882 From heat maps to artificial neural networks: Multi-bioinformatics identify distinct subsets (endotypes) of psoriasis based on the metabolome of their uninvolved skin

Abstract

The exposure of hair and scalp to air pollutants has been associated with a pro-oxidant effect, increased AhR (Aryl hydrocarbon Receptor) signaling and inflammation status, reduced microvasculature in the dermal papilla, decreased melanin synthesis in the hair bulb, as well as increased sebum secretion and scalp sensitivity.First, different in vitro and ex vivo models were used to investigate the effect of pollution, including cultures of keratinocytes from scalp skin, 3D spheres of human dermal papilla cells or ex vivo scalp biopsies, that were exposed to PM2.5 or cigarette smoke, respectively. A combination of botanical extracts obtained from Pisum sativum and Salvia hispanica was evaluated for their potential effect to mitigate the negative effects of pollution exposure stress on the hair and scalp. When the extracts was applied on cells or scalp biopsies that had been exposed to these pollutants, a decrease in CYP1A1 (AhR signaling), a reduced level of IL1R1 (receptor for interleukin-1), and an increased level of VEGFA were observed.Then, a clinical study was conducted: a short-term study with 5-hour application of the combination of extracts on scalp that had been stressed by cigarette smoke exposure and a long-term study with 42 days of application of a hair serum or placebo on the scalp of 40 volunteers. The short-term study showed that oxidative damage on scalp stratum corneum was decreased. For the long-term study, evaluations were performed on scalp health and scalp soothing, and revealed that this extracts helped to rebalance the scalp's oiliness, improve the scalp's hydration and scalp barrier, and decrease scalp irritation and flaking. Taken together, the results showed a mitigating effect of a combination of botanical extracts on the negative effects of exposure to certain types of pollutants on hair and scalp. 878Keratin 17 upregulate the expression of CXCL1 in human psoriastic keratinocyte cells through going the nucleus Keratin 17(K17), as a cytoskeletal protein, is highly expressed in epithelial cells of inflammatory skin diseases and various tumor tissues. In recent years, the present of K17 in the nucleus was found in several tumors. However, such phenomenon had not been documented in inflammatory dermatoses. In our present study, K17 translocation into the nucleus of keratinocytes in psoriasis were found. Thus, greater insight into the mechanism of K17 translocation and its pathogenicity were explored. Initially, we found that K17 occurs as discrete punctate and/or in a diffuse pattern in the nucleus of keratinocytes in psoriastic lesion. Moreover, in human keratinocyte cell line HaCaT cells transfected with the fusion plasmid of wide type K17 with EGFP, K17 was aggregated as discrete punctate in the cytoplasm and then entering into the nucleus. In addition, prominent K17 retention inside the nucleus in HaCaT cells transfected with the K17 NES(nuclear export signal)(-/-) mutant plasmid were observed but K17 NLS(nuclear localization signal)(-/-) was not . Most importantly, we uncovered that CX...

Do Metabolomics and Taxonomic Barcode Markers Tell the Same Story about the Evolution of Saccharomyces sensu stricto Complex in Fermentative Environments?

“…Parallel to this evolution of the DNA-based taxonomy, the advent of proteomics and metabolomics gave new impetus to the study of the phenotype in broad sense [ 11 , 12 , 13 , 14 ]. Whereas the application of artificial intelligence [ 15 , 16 , 17 ] will surely improve the ability of metabolomics and metabolomic fingerprinting tools in the species identification, the primary role of these analytical systems is their ability to finely characterize the physiological status in various conditions, including different types of stress [ 18 ]. The ability of microbial cells to withstand stress is part of the phenotypical traits used in species description with good discriminating ability both at the specific and at the subspecific level [ 19 ].…”