99mTechnetium labelled Escherichia coli

Evaluation of possible failure of the mononuclear phagocyte system after total splenectomy in rats

“…Several radionuclides -carbon-14, gallium-67, hydrogen-3, rhenium-188, technetium-99m, and others -have been used to label different biological structures. The physical characteristics of Tc-99m favor its preference for biological purposes since involves a simple labeling method, which, however, is a precise, effective, rapid, easily available and of low cost (Bernardo-Filho et al, 1986;Diniz et al, 1999). One of the many applications of Tc-99m is the possibility of determining the biological behavior of experimental animals in response to bacterial inoculation.…”

“…One of the many applications of Tc-99m is the possibility of determining the biological behavior of experimental animals in response to bacterial inoculation. Bacteria labeled with this technique forms a bacterium-Tc-99m complex which is stable for more than 24 hours, indicating strong binding of Tc-99m atoms with bacterial cell components and producing a rate of labeled bacteria almost always higher than 95%, favoring the reliability to the experiment (Bernardo-Filho et al, 1986;Diniz et al, 1999). The literature shows that more than 90% of the bacteria are removed from the bloodstream in the first five minutes after their inoculation (Holdsworth et al, 1989;Scher et al, 1982).…”

“…Escherichia coli (strain AB1157) was labeled with technetium-99m (Tc-99m) in the form of sodium pertechnetate (TcO 4 Na) as described previously. (Bernardo-Filho et al, 1986;Diniz et al, 1999) Briefly, a sample of E. coli culture was added to Luria and Burrows (LB) medium and incubated for 15 to 18 hours overnight at 37ºC with shaking. A 200-µl aliquot was then removed, placed in LB and incubated for an additional two hours with shaking at the same temperature (replating).…”

Influência das operações sobre o baço na distribuição da escherichia coli no sistema mononuclear fagocitário

“…Sua marcação seguiu a técnica descrita e padronizada por Diniz et al 8 A concentração bacteriana foi ajustada por meio de espectrofotômetro em porcentagem de transmitância a 580nm, que corresponde a aproximadamente 10 8 unidades formadoras de colônia por mililitro (UFC/ml).…”

Distribuição de Escherichia coli nos órgãos do sistema mononuclear fagocitário após esplenectomia total isolada ou combinada com auto-implante esplênico em rato

“…20,21 Obteve-se percentual de marcação de bactérias superior a 95% e concentração bacteriana correspondente a 10 8 unidades formadoras de colônias (UFC).…”