFs) that are involved in the regulation of various physiological processes, such as development and senescence, and in plant response to many biotic and abiotic stresses. Despite the growing number of studies on the genomic organisation of WRKY gene family in different species, little information is available about this family in grapevine (Vitis vinifera L.). In the present study, a total number of 59 putative grapevine WRKY transcription factors (VvWRKYs) were identified based on the analysis of various genomic and proteomic grapevine databases. According to their structural and phylogentic features, the identified grapevine WRKY transcription factors were classified into three main groups. In order to shed light into their regulatory roles in growth and development as well as in response to biotic and abiotic stress in grapevine, the VvWRKYs expression profiles were examined in publicly available microarray data. Bioinformatics analysis of these data revealed distinct temporal and spatial expression patterns of VvWRKYs in various tissues, organs and developmental stages, as well as in response to biotic and abiotic stresses. To also extend our analysis to situations not covered by the arrays and to validate our results, the expression profiles of selected VvWRKYs in response to drought stress, Erysiphe necator (powdery mildew) infection, and hormone treatments (salicilic acid and ethylene), were investigated by quantitative real-time reverse transcription PCR (qRT-PCR). The present study provides a foundation for further comparative genomics and functional studies of this important class of transcriptional regulators in grapevine.

show abstract

Section: Discussionmentioning

confidence: 99%

Genome and transcriptome analysis of the grapevine (Vitis vinifera L.) WRKY gene family

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Like pAt5g17340:UidA:GFP, there are other marker lines that use Arabidopsis promoters to induce convincing GUS staining in all the male gametophytic stages (Honys et al 2006), yet the reported staining intensity in their uninucleate microspores is not as strong as in the pAt5g17340:UidA:GFP marker line.…”

Section: Discussionmentioning

confidence: 99%

“…Several Arabidopsis promoter sequences have also been shown to induce strong GUS staining in Arabidopsis flowers, in a male gametophyte and anther specific manner. However, these Arabidopsis promoters, either do not induce GUS staining in all stages of the male gametophyte, or if they do, they do not exhibit strong GUS staining at some of these stages (in particular in uninucleate microspores) (Moore et al 1997; Li et al 1998; Honys et al 2006; Takeda et al 2006; Gibalová et al 2009; Phan et al 2011). …”

Section: Introductionmentioning

confidence: 99%

A novel Arabidopsis marker line that strongly labels uninucleate microspores and the subsequent male gametophyte development stages

Costa-Nunes¹

2013

SpringerPlus

View full text Add to dashboard Cite

Here is reported a novel histological marker line that strongly and specifically labels the male gametophyte in Arabidopsis flowers, from the uninucleate microspore to the pollination stage. In this marker line, the expression of the UidA and GFP marker genes is driven by the promoter of the At5g17340 Arabidopsis gene, a gene highly expressed in the male gametophyte. Strong GUS histochemical staining is detected in the uninucleate microspores. Strong GUS, and GFP, histological labelling is also detected in the subsequent stages of male gametophyte development. The potential applications of this marker line are discussed.Electronic supplementary materialThe online version of this article (doi:10.1186/2193-1801-2-237) contains supplementary material, which is available to authorized users.

show abstract

“…The BP clones pDONR-P4-P1R- pLAT52 and pDONR-P2R-P3-mRFP were kindly provided by Prof. David Twell (Leicester University, UK) (Eady et al, 1994). MSP1 promoter was cloned into pDONR-P4-P1R as described previously (Honys et al, 2006). WT plants were transformed via floral dip (Clough & Bent, 1998), and T2 or T3 plants homozygous for the transgene were used in this study.…”

Section: Methodsmentioning

confidence: 99%

Natural depletion of H1 in sex cells causes DNA demethylation, heterochromatin decondensation and transposon activation

Vickers

Zhang

et al. 2018

Preprint

View full text Add to dashboard Cite

10Transposable elements (TEs), the movement of which can damage the genome, are 11 epigenetically silenced in eukaryotes. Intriguingly, TEs are activated in the sperm companion 12 cell -vegetative cell (VC) -of the flowering plant Arabidopsis thaliana. However, the extent 13 and mechanism of this activation are unknown. Here we show that about 100 heterochromatic 14 TEs are activated in VCs, mostly by DEMETER-catalyzed DNA demethylation. We further 15 demonstrate that DEMETER access to some of these TEs is permitted by the natural depletion 16 of linker histone H1 in VCs. Ectopically expressed H1 suppresses TEs in VCs by reducing 17 DNA demethylation and via a methylation-independent mechanism. We demonstrate that H1 18 is required for heterochromatin condensation in plant cells and show that H1 overexpression 19 creates heterochromatic foci in the VC progenitor cell. Taken together, our results demonstrate 20 that the natural depletion of H1 during male gametogenesis facilitates DEMETER-directed 21 DNA demethylation, heterochromatin relaxation, and TE activation.22 23 84

show abstract

Untitled

Cited by 44 publications

References 49 publications

Genome and transcriptome analysis of the grapevine (Vitis vinifera L.) WRKY gene family

Genome and transcriptome analysis of the grapevine (Vitis vinifera L.) WRKY gene family

A novel Arabidopsis marker line that strongly labels uninucleate microspores and the subsequent male gametophyte development stages

Natural depletion of H1 in sex cells causes DNA demethylation, heterochromatin decondensation and transposon activation

Contact Info

Product

Resources

About