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Mentha piperita L. Micropropagation and the Potential Influence of Plant Growth Regulators on Volatile Organic Compound Composition

“…The properties of plant growth regulators (PGRs) on the micropropagation of several herbs, including peppermint, have been evaluated in the context of their impact on growth and development; they can be used to obtain microseedlings from protoplasts, nodal and apical segments, and leaf discs for the direct or indirect (with proliferation of callus) method of morphogenesis, and the possibility of their use to increase compounds which are attractive for the bioeconomy has also been explored [1,19,25,26]. To optimize the regeneration process from different initial peppermint explants, PGRs such as BA (6-benzyloadeninopurine), BAP(6-benzyloaminopurine), TDZ (thidiazuron), KIN (kinetin), ZEA (zeatin) and 2.4D (2,4-dichlorophenoxyacetic acid), NAA (α-naphthylacetic acid), IAA (indole-3-acetic acid), or IBA (indolyl-3-butyric acid) have mainly been used, which all have different effects on morpho-and organogenesis, playing a major role in many functions [27][28][29][30][31][32][33][34][35][36][37]. Appropriate protocols for protoplast culture were developed by Sato et al (1993) [27] and then Jullien et al (1998) [38].…”

“…Breeding protoplasts was also useful in the case of new hybrids [28,30]. Faure et al (1998) [31] described the process of the in vitro organogenesis of peppermint shoots from explants derived from leaf disks. It was observed that the regeneration occurred within 6 weeks of establishing the culture.…”