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Kolot, Mikhail; Silberstein, Nava; Yagil, Ezra

doi:10.1023/a:1007096701720

Cited by 37 publications

(11 citation statements)

References 22 publications

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“…With regard to other recombinases, because there is no accompanying excision reaction with the C31 integrase, the integration frequency is more than two orders of magnitude higher than that of the Cre recombinase (6). Recently, integration mediated by the integrase protein of lambdoid phage HK022 has been reported in mammalian cells (17). This integrase requires host cofactors in E. coli and seems to produce a net integration frequency in mammalian cells at least an order of magnitude lower than that of the C31 integrase.…”

Section: Discussionmentioning

confidence: 99%

A phage integrase directs efficient site-specific integration in human cells

Groth

Olivares

Thyagarajan

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

459

438

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The integrase from the Streptomyces phage C31 carries out efficient recombination between the attP site in the phage genome and the attB site in the host bacterial chromosome. In this paper, we show that the enzyme also functions in human cells. A plasmid assay system was constructed that measured intramolecular integration of attP into attB. This assay was used to demonstrate that in the presence of the C31 integrase, precise unidirectional integration occurs with an efficiency of 100% in Escherichia coli and >50% in human cells. This assay system was also used to define the minimal sizes of attB and attP at 34 bp and 39 bp, respectively. Furthermore, precise and efficient intermolecular integration of an incoming plasmid bearing attP into an established Epstein-Barr virus plasmid bearing attB was documented in human cells. This work is a demonstration of efficient, site-specific, unidirectional integration in mammalian cells. These observations form the basis for site-specific integration strategies potentially useful in a broad range of genetic engineering applications.

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Section: Discussionmentioning

confidence: 99%

A phage integrase directs efficient site-specific integration in human cells

Groth

Olivares

Thyagarajan

et al. 2000

Proc. Natl. Acad. Sci. U.S.A.

459

438

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“…Motivation is one type of management activity that provides a process of encouraging oneself and other employees to pursue activities that are aimed at achieving personal or organizational goals [13,14,15].…”

mentioning

confidence: 99%

“…The motivational potential of an employee is a component of the labor potential that characterizes the employee's willingness to maximize the work return, development of competitiveness, realization of the acquired knowledge, abilities, abilities and skills in work [14]. The literature presents different theories of motivation and interpretation of types of motivation of staff.…”

mentioning

confidence: 99%

“…Based on the orientation of motivation in the subordinates in the theory of Mak-Gregor [4,13,14] ("X" and "Y"), two portraits of man, which are opposite to each other, were distinguished. Theory X forms the basis for the management and control of traditional management (through control) when the manager informs the subordinates of their obligations and applies penalties or rewards in the work process.…”

mentioning

confidence: 99%

“…Clelland [4,13,14] argued that motivation should be based on the needs of achievement, participation, power. The need to achieve is the desire to achieve the goal more effectively than in the past.…”

mentioning

confidence: 99%

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Methods of management and motivation in personnel management of the center of physical therapy and improving the quality of services for patients with orthopedic profile at the outpatient stage

Vitomskyi

Лазарєва

Федоренко

2019

HSR

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Objective: to identify the main approaches in the management and motivation of the staff of the center to improve the effectiveness of physical therapy in orthopedic disorders, to form ways to take these features into account in managing the process of physical therapy.Methods: theoretical analysis and generalization of literary sources, method of systematization of scientific information.Results. It is advisable to use a fairly wide range of management techniques in the management of staff to provide physical therapy services, since it is about the impact on a rather complex system - the team of physical therapists, which in turn have a controlling influence on the not less complex systems - patients. The use of management methods can potentially contribute to a number of positive changes in the activities of the staff of physical therapists. The additional impact of these methods will be directed at the patient, in particular, in improving the quality of services. The work of a physical therapist is rather debilitating both from the physical side and from the psychological one. Therefore, the motivation of the staff is important in the management of physical therapists, as well as other employees of the Center for the provision of physical therapy services to patients with orthopedic profile. The study of the problem of motivation has a significant theoretical and practical significance, since the most valuable resource in the current conditions of the market of physical therapy services is personnel, not equipment. Therefore, the responsibility of the leader of a successful center for the provision of physical therapy services is that the motivational goals of the employees are largely correlated with the interests of the center and led to the final result to success, which can be characterized as achieving a useful result immediately to all participants in the system " the head is a physical therapist - a patient. " In a team of physical therapy center, as in any enterprise, there are different types of workers, and the choice of one concept of motivation is mainly determined by the majority of employees of a certain type in the team. The paper outlines the features of the selection and use motivational concepts for the staff center physical therapy. The theory of motivation is considered. Economic and non-economic motivational factors are allocated within the framework of the center for providing physical therapy services, the model of motivation of the personnel is given.Conclusion. The quality and performance of physical therapists in today's market conditions depends not only on professionalism and competence, but also on management, motivation, satisfaction of their needs and loyalty.

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Site‐specific recombination in human cells catalyzed by the wild‐type integrase protein of coliphage HK022

Kolot

Meroz

Yagil

2003

Biotech & Bioengineering

Self Cite

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Abstract:The activity of the Integrase (Int) protein encoded by coliphage HK022 was tested in a human cell culture. Plasmids were constructed as substrates that carry the sites of the integration reaction (attP and attB) or the sites of excision (attL and attR). The site-specific recombination reactions were monitored in cis and in trans configurations by the expression of the green fluorescent protein (GFP) as a reporter. Cells cotransfected with the substrate plasmid(s) and with a plasmid that expresses the wild-type Int show efficient integration as well as excision in both configurations. The wild-type Int was active in the human cells without the need to supply the accessory proteins integration host factor (IHF) and excisionase (Xis) that are indispensable for the reaction in the bacterial host.

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Cited by 37 publications

References 22 publications

A phage integrase directs efficient site-specific integration in human cells

A phage integrase directs efficient site-specific integration in human cells

Methods of management and motivation in personnel management of the center of physical therapy and improving the quality of services for patients with orthopedic profile at the outpatient stage

Site‐specific recombination in human cells catalyzed by the wild‐type integrase protein of coliphage HK022

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